LOCUS       BBa_I732935             2140 bp    DNA     linear       06-Dec-2025
DEFINITION  [aTc] -> [R1]
ACCESSION   BBa_I732935
VERSION     BBa_I732935.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     CDS             64..690
                     /label=tetR
     CDS             64..690
                     /label=BBa_I732080
     stem_loop       710..753
                     /label=stem_loop
     misc_feature    699..778
                     /label=BBa_B0010
     terminator      699..778
                     /label=BBa_B0010
     stem_loop       794..813
                     /label=T7 TE
     misc_feature    820..820
                     /label=stop
     polyA_site      814..827
                     /label=polya
     misc_feature    787..827
                     /label=BBa_B0012
     terminator      787..827
                     /label=BBa_B0012
     misc_feature    836..854
                     /label=TetR 1
     misc_feature    836..889
                     /label=BBa_R0040
     promoter        855..860
                     /label=-35
     misc_feature    861..879
                     /label=TetR 2
     promoter        878..883
                     /label=-10
     promoter        836..889
                     /label=BBa_R0040
     misc_feature    902..905
                     /label=conserved
     RBS             898..909
                     /label=BBa_B0034
     CDS             918..2003
                     /label=LacIq
     CDS             918..2003
                     /label=BBa_I732001
     stem_loop       2023..2066
                     /label=stem_loop
     misc_feature    2012..2091
                     /label=BBa_B0010
     terminator      2012..2091
                     /label=BBa_B0010
     stem_loop       2107..2126
                     /label=T7 TE
     misc_feature    2133..2133
                     /label=stop
     polyA_site      2127..2140
                     /label=polya
     misc_feature    2100..2140
                     /label=BBa_B0012
     terminator      2100..2140
                     /label=BBa_B0012
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
      121 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct
      181 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg
      241 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
      301 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
      361 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
      421 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat
      481 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
      541 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
      601 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa
      661 caacttaaat gtgaaagtgg gtcctaataa tactagagcc aggcatcaaa taaaacgaaa
      721 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      781 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct
      841 atcagtgata gagattgaca tccctatcag tgatagagat actgagcact actagagaaa
      901 gaggagaaat actagaggtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
      961 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
     1021 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
     1081 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
     1141 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
     1201 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
     1261 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
     1321 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
     1381 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
     1441 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
     1501 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
     1561 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
     1621 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
     1681 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
     1741 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
     1801 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     1861 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
     1921 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     1981 gactggaaag cgggcagtaa taatactaga gccaggcatc aaataaaacg aaaggctcag
     2041 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2101 cacactggct caccttcggg tgggcctttc tgcgtttata
//
LOCUS       BBa_J29032              1086 bp    DNA     linear       06-Dec-2025
DEFINITION  LacI
ACCESSION   BBa_J29032
VERSION     BBa_J29032.1  
FEATURES             Location/Qualifiers
ORIGIN
        1 gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 taataa
//