LOCUS       BBa_I732925             2280 bp    DNA     linear       22-Dec-2024
DEFINITION  Combined Gates (version 2)
ACCESSION   BBa_I732925
VERSION     BBa_I732925.1  
FEATURES             Location/Qualifiers
     promoter        1..178
                     /label=BBa_I732305
     misc_feature    191..194
                     /label=conserved
     RBS             187..198
                     /label=BBa_B0034
     CDS             205..438
                     /label=lacZ
     misc_feature    205..207
                     /label=START
     misc_feature    433..438
                     /label=STOP
     CDS             205..438
                     /label=BBa_I732006
     misc_feature    451..454
                     /label=conserved
     RBS             447..458
                     /label=BBa_B0034
     CDS             465..1184
                     /label=GFP protein
     CDS             465..1184
                     /label=BBa_E0040
     stem_loop       1204..1247
                     /label=stem_loop
     misc_feature    1193..1272
                     /label=BBa_B0010
     terminator      1193..1272
                     /label=BBa_B0010
     stem_loop       1288..1307
                     /label=T7 TE
     misc_feature    1314..1314
                     /label=stop
     polyA_site      1308..1321
                     /label=polya
     misc_feature    1281..1321
                     /label=BBa_B0012
     terminator      1281..1321
                     /label=BBa_B0012
     misc_feature    1..1321
                     /label=BBa_I732722
     promoter        1330..1411
                     /label=BBa_I732352
     misc_feature    1424..1427
                     /label=conserved
     RBS             1420..1431
                     /label=BBa_B0034
     CDS             1438..2112
                     /label=mrfp1
     misc_feature    2119..2143
                     /label=Help:Barcodes
     CDS             1438..2143
                     /label=BBa_E1010
     stem_loop       2163..2206
                     /label=stem_loop
     misc_feature    2152..2231
                     /label=BBa_B0010
     terminator      2152..2231
                     /label=BBa_B0010
     stem_loop       2247..2266
                     /label=T7 TE
     misc_feature    2273..2273
                     /label=stop
     polyA_site      2267..2280
                     /label=polya
     misc_feature    2240..2280
                     /label=BBa_B0012
     terminator      2240..2280
                     /label=BBa_B0012
     terminator      2152..2280
                     /label=BBa_B0015
     misc_feature    1330..2280
                     /label=BBa_I732917
ORIGIN
        1 aattgtaagc gcttacaatt gaactcgatt gtatctgtag tgctttagta gtggagttta
       61 cactttatgc ttccggctcg tataatgtgt ggaacatccc ctttatctat cgcctgtgcc
      121 cactacatca agccaaatta aacaggatta aattgtgaac gctcataatt ggatccggta
      181 ctagagaaag aggagaaata ctagatgacc atgattacgg attcactggc cgtcgtttta
      241 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      301 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      361 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
      421 agctggctgg agtaataata ctagagaaag aggagaaata ctagatgcgt aaaggagaag
      481 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      541 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      601 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      661 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      721 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      781 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      841 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      901 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      961 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     1021 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     1081 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     1141 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
     1201 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1261 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1321 atactagaga attgtgaacg ttcacaattt acactttatg cttccggctc gtataatgtg
     1381 tggaattgta agcgcttaca attggatccg gtactagaga aagaggagaa atactagatg
     1441 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
     1501 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
     1561 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
     1621 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
     1681 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
     1741 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
     1801 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
     1861 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
     1921 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
     1981 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
     2041 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
     2101 tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc
     2161 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     2221 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//