##gff-version 3
##sequence-region BBa_I732920 1 2430
BBa_I732920	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I732920	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I732920	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732920	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732920	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I732920	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732920	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732920	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732920	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732920	.	engineered_region	138	1181	.	+	0	ID=BBa_J09855;Name=BBa_J09855
BBa_I732920	.	engineered_region	183	1118	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_I732920	.	CDS	201	956	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_I732920	.	engineered_region	201	956	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I732920	.	engineered_region	201	202	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I732920	.	engineered_tag	957	981	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I732920	.	point_mutation	692	692	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I732920	.	point_mutation	374	374	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I732920	.	CDS	201	950	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I732920	.	terminator	1078	1118	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_I732920	.	engineered_region	1078	1118	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732920	.	stop_codon	1111	1111	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732920	.	polyA_site	1105	1118	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732920	.	stem_loop	1085	1104	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732920	.	ribosome_entry_site	183	194	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_I732920	.	conserved_region	187	190	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732920	.	terminator	990	1069	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_I732920	.	engineered_region	990	1069	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732920	.	stem_loop	1001	1044	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732920	.	promoter	138	174	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_I732920	.	operator	138	174	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I732920	.	promoter	163	168	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I732920	.	promoter	140	145	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I732920	.	promoter	1127	1181	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_I732920	.	promoter	1146	1151	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I732920	.	promoter	1168	1173	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I732920	.	start_codon	1179	1179	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I732920	.	non_covalent_binding_site	1127	1146	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I732920	.	engineered_region	1127	1181	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I732920	.	engineered_region	1190	2430	.	+	0	ID=BBa_I732835;Name=BBa_I732835
BBa_I732920	.	terminator	2390	2430	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732835
BBa_I732920	.	engineered_region	2390	2430	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732920	.	stop_codon	2423	2423	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732920	.	polyA_site	2417	2430	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732920	.	stem_loop	2397	2416	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732920	.	ribosome_entry_site	1190	1201	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732835
BBa_I732920	.	conserved_region	1194	1197	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732920	.	terminator	2302	2381	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732835
BBa_I732920	.	engineered_region	2302	2381	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732920	.	stem_loop	2313	2356	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732920	.	CDS	1208	2293	.	+	0	ID=BBa_I732115;Name=BBa_I732115;Parent=BBa_I732835
>BBa_I732920
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggtgcaaaacctttcgcggt
atggcatgatagcgcctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataa
ttaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtga
atattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaa
tggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaa
ttaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtct
tatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagac
aactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaa
aaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaagg
aaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatg
aaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaa
attaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatact
agagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctctcgccaaacc
gtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctga
attacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggc
cctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatg
gtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcatta
actatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctc
tgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattg
ggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaat
atctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccat
gcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgcc
attaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttata
tcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctca
gggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaa
accgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaat
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata