##gff-version 3
##sequence-region BBa_I732934 1 4440
##sequence-region BBa_J29032 -1 -1
BBa_I732934	.	terminator	2009	2088	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732934	.	stem_loop	2020	2063	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732934	.	engineered_region	2009	2088	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732934	.	CDS	2209	2964	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I732934	.	engineered_region	2209	2210	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I732934	.	engineered_tag	2965	2989	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I732934	.	CDS	2209	2958	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I732934	.	point_mutation	2700	2700	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I732934	.	point_mutation	2382	2382	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I732934	.	engineered_region	2209	2964	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I732934	.	terminator	699	778	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732934	.	stem_loop	710	753	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732934	.	engineered_region	699	778	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732934	.	ribosome_entry_site	2191	2202	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732934	.	conserved_region	2195	2198	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732934	.	promoter	2146	2182	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_I732934	.	promoter	2148	2153	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I732934	.	promoter	2171	2176	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I732934	.	operator	2146	2182	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I732934	.	terminator	4400	4440	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732934	.	stem_loop	4407	4426	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732934	.	polyA_site	4427	4440	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732934	.	stop_codon	4433	4433	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732934	.	engineered_region	4400	4440	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732934	.	CDS	918	2000	.	+	0	ID=BBa_I732002;Name=BBa_I732002
BBa_I732934	.	CDS	918	2000	.	+	0	ID=annotation1941080;Name=DesR-A;Parent=BBa_I732002
BBa_I732934	.	ribosome_entry_site	898	909	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732934	.	conserved_region	902	905	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732934	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732934	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732934	.	promoter	3135	3189	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I732934	.	promoter	3154	3159	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I732934	.	engineered_region	3135	3189	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I732934	.	non_covalent_binding_site	3135	3154	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I732934	.	start_codon	3187	3187	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I732934	.	promoter	3176	3181	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I732934	.	terminator	4312	4391	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732934	.	stem_loop	4323	4366	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732934	.	engineered_region	4312	4391	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732934	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_I732934	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_I732934	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_I732934	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_I732934	.	ribosome_entry_site	3198	3209	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I732934	.	conserved_region	3202	3205	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I732934	.	CDS	64	690	.	+	0	ID=BBa_I732080;Name=BBa_I732080
BBa_I732934	.	CDS	64	690	.	+	0	ID=annotation1940756;Name=tetR;Parent=BBa_I732080
BBa_I732934	.	CDS	3218	4303	.	+	0	ID=BBa_I732001;Name=BBa_I732001
BBa_I732934	.	CDS	3218	4303	.	+	0	ID=annotation1940746;Name=LacIq;Parent=BBa_I732001
BBa_I732934	.	terminator	2998	3077	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I732934	.	stem_loop	3009	3052	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I732934	.	engineered_region	2998	3077	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I732934	.	terminator	3086	3126	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732934	.	stem_loop	3093	3112	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732934	.	polyA_site	3113	3126	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732934	.	stop_codon	3119	3119	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732934	.	engineered_region	3086	3126	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732934	.	terminator	787	827	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732934	.	stem_loop	794	813	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732934	.	polyA_site	814	827	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732934	.	stop_codon	820	820	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732934	.	engineered_region	787	827	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732934	.	terminator	2097	2137	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I732934	.	stem_loop	2104	2123	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I732934	.	polyA_site	2124	2137	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I732934	.	stop_codon	2130	2130	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I732934	.	engineered_region	2097	2137	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I732934	.	promoter	836	889	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I732934	.	engineered_region	836	889	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I732934	.	promoter	855	860	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I732934	.	non_covalent_binding_site	861	879	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I732934	.	promoter	878	883	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I732934	.	non_covalent_binding_site	836	854	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_I732934
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgtccagattagata
aaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgccca
gaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgag
atgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaa
gttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatga
aactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtg
gggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactg
atagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcct
tgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcctaataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatca
gtgatagagatactgagcactactagagaaagaggagaaatactagaggtgaaaccagtaacgttatacgatgtcgcag
agtatgccggtgtctctgtggcgaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaa
agtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctg
attggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaac
tgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgc
gcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaat
gttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgac
tgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcg
tctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagt
gccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatc
agatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacga
cgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagc
gtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaa
aaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcagtgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatga
aaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatg
cttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatct
gatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatccta
tagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctcc
aaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttc
ggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccat
taattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaag
agaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgt
actgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattt
taacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccagg
catcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctact
agagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgc
aagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagaggtgaaaccagtaacgttatacg
atgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaac
gcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacag
tcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcg
ccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaa
tcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcc
tgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacg
gtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgt
ctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggc
gactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttg
ccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagt
gggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctgggg
caaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactgg
tgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata
>BBa_J29032
gtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccagg
ccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggc
acaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaatt
gtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaag
cctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccagga
tgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagt
attattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgt
tagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattca
gccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatc
gttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcg
ttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaa
acaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaat
cagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttgg
ccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa