LOCUS       BBa_I732920             2430 bp    DNA     linear       07-May-2024
DEFINITION  B0015 J09855 I732835
ACCESSION   BBa_I732920
VERSION     BBa_I732920.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    138..174
                     /label=P(Lac) IQ
     promoter        140..145
                     /label=-35
     promoter        163..168
                     /label=-10
     promoter        138..174
                     /label=BBa_I14032
     misc_feature    187..190
                     /label=conserved
     RBS             183..194
                     /label=BBa_B0034
     misc_feature    201..202
                     /label=prefix
     misc_feature    374..374
                     /label=T
     misc_feature    692..692
                     /label=A
     CDS             201..950
                     /label=luxR
     misc_feature    201..956
                     /label=BBa_C0062
     misc_feature    957..981
                     /label=Help:Barcodes
     CDS             201..956
                     /label=BBa_C0062
     stem_loop       1001..1044
                     /label=stem_loop
     misc_feature    990..1069
                     /label=BBa_B0010
     terminator      990..1069
                     /label=BBa_B0010
     stem_loop       1085..1104
                     /label=T7 TE
     misc_feature    1111..1111
                     /label=stop
     polyA_site      1105..1118
                     /label=polya
     misc_feature    1078..1118
                     /label=BBa_B0012
     terminator      1078..1118
                     /label=BBa_B0012
     misc_feature    183..1118
                     /label=BBa_I0462
     misc_feature    1127..1146
                     /label=LuxR/HSL
     promoter        1146..1151
                     /label=-35
     promoter        1168..1173
                     /label=-10
     misc_feature    1179..1179
                     /label=start
     misc_feature    1127..1181
                     /label=BBa_R0062
     promoter        1127..1181
                     /label=BBa_R0062
     misc_feature    138..1181
                     /label=BBa_J09855
     misc_feature    1194..1197
                     /label=conserved
     RBS             1190..1201
                     /label=BBa_B0034
     CDS             1208..2293
                     /label=BBa_I732115
     stem_loop       2313..2356
                     /label=stem_loop
     misc_feature    2302..2381
                     /label=BBa_B0010
     terminator      2302..2381
                     /label=BBa_B0010
     stem_loop       2397..2416
                     /label=T7 TE
     misc_feature    2423..2423
                     /label=stop
     polyA_site      2417..2430
                     /label=polya
     misc_feature    2390..2430
                     /label=BBa_B0012
     terminator      2390..2430
                     /label=BBa_B0012
     misc_feature    1190..2430
                     /label=BBa_I732835
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagtgg tgcaaaacct ttcgcggtat ggcatgatag cgcctactag
      181 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      241 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      301 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      361 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      421 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      481 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      541 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      601 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      661 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      721 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      781 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      841 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      901 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      961 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1021 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1081 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1141 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1201 atactagatg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctctcgcca
     1261 aaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
     1321 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
     1381 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
     1441 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
     1501 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
     1561 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
     1621 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
     1681 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
     1741 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
     1801 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
     1861 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
     1921 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
     1981 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
     2041 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
     2101 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     2161 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     2221 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2281 cgggcagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2341 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2401 caccttcggg tgggcctttc tgcgtttata
//