LOCUS BBa_I733007 8809 bp DNA linear 12-Mar-2025 DEFINITION Weight cells either turn blue or die in response to both inputs and HSL level ACCESSION BBa_I733007 VERSION BBa_I733007.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 788..812 /label=Help:Barcodes CDS 62..787 /label=BBa_I15008 misc_feature 44..812 /label=BBa_S03321 misc_feature 825..828 /label=conserved RBS 821..832 /label=BBa_B0034 CDS 839..1588 /label=PcyA misc_feature 1589..1613 /label=Help:Barcodes CDS 839..1588 /label=BBa_I15009 misc_feature 821..1613 /label=BBa_S03322 misc_feature 1626..1629 /label=conserved RBS 1622..1633 /label=BBa_B0034 CDS 1640..3877 /label=Cph8 misc_feature 3878..3902 /label=Help:Barcodes CDS 1640..3877 /label=BBa_I15010 stem_loop 3922..3965 /label=stem_loop misc_feature 3911..3990 /label=BBa_B0010 terminator 3911..3990 /label=BBa_B0010 stem_loop 4006..4025 /label=T7 TE misc_feature 4032..4032 /label=stop polyA_site 4026..4039 /label=polya misc_feature 3999..4039 /label=BBa_B0012 terminator 3999..4039 /label=BBa_B0012 misc_feature 1622..4039 /label=BBa_S03417 misc_feature 4060..4077 /label=C1 OmpR misc_feature 4081..4098 /label=C2 OmpR misc_feature 4101..4118 /label=C3 OmpR promoter 4122..4127 /label=-35 promoter 4145..4150 /label=-10 promoter 4048..4155 /label=BBa_R0082 misc_feature 4168..4171 /label=conserved RBS 4164..4175 /label=BBa_B0034 CDS 4185..4801 /label=tetR misc_feature 4802..4835 /label=SsrA misc_feature 4842..4866 /label=Help:Barcodes CDS 4182..4841 /label=BBa_C0040 stem_loop 4886..4929 /label=stem_loop misc_feature 4875..4954 /label=BBa_B0010 terminator 4875..4954 /label=BBa_B0010 stem_loop 4970..4989 /label=T7 TE misc_feature 4996..4996 /label=stop polyA_site 4990..5003 /label=polya misc_feature 4963..5003 /label=BBa_B0012 terminator 4963..5003 /label=BBa_B0012 misc_feature 5012..5030 /label=TetR 1 misc_feature 5012..5065 /label=BBa_R0040 promoter 5031..5036 /label=-35 misc_feature 5037..5055 /label=TetR 2 promoter 5054..5059 /label=-10 promoter 5012..5065 /label=BBa_R0040 misc_feature 4164..5065 /label=BBa_Q04400 misc_feature 5078..5081 /label=conserved RBS 5074..5085 /label=BBa_B0034 misc_feature 5092..5093 /label=prefix misc_feature 5265..5265 /label=T misc_feature 5583..5583 /label=A CDS 5092..5841 /label=luxR misc_feature 5092..5847 /label=BBa_C0062 misc_feature 5848..5872 /label=Help:Barcodes CDS 5092..5847 /label=BBa_C0062 misc_feature 5074..5872 /label=BBa_S01640 misc_feature 5885..5888 /label=conserved RBS 5881..5892 /label=BBa_B0034 CDS 5899..7026 /label=LacI(ts)-LVA misc_feature 6701..6701 /label=TS mutation (G->A) misc_feature 6988..7026 /label=LVA misc_feature 7027..7051 /label=barcode from LacI (BBa_C0012) CDS 5899..7051 /label=BBa_J06501 stem_loop 7071..7114 /label=stem_loop misc_feature 7060..7139 /label=BBa_B0010 terminator 7060..7139 /label=BBa_B0010 stem_loop 7155..7174 /label=T7 TE misc_feature 7181..7181 /label=stop polyA_site 7175..7188 /label=polya misc_feature 7148..7188 /label=BBa_B0012 terminator 7148..7188 /label=BBa_B0012 misc_feature 7199..7215 /label=lac O1 promoter 7216..7221 /label=-35 misc_feature 7222..7238 /label=lac O1 promoter 7239..7244 /label=-10 misc_feature 7197..7250 /label=BBa_R0011 promoter 7197..7250 /label=BBa_R0011 misc_feature 5881..7251 /label=BBa_J06801 misc_feature 7264..7267 /label=conserved RBS 7260..7271 /label=BBa_B0034 CDS 7278..8093 /label=KanR Gene CDS 7278..8093 /label=BBa_J31003 stem_loop 8113..8156 /label=stem_loop misc_feature 8102..8181 /label=BBa_B0010 terminator 8102..8181 /label=BBa_B0010 stem_loop 8197..8216 /label=T7 TE misc_feature 8223..8223 /label=stop polyA_site 8217..8230 /label=polya misc_feature 8190..8230 /label=BBa_B0012 terminator 8190..8230 /label=BBa_B0012 terminator 8102..8230 /label=BBa_B0015 misc_feature 8239..8258 /label=LuxR/HSL promoter 8258..8263 /label=-35 promoter 8280..8285 /label=-10 misc_feature 8291..8291 /label=start misc_feature 8239..8293 /label=BBa_R0062 promoter 8239..8293 /label=BBa_R0062 misc_feature 8306..8309 /label=conserved RBS 8302..8313 /label=BBa_B0034 CDS 8320..8667 /label=lacZ alpha misc_feature 8400..8400 /label=T misc_feature 8406..8406 /label=A misc_feature 8427..8427 /label=C misc_feature 8397..8397 /label=C misc_feature 8430..8430 /label=G promoter 8345..8354 /label=T3 promoter promoter 8492..8510 /label=T7 promoter misc_feature 8518..8667 /label=lacZ gene fragment misc_feature 8320..8334 /label=lacZ gene fragment misc_feature 8320..8672 /label=BBa_E0033 stem_loop 8692..8735 /label=stem_loop misc_feature 8681..8760 /label=BBa_B0010 terminator 8681..8760 /label=BBa_B0010 stem_loop 8776..8795 /label=T7 TE misc_feature 8802..8802 /label=stop polyA_site 8796..8809 /label=polya misc_feature 8769..8809 /label=BBa_B0012 terminator 8769..8809 /label=BBa_B0012 terminator 8681..8809 /label=BBa_B0015 misc_feature 8302..8809 /label=BBa_E0433 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgagtgtc aacttagctt cccagttgcg ggaagggacg aaaaaatccc actccatggc 121 ggagaacgtc ggctttgtca aatgcttcct caagggcgtt gtcgagaaaa attcctaccg 181 taagctggtt ggcaatctct actttgtcta cagtgccatg gaagaggaaa tggcaaaatt 241 taaggaccat cccatcctca gccacattta cttccccgaa ctcaaccgca aacaaagcct 301 agagcaagac ctgcaattct attacggctc caactggcgg caagaagtga aaatttctgc 361 cgctggccaa gcctatgtgg accgagtccg gcaagtggcc gctacggccc ctgaattgtt 421 ggtggcccat tcctacaccc gttacctggg ggatctttcc ggcggtcaaa ttctcaagaa 481 aattgcccaa aatgccatga atctccacga tggtggcaca gctttctatg aatttgccga 541 cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa gctatgaatg atctgcccat 601 tgaccaagcc accgccgaac ggattgtgga tgaagccaat gacgcctttg ccatgaacat 661 gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg atcggcatta tggtgttcaa 721 cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt ggcctcgcca cctccgaagg 781 ctaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat 841 ggccgtcact gatttaagtt tgaccaattc ttccctgatg cctacgttga acccgatgat 901 tcaacagttg gccctggcga tcgccgctag ttggcaaagt ttacccctca agccctatca 961 attgccggag gatttgggct acgtagaagg ccgcctggaa ggggaaaagt tagtgattga 1021 aaatcggtgc taccaaacgc cccagtttcg caaaatgcat ttggagttgg ccaaggtggg 1081 caaagggttg gatattctcc actgtgtaat gtttcctgag cctttatacg gtctaccttt 1141 gtttggctgt gacattgtgg ccggccccgg tggagtaagt gcggctattg cggatctatc 1201 ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa aaatcattgg cagagctagg 1261 ccagccagaa tttgagcaac aacgggaatt gcccccctgg ggagaaatat tttctgaata 1321 ttgtttattc atccgtccca gcaatgtcac tgaagaagaa agatttgtac aaagggtagt 1381 ggactttttg caaattcatt gtcaccaatc catcgttgcc gaacccttgt ctgaagctca 1441 aactttggag caccgtcagg ggcaaattca ttactgccaa caacaacaga aaaatgataa 1501 aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg gcggaacggt atatgagcca 1561 agtcttattt gatgttatcc aataataact ctgatagtgc tagtgtagat ctctactaga 1621 gaaagaggag aaatactaga tggccaccac cgtacaactc agcgaccaat ccctccgtca 1681 gctagaaacc ctcgccatcc acaccgccca cctgattcag ccccacggtt tagtggtggt 1741 cctgcaagaa ccagacctca ccatcagcca aattagcgcc aactgcaccg gcattttagg 1801 gcgatcgcca gaggatttgt tgggcagaac cctaggggaa gtgtttgata gctttcagat 1861 tgatcccatc cagagtcgcc taacggccgg acaaatcagc agcctcaacc ccagtaaact 1921 ttgggcgcgg gtcatggggg acgactttgt catttttgac ggggtttttc atcgcaacag 1981 tgacggttta ttggtatgtg aactcgagcc agcctacact tccgataatc tgcccttcct 2041 cggtttttat cacatggcca acgctgccct gaatcggttg cgccaacaag ctaatctacg 2101 ggatttctac gatgttattg tcgaagaagt ccgccgtatg actggctttg accgggtgat 2161 gctataccgc tttgatgaaa ataaccacgg tgatgtcatt gccgaagata aacgggatga 2221 tatggaaccc tatttgggcc tgcactatcc cgaatcggat attccccaac ccgcccgtcg 2281 gctatttatc cacaacccca ttcgagtaat tcccgatgtt tatggtgtgg cggtgcccct 2341 gaccccagcg gttaacccca gcaccaaccg agcggtggat ttaacagaat ccattctgcg 2401 cagtgcgtac cattgccact tgacctatct gaaaaatatg ggggtaggag cgtctttaac 2461 catttcccta attaaggacg gccatctctg ggggctcatt gcctgccacc atcaaacccc 2521 caaagtaatt ccctttgaac tgcgtaaagc ctgcgaattt tttggtcggg tggtgtttag 2581 caacatttcc gcccaggaag atacggaaac cttcgattac cgggtgcagc tggcggagca 2641 tgaagcggtt ttattggaca aaatgaccac ggcggcggat tttgtcgaag gattaactaa 2701 tcatcccgat cgcctgttgg gattaacggg ctcccagggg gcggccattt gctttgggga 2761 aaaattgatt ttagtagggg aaaccccgga cgagaaagca gtgcaatatt tactgcaatg 2821 gttggagaat cgggaagtgc aagacgtttt cttcacctct tccctctcac aaatttatcc 2881 tgatgcagtg aattttaaat ccgtggccag tggcttattg gccattccca ttgcccgtca 2941 caactttttg ctctggtttc gccctgaagt gttgcaaacg gttaattggg gcggtgaccc 3001 aaatcatgct tacgaagcta cccaggaaga cggtaaaatc gagctccatc cccgccaatc 3061 ctttgacctc tggaaagaaa ttgtccgact ccaatctttg ccctggcaat cggtggaaat 3121 ccaaagtgcc ctggccctga aaaaggcgat cgtcaacctc attttgcgcc aggcagaaga 3181 attgcatatg gcggctggtg ttaagcaact ggcggatgac cgcacgctgc tgatggcggg 3241 ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc ctggcgactg agatgatgag 3301 cgagcaggat ggctatctgg cagaatcgat caataaagat atcgaagagt gcaacgccat 3361 cattgagcag tttatcgact acctgcgcac cgggcaggag atgccgatgg aaatggcgga 3421 tcttaatgca gtactcggtg aggtgattgc tgccgaaagt ggctatgagc gggaaattga 3481 aaccgcgctt taccccggca gcattgaagt gaaaatgcac ccgctgtcga tcaaacgcgc 3541 ggtggcgaat atggtggtca acgccgcccg ttatggcaat ggctgggtca aagtcagcag 3601 cggaacggag ccgaatcgcg cctggttcca ggtggaagat gacggtccgg gaattgcgcc 3661 ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc gacagtgcgc gcaccattag 3721 cggcacggga ttagggctgg caattgtgca gcgtatcgtg gataaccata acgggatgct 3781 ggagcttggc accagcgagc ggggcgggct ttccattcgc gcctggctgc cagtgccggt 3841 aacgcgggcg cagggcatga caaaagaagg gtaataactc tgatagtgct agtgtagatc 3901 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 3961 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4021 gggcctttct gcgtttatat actagagtcc cttgcattta cattttgaaa catctatagc 4081 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt 4141 tggggagaat ggacttacta gagaaagagg agaaatacta gatgtccaga ttagataaaa 4201 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 4261 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 4321 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 4381 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 4441 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 4501 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 4561 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 4621 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 4681 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 4741 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 4801 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 4861 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 4921 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 4981 gggtgggcct ttctgcgttt atatactaga gtccctatca gtgatagaga ttgacatccc 5041 tatcagtgat agagatactg agcactacta gagaaagagg agaaatacta gatgaaaaac 5101 ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat 5161 gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc 5221 gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga taattaccct 5281 aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat 5341 tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat 5401 aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt 5461 ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa 5521 gacaactata tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct 5581 tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta 5641 accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt 5701 tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg 5761 aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt 5821 gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc actactagag 5881 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag 5941 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct 6001 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc 6061 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg 6121 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt 6181 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg 6241 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag 6301 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct 6361 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg 6421 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct 6481 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag 6541 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa 6601 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg 6661 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg atgcggatat ctcggtagtg 6721 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag 6781 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 6841 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg 6901 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 6961 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct 7021 taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa 7081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 7141 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaatt 7201 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atactagaga 7261 aagaggagaa atactagatg agccatattc aacgggaaac gtcttgctcc aggccgcgat 7321 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc 7381 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga 7441 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc 7501 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat 7561 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg 7621 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc 7681 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac 7741 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg 7801 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca 7861 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta 7921 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact 7981 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata 8041 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatactaga 8101 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 8161 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 8221 tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt 8281 atagtcgaat aaatactaga gaaagaggag aaatactaga tgaccatgat tacgccaagc 8341 gcgcaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggcagca 8401 ctagagctag tggatccccc gggctgtaga aattcgatat caagcttatc gataccgtcg 8461 acctcgaggg ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca 8521 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 8581 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 8641 ccttcccaac agttgcgcag cctgaataat aatactagag ccaggcatca aataaaacga 8701 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 8761 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //