##gff-version 3
##sequence-region BBa_I733004 1 1558
BBa_I733004	.	promoter	988	1042	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_I733004	.	promoter	1007	1012	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I733004	.	engineered_region	988	1042	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I733004	.	non_covalent_binding_site	988	1007	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I733004	.	start_codon	1040	1040	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I733004	.	promoter	1029	1034	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I733004	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_I733004	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_I733004	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I733004	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I733004	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I733004	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I733004	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I733004	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I733004	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_I733004	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I733004	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I733004	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I733004	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I733004	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_I733004	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I733004	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_I733004	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I733004	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I733004	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
BBa_I733004	.	engineered_region	1051	1558	.	+	0	ID=BBa_E0433;Name=BBa_E0433
BBa_I733004	.	terminator	1430	1558	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0433
BBa_I733004	.	terminator	1518	1558	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I733004	.	stem_loop	1525	1544	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I733004	.	polyA_site	1545	1558	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I733004	.	stop_codon	1551	1551	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I733004	.	engineered_region	1518	1558	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I733004	.	terminator	1430	1509	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I733004	.	stem_loop	1441	1484	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I733004	.	engineered_region	1430	1509	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I733004	.	engineered_region	1069	1421	.	+	0	ID=BBa_E0033;Name=BBa_E0033;Parent=BBa_E0433
BBa_I733004	.	promoter	1241	1259	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_I733004	.	sequence_alteration	1149	1149	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_I733004	.	CDS	1069	1416	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_I733004	.	promoter	1094	1103	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_I733004	.	sequence_alteration	1155	1155	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_I733004	.	sequence_feature	1069	1083	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I733004	.	sequence_alteration	1146	1146	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_I733004	.	sequence_feature	1267	1416	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I733004	.	sequence_alteration	1179	1179	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_I733004	.	sequence_alteration	1176	1176	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_I733004	.	ribosome_entry_site	1051	1062	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0433
BBa_I733004	.	conserved_region	1055	1058	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I733004
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttg
ttatagtcgaataaatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcac
taaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcg
atatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgc
gctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatcc
ccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaataataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata