LOCUS BBa_I732933 2416 bp DNA linear 05-Nov-2024 DEFINITION pCR11 (R1+ R2+) ACCESSION BBa_I732933 VERSION BBa_I732933.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 83..1168 /label=LacIq CDS 83..1168 /label=BBa_I732001 misc_feature 1181..1184 /label=conserved RBS 1177..1188 /label=BBa_B0034 CDS 1197..2279 /label=DesR-A CDS 1197..2279 /label=BBa_I732002 stem_loop 2299..2342 /label=stem_loop misc_feature 2288..2367 /label=BBa_B0010 terminator 2288..2367 /label=BBa_B0010 stem_loop 2383..2402 /label=T7 TE misc_feature 2409..2409 /label=stop polyA_site 2403..2416 /label=polya misc_feature 2376..2416 /label=BBa_B0012 terminator 2376..2416 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag aggtgaaacc agtaacgtta tacgatgtcg cagagtatgc 121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 1141 ttcccgactg gaaagcgggc agtaataata ctagagaaag aggagaaata ctagaggtga 1201 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctctgtggcg accgtttccc 1261 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 1321 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 1381 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 1441 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 1501 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 1561 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 1621 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 1681 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 1741 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 1801 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 1861 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 1921 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 1981 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 2041 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 2101 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 2161 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 2221 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgat 2281 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2341 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2401 cctttctgcg tttata // LOCUS BBa_J29032 1086 bp DNA linear 05-Nov-2024 DEFINITION LacI ACCESSION BBa_J29032 VERSION BBa_J29032.1 FEATURES Location/Qualifiers ORIGIN 1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt 61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg 121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag 181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa 301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt 361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt 481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg 661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact 721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc 781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc 901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 1081 taataa //