##gff-version 3
##sequence-region BBa_I732913 1 1757
BBa_I732913	.	terminator	1629	1757	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I732913	.	terminator	1629	1708	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I732913	.	stem_loop	1640	1683	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732913	.	engineered_region	1629	1708	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732913	.	terminator	1717	1757	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I732913	.	polyA_site	1744	1757	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732913	.	stop_codon	1750	1750	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732913	.	engineered_region	1717	1757	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732913	.	stem_loop	1724	1743	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732913	.	engineered_region	1	888	.	+	0	ID=BBa_I732086;Name=BBa_I732086
BBa_I732913	.	promoter	835	888	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I732086
BBa_I732913	.	engineered_region	835	888	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I732913	.	non_covalent_binding_site	860	878	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I732913	.	non_covalent_binding_site	835	853	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I732913	.	promoter	877	882	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I732913	.	promoter	854	859	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I732913	.	engineered_region	1	689	.	+	0	ID=BBa_I732084;Name=BBa_I732084;Parent=BBa_I732086
BBa_I732913	.	terminator	698	777	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732086
BBa_I732913	.	stem_loop	709	752	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732913	.	engineered_region	698	777	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732913	.	terminator	786	826	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732086
BBa_I732913	.	polyA_site	813	826	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732913	.	stop_codon	819	819	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732913	.	engineered_region	786	826	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732913	.	stem_loop	793	812	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732913	.	ribosome_entry_site	897	908	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732913	.	conserved_region	901	904	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732913	.	CDS	915	1620	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_I732913	.	CDS	915	1589	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I732913	.	engineered_tag	1596	1620	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_I732913
tggtgcaaaacctttgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttca
tgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgta
cgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcag
ttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtt
tcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtga
gttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgg
gaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacg
gtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccga
catcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactcc
accggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata