LOCUS       BBa_I732932             1302 bp    DNA     linear       10-Dec-2025
DEFINITION  pCR10 (R1- R2+)
ACCESSION   BBa_I732932
VERSION     BBa_I732932.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             83..1165
                     /label=DesR-A
     CDS             83..1165
                     /label=BBa_I732002
     stem_loop       1185..1228
                     /label=stem_loop
     misc_feature    1174..1253
                     /label=BBa_B0010
     terminator      1174..1253
                     /label=BBa_B0010
     stem_loop       1269..1288
                     /label=T7 TE
     misc_feature    1295..1295
                     /label=stop
     polyA_site      1289..1302
                     /label=polya
     misc_feature    1262..1302
                     /label=BBa_B0012
     terminator      1262..1302
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag aggtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct gtggcgaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc agtgatacta gagccaggca tcaaataaaa cgaaaggctc
     1201 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     1261 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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