##gff-version 3
##sequence-region BBa_I732922 1 4301
BBa_I732922	.	engineered_region	897	2137	.	+	0	ID=BBa_I732822;Name=BBa_I732822
BBa_I732922	.	CDS	915	2000	.	+	0	ID=BBa_I732102;Name=BBa_I732102;Parent=BBa_I732822
BBa_I732922	.	terminator	2009	2088	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732822
BBa_I732922	.	stem_loop	2020	2063	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732922	.	engineered_region	2009	2088	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732922	.	terminator	2097	2137	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732822
BBa_I732922	.	stop_codon	2130	2130	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732922	.	stem_loop	2104	2123	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732922	.	polyA_site	2124	2137	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732922	.	engineered_region	2097	2137	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732922	.	ribosome_entry_site	897	908	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732822
BBa_I732922	.	conserved_region	901	904	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732922	.	engineered_region	3198	4301	.	+	0	ID=BBa_I732815;Name=BBa_I732815
BBa_I732922	.	CDS	3216	4301	.	+	0	ID=BBa_I732115;Name=BBa_I732115;Parent=BBa_I732815
BBa_I732922	.	ribosome_entry_site	3198	3209	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732815
BBa_I732922	.	conserved_region	3202	3205	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732922	.	engineered_region	2146	3189	.	+	0	ID=BBa_J09855;Name=BBa_J09855
BBa_I732922	.	promoter	3135	3189	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J09855
BBa_I732922	.	non_covalent_binding_site	3135	3154	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I732922	.	promoter	3176	3181	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I732922	.	start_codon	3187	3187	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I732922	.	promoter	3154	3159	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I732922	.	engineered_region	3135	3189	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I732922	.	promoter	2146	2182	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_J09855
BBa_I732922	.	operator	2146	2182	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I732922	.	promoter	2148	2153	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I732922	.	promoter	2171	2176	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I732922	.	engineered_region	2191	3126	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_J09855
BBa_I732922	.	terminator	2998	3077	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_I732922	.	stem_loop	3009	3052	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732922	.	engineered_region	2998	3077	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732922	.	ribosome_entry_site	2191	2202	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_I732922	.	conserved_region	2195	2198	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732922	.	CDS	2209	2964	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_I732922	.	CDS	2209	2958	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I732922	.	engineered_region	2209	2964	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I732922	.	point_mutation	2382	2382	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I732922	.	engineered_region	2209	2210	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I732922	.	engineered_tag	2965	2989	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I732922	.	point_mutation	2700	2700	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I732922	.	terminator	3086	3126	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_I732922	.	stop_codon	3119	3119	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732922	.	stem_loop	3093	3112	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732922	.	polyA_site	3113	3126	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732922	.	engineered_region	3086	3126	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732922	.	engineered_region	1	888	.	+	0	ID=BBa_I732086;Name=BBa_I732086
BBa_I732922	.	promoter	835	888	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I732086
BBa_I732922	.	promoter	854	859	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I732922	.	non_covalent_binding_site	860	878	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I732922	.	promoter	877	882	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I732922	.	non_covalent_binding_site	835	853	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I732922	.	engineered_region	835	888	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I732922	.	engineered_region	1	689	.	+	0	ID=BBa_I732084;Name=BBa_I732084;Parent=BBa_I732086
BBa_I732922	.	terminator	786	826	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732086
BBa_I732922	.	stop_codon	819	819	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732922	.	stem_loop	793	812	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732922	.	polyA_site	813	826	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732922	.	engineered_region	786	826	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732922	.	terminator	698	777	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732086
BBa_I732922	.	stem_loop	709	752	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732922	.	engineered_region	698	777	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I732922
tggtgcaaaacctttgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagt
atgccggtgtctctcatcagaccgtttccaacgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagt
ggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgatt
ggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgg
gtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgca
acgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgtt
ccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgg
gcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtct
gcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgcc
atgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcaga
tggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacga
taccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtg
gaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaa
ccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttc
ccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatga
aaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatg
cttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatct
gatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatccta
tagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctcc
aaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttc
ggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccat
taattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaag
agaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgt
actgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattt
taacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgc
aagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaaaccagtaacgttatacgat
gtcgcagagtatgccggtgtctctcgccaaaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgc
gggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtc
gttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgcc
gatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatc
ttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctg
cactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggt
acgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtct
cggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcga
ctggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgcc
aacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgg
gatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggca
aaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtg
aaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcac
gacaggtttcccgactggaaagcgggcagtaataa