LOCUS       BBa_I732918             2225 bp    DNA     linear       22-Nov-2024
DEFINITION  P_NAND2616 + lacZa/GFP with P_NOR1 + RFP
ACCESSION   BBa_I732918
VERSION     BBa_I732918.1  
FEATURES             Location/Qualifiers
     promoter        1..120
                     /label=BBa_I732301
     misc_feature    133..136
                     /label=conserved
     RBS             129..140
                     /label=BBa_B0034
     CDS             147..380
                     /label=lacZ
     misc_feature    147..149
                     /label=START
     misc_feature    375..380
                     /label=STOP
     CDS             147..380
                     /label=BBa_I732006
     misc_feature    393..396
                     /label=conserved
     RBS             389..400
                     /label=BBa_B0034
     CDS             407..1126
                     /label=GFP protein
     CDS             407..1126
                     /label=BBa_E0040
     stem_loop       1146..1189
                     /label=stem_loop
     misc_feature    1135..1214
                     /label=BBa_B0010
     terminator      1135..1214
                     /label=BBa_B0010
     stem_loop       1230..1249
                     /label=T7 TE
     misc_feature    1256..1256
                     /label=stop
     polyA_site      1250..1263
                     /label=polya
     misc_feature    1223..1263
                     /label=BBa_B0012
     terminator      1223..1263
                     /label=BBa_B0012
     misc_feature    1..1263
                     /label=BBa_I732718
     promoter        1272..1356
                     /label=BBa_I732351
     misc_feature    1369..1372
                     /label=conserved
     RBS             1365..1376
                     /label=BBa_B0034
     CDS             1383..2057
                     /label=mrfp1
     misc_feature    2064..2088
                     /label=Help:Barcodes
     CDS             1383..2088
                     /label=BBa_E1010
     stem_loop       2108..2151
                     /label=stem_loop
     misc_feature    2097..2176
                     /label=BBa_B0010
     terminator      2097..2176
                     /label=BBa_B0010
     stem_loop       2192..2211
                     /label=T7 TE
     misc_feature    2218..2218
                     /label=stop
     polyA_site      2212..2225
                     /label=polya
     misc_feature    2185..2225
                     /label=BBa_B0012
     terminator      2185..2225
                     /label=BBa_B0012
     terminator      2097..2225
                     /label=BBa_B0015
     misc_feature    1272..2225
                     /label=BBa_I732916
ORIGIN
        1 aattgtaagc gctcataatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaattgtga gcgctcataa ttggatccgg
      121 tactagagaa agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt
      181 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      241 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      301 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
      361 aaagctggct ggagtaataa tactagagaa agaggagaaa tactagatgc gtaaaggaga
      421 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      481 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      541 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      601 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      661 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      721 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      781 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      841 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      901 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      961 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
     1021 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
     1081 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
     1141 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1201 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1261 atatactaga gaattgtgag cgctcacaat ttttacactt tatgcttccg gctcgtataa
     1321 tgtgtggaaa ttgtaagcgc ttacaattgg atccggtact agagaaagag gagaaatact
     1381 agatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag
     1441 gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag
     1501 gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca
     1561 tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc
     1621 cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg
     1681 aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct
     1741 acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa
     1801 ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg
     1861 aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca
     1921 cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg
     1981 acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc
     2041 gtcactccac cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag
     2101 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2161 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2221 ttata
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