LOCUS       BBa_J29032              1086 bp    DNA     linear       29-May-2024
DEFINITION  LacI
ACCESSION   BBa_J29032
VERSION     BBa_J29032.1  
FEATURES             Location/Qualifiers
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 taataa
//
LOCUS       BBa_I732931             1305 bp    DNA     linear       29-May-2024
DEFINITION  pCR01 (R1+ R2-)
ACCESSION   BBa_I732931
VERSION     BBa_I732931.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             83..1168
                     /label=LacIq
     CDS             83..1168
                     /label=BBa_I732001
     stem_loop       1188..1231
                     /label=stem_loop
     misc_feature    1177..1256
                     /label=BBa_B0010
     terminator      1177..1256
                     /label=BBa_B0010
     stem_loop       1272..1291
                     /label=T7 TE
     misc_feature    1298..1298
                     /label=stop
     polyA_site      1292..1305
                     /label=polya
     misc_feature    1265..1305
                     /label=BBa_B0012
     terminator      1265..1305
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag aggtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtaataata ctagagccag gcatcaaata aaacgaaagg
     1201 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1261 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//