##gff-version 3
##sequence-region BBa_I732952 1 2306
BBa_I732952	.	primer_binding_site	2018	2037	.	+	0	ID=annotation1975791;Name=Verification forward (VF2) primer binding site
BBa_I732952	.	sequence_feature	1	21	.	+	0	ID=annotation1975789;Name=BioBrick suffix
BBa_I732952	.	sequence_feature	2136	2141	.	+	0	ID=annotation1975788;Name=EcoRI
BBa_I732952	.	sequence_feature	2301	2306	.	+	0	ID=annotation1975790;Name=XbaI
BBa_I732952	.	stem_loop	2107	2126	.	+	0	ID=annotation1975792;Name=Stem loop
BBa_I732952	.	stem_loop	30	64	.	+	0	ID=annotation1975795;Name=Stem loop
BBa_I732952	.	sequence_feature	261	880	.	+	0	ID=annotation1975785;Name=pBR322 origin
BBa_I732952	.	stem_loop	22	93	.	+	0	ID=annotation1975794;Name=E. coli his operon terminator
BBa_I732952	.	stem_loop	2094	2097	.	+	0	ID=annotation1975793;Name=End of terminator
BBa_I732952	.	promoter	2142	2300	.	+	0	ID=annotation1975798;Name=Ptemplate1?
BBa_I732952	.	promoter	1937	1965	.	+	0	ID=annotation1975787;Name=beta-lactamase (ampR) promoter
BBa_I732952	.	primer_binding_site	157	176	.	+	0	ID=annotation1975797;Name=Verification reverse (VR) primer binding site
BBa_I732952	.	sequence_alteration	34	35	.	+	0	ID=annotation1975796;Name=Deletion of AC
BBa_I732952	.	CDS	1035	1895	.	+	0	ID=annotation1975786;Name=beta-lactamase (ampicillin resistance marker)
>BBa_I732952
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg
ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg
accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac
tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca
aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca
aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgact
ccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc
tcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg
cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgc
cattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcga
gttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccg
cagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgac
tggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat
aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct
taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgt
ttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcata
ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga
aaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgac
attaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttc
tggaattcctacccacaacccaattcgagaccggaactcgattgtatctgtagtgctttagtagtggagtttacacttt
atgcttccggctcgtataatgtgtggaacatcccctttatctatcgcctgtgcccactacatcaagccaaattaaacag
gattaacactctagag