LOCUS       BBa_I733004             1558 bp    DNA     linear       14-May-2024
DEFINITION  Produce LacZ alpha in response to AHL
ACCESSION   BBa_I733004
VERSION     BBa_I733004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     misc_feature    44..979
                     /label=BBa_I0462
     misc_feature    988..1007
                     /label=LuxR/HSL
     promoter        1007..1012
                     /label=-35
     promoter        1029..1034
                     /label=-10
     misc_feature    1040..1040
                     /label=start
     misc_feature    988..1042
                     /label=BBa_R0062
     promoter        988..1042
                     /label=BBa_R0062
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..1416
                     /label=lacZ alpha
     misc_feature    1149..1149
                     /label=T
     misc_feature    1155..1155
                     /label=A
     misc_feature    1176..1176
                     /label=C
     misc_feature    1146..1146
                     /label=C
     misc_feature    1179..1179
                     /label=G
     promoter        1094..1103
                     /label=T3 promoter
     promoter        1241..1259
                     /label=T7 promoter
     misc_feature    1267..1416
                     /label=lacZ gene fragment
     misc_feature    1069..1083
                     /label=lacZ gene fragment
     misc_feature    1069..1421
                     /label=BBa_E0033
     stem_loop       1441..1484
                     /label=stem_loop
     misc_feature    1430..1509
                     /label=BBa_B0010
     terminator      1430..1509
                     /label=BBa_B0010
     stem_loop       1525..1544
                     /label=T7 TE
     misc_feature    1551..1551
                     /label=stop
     polyA_site      1545..1558
                     /label=polya
     misc_feature    1518..1558
                     /label=BBa_B0012
     terminator      1518..1558
                     /label=BBa_B0012
     terminator      1430..1558
                     /label=BBa_B0015
     misc_feature    1051..1558
                     /label=BBa_E0433
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa
     1021 tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat gaccatgatt
     1081 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc caccgcggtg
     1141 gcggcagcac tagagctagt ggatcccccg ggctgtagaa attcgatatc aagcttatcg
     1201 ataccgtcga cctcgagggg gggcccggta cccaattcgc cctatagtga gtcgtattac
     1261 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     1321 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     1381 accgatcgcc cttcccaaca gttgcgcagc ctgaataata atactagagc caggcatcaa
     1441 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1501 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//