##gff-version 3
##sequence-region BBa_I732950 1 3338
BBa_I732950	.	sequence_feature	3168	3173	.	+	0	ID=annotation1975775;Name=EcoRI
BBa_I732950	.	stem_loop	3139	3158	.	+	0	ID=annotation1975782;Name=Stem loop
BBa_I732950	.	sequence_alteration	34	35	.	+	0	ID=annotation1975780;Name=Deletion of AC
BBa_I732950	.	promoter	3174	3332	.	+	0	ID=annotation1975784;Name=Ptemplate1?
BBa_I732950	.	primer_binding_site	157	176	.	+	0	ID=annotation1975778;Name=Verification reverse (VR) primer binding site
BBa_I732950	.	sequence_feature	1	21	.	+	0	ID=annotation1975774;Name=BioBrick suffix
BBa_I732950	.	primer_binding_site	3050	3069	.	+	0	ID=annotation1975777;Name=Verification forward (VF2) primer binding site
BBa_I732950	.	sequence_feature	261	880	.	+	0	ID=annotation1975770;Name=pBR322 origin
BBa_I732950	.	stem_loop	3126	3129	.	+	0	ID=annotation1975783;Name=End of terminator
BBa_I732950	.	stem_loop	22	93	.	+	0	ID=annotation1975779;Name=E. coli his operon terminator
BBa_I732950	.	CDS	2067	2927	.	+	0	ID=annotation1975772;Name=beta-lactamase ampicillin resistance marker
BBa_I732950	.	sequence_feature	3333	3338	.	+	0	ID=annotation1975776;Name=XbaI
BBa_I732950	.	CDS	1097	1912	.	+	0	ID=annotation1975771;Name=kanamycin resistance marker
BBa_I732950	.	stem_loop	30	64	.	+	0	ID=annotation1975781;Name=Stem loop
BBa_I732950	.	promoter	2969	2997	.	+	0	ID=annotation1975773;Name=beta-lactamase (ampR) promoter
>BBa_I732950
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg
ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg
accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac
tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca
aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca
aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaac
tcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgta
atgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaac
atcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatcc
ggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcac
tcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaatt
acaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattct
tctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgct
tgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacc
tttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgaca
ttatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttccc
gttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatattttt
atcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaag
gatcagctcgagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcc
tgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacc
cacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt
gttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa
ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagtt
ggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttct
gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatac
gggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaag
gatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatac
tcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtat
ttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatc
atgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatg
atttctggaattcctacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttac
actttatgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaatta
aacaggattaacactctagag