LOCUS BBa_I733006 7805 bp DNA linear 14-May-2024 DEFINITION Output Cells detect the light input and produce HSL ACCESSION BBa_I733006 VERSION BBa_I733006.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 788..812 /label=Help:Barcodes CDS 62..787 /label=BBa_I15008 misc_feature 44..812 /label=BBa_S03321 misc_feature 825..828 /label=conserved RBS 821..832 /label=BBa_B0034 CDS 839..1588 /label=PcyA misc_feature 1589..1613 /label=Help:Barcodes CDS 839..1588 /label=BBa_I15009 misc_feature 821..1613 /label=BBa_S03322 misc_feature 1626..1629 /label=conserved RBS 1622..1633 /label=BBa_B0034 CDS 1640..3877 /label=Cph8 misc_feature 3878..3902 /label=Help:Barcodes CDS 1640..3877 /label=BBa_I15010 stem_loop 3922..3965 /label=stem_loop misc_feature 3911..3990 /label=BBa_B0010 terminator 3911..3990 /label=BBa_B0010 stem_loop 4006..4025 /label=T7 TE misc_feature 4032..4032 /label=stop polyA_site 4026..4039 /label=polya misc_feature 3999..4039 /label=BBa_B0012 terminator 3999..4039 /label=BBa_B0012 misc_feature 1622..4039 /label=BBa_S03417 misc_feature 4060..4077 /label=C1 OmpR misc_feature 4081..4098 /label=C2 OmpR misc_feature 4101..4118 /label=C3 OmpR promoter 4122..4127 /label=-35 promoter 4145..4150 /label=-10 promoter 4048..4155 /label=BBa_R0082 misc_feature 4168..4171 /label=conserved RBS 4164..4175 /label=BBa_B0034 CDS 4185..4801 /label=tetR misc_feature 4802..4835 /label=SsrA misc_feature 4842..4866 /label=Help:Barcodes CDS 4182..4841 /label=BBa_C0040 stem_loop 4886..4929 /label=stem_loop misc_feature 4875..4954 /label=BBa_B0010 terminator 4875..4954 /label=BBa_B0010 stem_loop 4970..4989 /label=T7 TE misc_feature 4996..4996 /label=stop polyA_site 4990..5003 /label=polya misc_feature 4963..5003 /label=BBa_B0012 terminator 4963..5003 /label=BBa_B0012 misc_feature 5012..5030 /label=TetR 1 misc_feature 5012..5065 /label=BBa_R0040 promoter 5031..5036 /label=-35 misc_feature 5037..5055 /label=TetR 2 promoter 5054..5059 /label=-10 promoter 5012..5065 /label=BBa_R0040 misc_feature 4164..5065 /label=BBa_Q04400 misc_feature 5078..5081 /label=conserved RBS 5074..5085 /label=BBa_B0034 misc_feature 5671..5702 /label=LVA CDS 5092..5670 /label=luxI misc_feature 5092..5709 /label=BBa_C0061 misc_feature 5710..5734 /label=Help:Barcodes CDS 5092..5709 /label=BBa_C0061 stem_loop 5754..5797 /label=stem_loop misc_feature 5743..5822 /label=BBa_B0010 terminator 5743..5822 /label=BBa_B0010 stem_loop 5838..5857 /label=T7 TE misc_feature 5864..5864 /label=stop polyA_site 5858..5871 /label=polya misc_feature 5831..5871 /label=BBa_B0012 terminator 5831..5871 /label=BBa_B0012 misc_feature 5074..5871 /label=BBa_F1610 promoter 5880..5914 /label=BBa_J23100 misc_feature 5927..5930 /label=conserved RBS 5923..5934 /label=BBa_B0034 misc_feature 5941..5942 /label=prefix misc_feature 6114..6114 /label=T misc_feature 6432..6432 /label=A CDS 5941..6690 /label=luxR misc_feature 5941..6696 /label=BBa_C0062 misc_feature 6697..6721 /label=Help:Barcodes CDS 5941..6696 /label=BBa_C0062 stem_loop 6741..6784 /label=stem_loop misc_feature 6730..6809 /label=BBa_B0010 terminator 6730..6809 /label=BBa_B0010 stem_loop 6825..6844 /label=T7 TE misc_feature 6851..6851 /label=stop polyA_site 6845..6858 /label=polya misc_feature 6818..6858 /label=BBa_B0012 terminator 6818..6858 /label=BBa_B0012 misc_feature 5923..6858 /label=BBa_I0462 misc_feature 6867..6886 /label=LuxR/HSL promoter 6886..6891 /label=-35 promoter 6908..6913 /label=-10 misc_feature 6919..6919 /label=start misc_feature 6867..6921 /label=BBa_R0062 promoter 6867..6921 /label=BBa_R0062 RBS 6930..6942 /label=RBS-3\Weak RBS 6936..6939 /label=RBS misc_feature 6930..6942 /label=BBa_B0032 RBS 6930..6942 /label=BBa_B0032 CDS 6949..7668 /label=GFP protein CDS 6949..7668 /label=BBa_E0040 stem_loop 7688..7731 /label=stem_loop misc_feature 7677..7756 /label=BBa_B0010 terminator 7677..7756 /label=BBa_B0010 stem_loop 7772..7791 /label=T7 TE misc_feature 7798..7798 /label=stop polyA_site 7792..7805 /label=polya misc_feature 7765..7805 /label=BBa_B0012 terminator 7765..7805 /label=BBa_B0012 misc_feature 6930..7805 /label=BBa_E0240 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgagtgtc aacttagctt cccagttgcg ggaagggacg aaaaaatccc actccatggc 121 ggagaacgtc ggctttgtca aatgcttcct caagggcgtt gtcgagaaaa attcctaccg 181 taagctggtt ggcaatctct actttgtcta cagtgccatg gaagaggaaa tggcaaaatt 241 taaggaccat cccatcctca gccacattta cttccccgaa ctcaaccgca aacaaagcct 301 agagcaagac ctgcaattct attacggctc caactggcgg caagaagtga aaatttctgc 361 cgctggccaa gcctatgtgg accgagtccg gcaagtggcc gctacggccc ctgaattgtt 421 ggtggcccat tcctacaccc gttacctggg ggatctttcc ggcggtcaaa ttctcaagaa 481 aattgcccaa aatgccatga atctccacga tggtggcaca gctttctatg aatttgccga 541 cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa gctatgaatg atctgcccat 601 tgaccaagcc accgccgaac ggattgtgga tgaagccaat gacgcctttg ccatgaacat 661 gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg atcggcatta tggtgttcaa 721 cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt ggcctcgcca cctccgaagg 781 ctaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat 841 ggccgtcact gatttaagtt tgaccaattc ttccctgatg cctacgttga acccgatgat 901 tcaacagttg gccctggcga tcgccgctag ttggcaaagt ttacccctca agccctatca 961 attgccggag gatttgggct acgtagaagg ccgcctggaa ggggaaaagt tagtgattga 1021 aaatcggtgc taccaaacgc cccagtttcg caaaatgcat ttggagttgg ccaaggtggg 1081 caaagggttg gatattctcc actgtgtaat gtttcctgag cctttatacg gtctaccttt 1141 gtttggctgt gacattgtgg ccggccccgg tggagtaagt gcggctattg cggatctatc 1201 ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa aaatcattgg cagagctagg 1261 ccagccagaa tttgagcaac aacgggaatt gcccccctgg ggagaaatat tttctgaata 1321 ttgtttattc atccgtccca gcaatgtcac tgaagaagaa agatttgtac aaagggtagt 1381 ggactttttg caaattcatt gtcaccaatc catcgttgcc gaacccttgt ctgaagctca 1441 aactttggag caccgtcagg ggcaaattca ttactgccaa caacaacaga aaaatgataa 1501 aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg gcggaacggt atatgagcca 1561 agtcttattt gatgttatcc aataataact ctgatagtgc tagtgtagat ctctactaga 1621 gaaagaggag aaatactaga tggccaccac cgtacaactc agcgaccaat ccctccgtca 1681 gctagaaacc ctcgccatcc acaccgccca cctgattcag ccccacggtt tagtggtggt 1741 cctgcaagaa ccagacctca ccatcagcca aattagcgcc aactgcaccg gcattttagg 1801 gcgatcgcca gaggatttgt tgggcagaac cctaggggaa gtgtttgata gctttcagat 1861 tgatcccatc cagagtcgcc taacggccgg acaaatcagc agcctcaacc ccagtaaact 1921 ttgggcgcgg gtcatggggg acgactttgt catttttgac ggggtttttc atcgcaacag 1981 tgacggttta ttggtatgtg aactcgagcc agcctacact tccgataatc tgcccttcct 2041 cggtttttat cacatggcca acgctgccct gaatcggttg cgccaacaag ctaatctacg 2101 ggatttctac gatgttattg tcgaagaagt ccgccgtatg actggctttg accgggtgat 2161 gctataccgc tttgatgaaa ataaccacgg tgatgtcatt gccgaagata aacgggatga 2221 tatggaaccc tatttgggcc tgcactatcc cgaatcggat attccccaac ccgcccgtcg 2281 gctatttatc cacaacccca ttcgagtaat tcccgatgtt tatggtgtgg cggtgcccct 2341 gaccccagcg gttaacccca gcaccaaccg agcggtggat ttaacagaat ccattctgcg 2401 cagtgcgtac cattgccact tgacctatct gaaaaatatg ggggtaggag cgtctttaac 2461 catttcccta attaaggacg gccatctctg ggggctcatt gcctgccacc atcaaacccc 2521 caaagtaatt ccctttgaac tgcgtaaagc ctgcgaattt tttggtcggg tggtgtttag 2581 caacatttcc gcccaggaag atacggaaac cttcgattac cgggtgcagc tggcggagca 2641 tgaagcggtt ttattggaca aaatgaccac ggcggcggat tttgtcgaag gattaactaa 2701 tcatcccgat cgcctgttgg gattaacggg ctcccagggg gcggccattt gctttgggga 2761 aaaattgatt ttagtagggg aaaccccgga cgagaaagca gtgcaatatt tactgcaatg 2821 gttggagaat cgggaagtgc aagacgtttt cttcacctct tccctctcac aaatttatcc 2881 tgatgcagtg aattttaaat ccgtggccag tggcttattg gccattccca ttgcccgtca 2941 caactttttg ctctggtttc gccctgaagt gttgcaaacg gttaattggg gcggtgaccc 3001 aaatcatgct tacgaagcta cccaggaaga cggtaaaatc gagctccatc cccgccaatc 3061 ctttgacctc tggaaagaaa ttgtccgact ccaatctttg ccctggcaat cggtggaaat 3121 ccaaagtgcc ctggccctga aaaaggcgat cgtcaacctc attttgcgcc aggcagaaga 3181 attgcatatg gcggctggtg ttaagcaact ggcggatgac cgcacgctgc tgatggcggg 3241 ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc ctggcgactg agatgatgag 3301 cgagcaggat ggctatctgg cagaatcgat caataaagat atcgaagagt gcaacgccat 3361 cattgagcag tttatcgact acctgcgcac cgggcaggag atgccgatgg aaatggcgga 3421 tcttaatgca gtactcggtg aggtgattgc tgccgaaagt ggctatgagc gggaaattga 3481 aaccgcgctt taccccggca gcattgaagt gaaaatgcac ccgctgtcga tcaaacgcgc 3541 ggtggcgaat atggtggtca acgccgcccg ttatggcaat ggctgggtca aagtcagcag 3601 cggaacggag ccgaatcgcg cctggttcca ggtggaagat gacggtccgg gaattgcgcc 3661 ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc gacagtgcgc gcaccattag 3721 cggcacggga ttagggctgg caattgtgca gcgtatcgtg gataaccata acgggatgct 3781 ggagcttggc accagcgagc ggggcgggct ttccattcgc gcctggctgc cagtgccggt 3841 aacgcgggcg cagggcatga caaaagaagg gtaataactc tgatagtgct agtgtagatc 3901 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 3961 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4021 gggcctttct gcgtttatat actagagtcc cttgcattta cattttgaaa catctatagc 4081 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt 4141 tggggagaat ggacttacta gagaaagagg agaaatacta gatgtccaga ttagataaaa 4201 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 4261 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 4321 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 4381 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 4441 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 4501 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 4561 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 4621 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 4681 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 4741 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 4801 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 4861 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 4921 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 4981 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 5041 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatgactata 5101 atgataaaaa aatcggattt tttggcaatt ccatcggagg agtataaagg tattctaagt 5161 cttcgttatc aagtgtttaa gcaaagactt gagtgggact tagttgtaga aaataacctt 5221 gaatcagatg agtatgataa ctcaaatgca gaatatattt atgcttgtga tgatactgaa 5281 aatgtaagtg gatgctggcg tttattacct acaacaggtg attatatgct gaaaagtgtt 5341 tttcctgaat tgcttggtca acagagtgct cccaaagatc ctaatatagt cgaattaagt 5401 cgttttgctg taggtaaaaa tagctcaaag ataaataact ctgctagtga aattacaatg 5461 aaactatttg aagctatata taaacacgct gttagtcaag gtattacaga atatgtaaca 5521 gtaacatcaa cagcaataga gcgattttta aagcgtatta aagttccttg tcatcgtatt 5581 ggagacaaag aaattcatgt attaggtgat actaaatcgg ttgtattgtc tatgcctatt 5641 aatgaacagt ttaaaaaagc agtcttaaat gctgcaaacg acgaaaacta cgctttagta 5701 gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac 5761 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 5821 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt 5881 tgacggctag ctcagtccta ggtacagtgc tagctactag agaaagagga gaaatactag 5941 atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga 6001 agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat 6061 tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat 6121 aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct 6181 atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat 6241 gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact 6301 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat 6361 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta 6421 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac 6481 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct 6541 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat 6601 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca 6661 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca 6721 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 6781 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 6841 ggcctttctg cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat 6901 ggtttgttat agtcgaataa atactagagt cacacaggaa agtactagat gcgtaaagga 6961 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 7021 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 7081 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 7141 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 7201 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 7261 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 7321 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 7381 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 7441 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 7501 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 7561 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 7621 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag 7681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 7741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 7801 ttata //