LOCUS       BBa_I735003             3172 bp    DNA     linear       25-Nov-2024
DEFINITION  Cloning CDS PHO80 in psB3C6
ACCESSION   BBa_I735003
VERSION     BBa_I735003.1  
FEATURES             Location/Qualifiers
     misc_feature    2066..2194
                     /label=BBa_B0015 terminator
     misc_feature    2203..2256
                     /label=BBa_R0040 (Ptet)
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    22..870
                     /label=p15A origin
     CDS             1232..1891
                     /label=chloramphenicol acetyl transferase (CmR resistance 
                     marker)
     stem_loop       2077..2120
                     /label=rrnB T1 terminator
     misc_feature    2044..2049
                     /label=EcoRI
     misc_feature    2050..2057
                     /label=NotI
     misc_feature    2278..2283
                     /label=XbaI
     RBS             2265..2276
                     /label=RBS
     misc_feature    2265..2276
                     /label=BBa_B0034 RBS
     promoter        2203..2256
                     /label=Ptet
     misc_feature    1..2284
                     /label=BBa_J23019
     CDS             2291..3172
                     /label=BBa_I735002
ORIGIN
        1 tactagtagc ggccgctgca gctagaaata ttttatctga ttaataagat gatcttcttg
       61 agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg
      121 cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact ggcttggagg
      181 agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg acttcaagac
      241 taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca tgtctttccg
      301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga acggggggtt
      361 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
      421 tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
      481 gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      541 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
      601 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
      661 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
      721 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
      781 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac
      841 atagtaagcc agtatacact ccgctagcgc tgatgtccgg cggtgctttt gccgttacgc
      901 accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
      961 cctcaaaaac accatcatac actaaatcag taagttggca gcatcacccg acgcactttg
     1021 cgccgaataa atacctgtga cggaagatca cttcgcagaa taaataaatc ctggtgtccc
     1081 tgttgatacc gggaagccct gggccaactt ttggcgaaaa tgagacgttg atcggcacgt
     1141 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     1201 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     1261 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     1321 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1381 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1441 tccggagttc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     1501 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     1561 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1621 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     1681 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1741 tttcacgatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1801 ggttcatcat gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1861 gtactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     1921 aacgcctggt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa
     1981 gcaaattcga cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtcaagc
     2041 ttcgaattcg cggccgcaac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     2101 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     2161 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag
     2221 attgacatcc ctatcagtga tagagatact gagcactact agagaaagag gagaaattct
     2281 agagtactag atggaaagca catcaggaga acgttccgaa aatatacatg aggatcaagg
     2341 gataccaaaa gtaattctgc ccgctgattt taataaatgc tctagaactg acctagtggt
     2401 gctcatatca cgaatgttag tatcgctgat agcaatcaat gaaaattcag caacaaagaa
     2461 atctgatgac caaattactt taacacgata ccattctaag attcctccaa acatatcaat
     2521 cttcaactat ttcatacgac tgacaaagtt ttcctcttta gaacattgtg tgcttatgac
     2581 atcactctat tatatcgatt tattgcaaac tgtgtatcct gattttacgc ttaattcgtt
     2641 gactgcccat aggtttttat taacagccac cacagtcgca acaaaaggct tatgtgattc
     2701 gttctcaaca aacgcccatt atgcaaaagt tggaggagta cgatgtcacg aattgaatat
     2761 actggagaac gattttttaa agagagtaaa ctacagaatc attccgcggg atcataacat
     2821 tacgttatgt agtatagagc aaaaacagaa aaagtttgtc atagataaaa acgcattagg
     2881 gtctctcgat ttggattctt attcttacgt taatcgtcca aaaagtggat ataatgttct
     2941 agataaatac tatcgaagaa tagttcagct ggtgggttcc tttaacgctt cacctgataa
     3001 gagtagaaag gttgattatg ttctcccgcc aaatattgat atagtgagtg aaagtggctc
     3061 ccaaactact caactaaagg ggtcgtcatc acccaattct cactcttcac aaaagcgata
     3121 ttctgaggca aaggacgcac atatctataa caagcgatca aagccagatt aa
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