LOCUS BBa_I732934 4440 bp DNA linear 22-Nov-2024 DEFINITION [aTc]->R1, [AHL]->R2 ACCESSION BBa_I732934 VERSION BBa_I732934.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 CDS 64..690 /label=tetR CDS 64..690 /label=BBa_I732080 stem_loop 710..753 /label=stem_loop misc_feature 699..778 /label=BBa_B0010 terminator 699..778 /label=BBa_B0010 stem_loop 794..813 /label=T7 TE misc_feature 820..820 /label=stop polyA_site 814..827 /label=polya misc_feature 787..827 /label=BBa_B0012 terminator 787..827 /label=BBa_B0012 misc_feature 836..854 /label=TetR 1 misc_feature 836..889 /label=BBa_R0040 promoter 855..860 /label=-35 misc_feature 861..879 /label=TetR 2 promoter 878..883 /label=-10 promoter 836..889 /label=BBa_R0040 misc_feature 902..905 /label=conserved RBS 898..909 /label=BBa_B0034 CDS 918..2000 /label=DesR-A CDS 918..2000 /label=BBa_I732002 stem_loop 2020..2063 /label=stem_loop misc_feature 2009..2088 /label=BBa_B0010 terminator 2009..2088 /label=BBa_B0010 stem_loop 2104..2123 /label=T7 TE misc_feature 2130..2130 /label=stop polyA_site 2124..2137 /label=polya misc_feature 2097..2137 /label=BBa_B0012 terminator 2097..2137 /label=BBa_B0012 misc_feature 2146..2182 /label=P(Lac) IQ promoter 2148..2153 /label=-35 promoter 2171..2176 /label=-10 promoter 2146..2182 /label=BBa_I14032 misc_feature 2195..2198 /label=conserved RBS 2191..2202 /label=BBa_B0034 misc_feature 2209..2210 /label=prefix misc_feature 2382..2382 /label=T misc_feature 2700..2700 /label=A CDS 2209..2958 /label=luxR misc_feature 2209..2964 /label=BBa_C0062 misc_feature 2965..2989 /label=Help:Barcodes CDS 2209..2964 /label=BBa_C0062 stem_loop 3009..3052 /label=stem_loop misc_feature 2998..3077 /label=BBa_B0010 terminator 2998..3077 /label=BBa_B0010 stem_loop 3093..3112 /label=T7 TE misc_feature 3119..3119 /label=stop polyA_site 3113..3126 /label=polya misc_feature 3086..3126 /label=BBa_B0012 terminator 3086..3126 /label=BBa_B0012 misc_feature 3135..3154 /label=LuxR/HSL promoter 3154..3159 /label=-35 promoter 3176..3181 /label=-10 misc_feature 3187..3187 /label=start misc_feature 3135..3189 /label=BBa_R0062 promoter 3135..3189 /label=BBa_R0062 misc_feature 3202..3205 /label=conserved RBS 3198..3209 /label=BBa_B0034 CDS 3218..4303 /label=LacIq CDS 3218..4303 /label=BBa_I732001 stem_loop 4323..4366 /label=stem_loop misc_feature 4312..4391 /label=BBa_B0010 terminator 4312..4391 /label=BBa_B0010 stem_loop 4407..4426 /label=T7 TE misc_feature 4433..4433 /label=stop polyA_site 4427..4440 /label=polya misc_feature 4400..4440 /label=BBa_B0012 terminator 4400..4440 /label=BBa_B0012 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 121 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 181 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 241 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 301 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 361 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 421 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 481 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 541 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 601 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 661 caacttaaat gtgaaagtgg gtcctaataa tactagagcc aggcatcaaa taaaacgaaa 721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 781 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct 841 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagaaa 901 gaggagaaat actagaggtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 961 tctctgtggc gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 1021 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 1081 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 1141 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 1201 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 1261 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 1321 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 1381 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 1441 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 1501 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 1561 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 1621 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 1681 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 1741 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 1801 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 1861 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 1981 gactggaaag cgggcagtga tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2041 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2101 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtggtg caaaaccttt 2161 cgcggtatgg catgatagcg cctactagag aaagaggaga aatactagat gaaaaacata 2221 aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag caataatgat 2281 attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta tttactcgcg 2341 atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa ttaccctaaa 2401 aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat agtagattat 2461 tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc tgtaaataaa 2521 aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg gtttagtttc 2581 cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc agaaaaagac 2641 aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat tgttccttct 2701 ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa cgatttaacc 2761 aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg ggatatttca 2821 aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc gcaaatgaaa 2881 ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg agcaattgat 2941 tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact actagagcca 3001 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3061 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3121 tttatatact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag 3181 tcgaataaat actagagaaa gaggagaaat actagaggtg aaaccagtaa cgttatacga 3241 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag 3301 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat 3361 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac 3421 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga 3481 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa 3541 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct 3601 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct 3661 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg 3721 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc 3781 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa 3841 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca 3901 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca 3961 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat 4021 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac 4081 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc 4141 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac 4201 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 4261 gctggcacga caggtttccc gactggaaag cgggcagtaa taatactaga gccaggcatc 4321 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 4381 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata // LOCUS BBa_J29032 1086 bp DNA linear 22-Nov-2024 DEFINITION LacI ACCESSION BBa_J29032 VERSION BBa_J29032.1 FEATURES Location/Qualifiers ORIGIN 1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt 61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg 121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag 181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc 241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa 301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt 361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc 421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt 481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag 541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc 601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg 661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact 721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc 781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca 841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc 901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc 961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc 1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 1081 taataa //