BBa_I735002 1 BBa_I735002 CDS PHO80 2007-09-13T11:00:00Z 2015-08-31T04:08:00Z We overdrawed this part from the genomic sequence of S. cerevisiae. This is the coding sequence: ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATGAGGATCAAGGGATACCAAAA GTAATTCTGCCCGCTGATTTTAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCA CGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAGCAACAAAGAAATCTGATGAC CAAATTACTTTAACACGATACCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTAT TTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTGTGCTTATGACATCACTCTAT TATATCGATTTATTGCAAACTGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCAT AGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCTTATGTGATTCGTTCTCAACA AACGCCCATTATGCAAAAGTTGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAAC GATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGGATCATAACATTACGTTATGT AGTATAGAGCAAAAACAGAAAAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGAT TTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGATATAATGTTCTAGATAAATAC TATCGAAGAATAGTTCAGCTGGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAG GTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTGAAAGTGGCTCCCAAACTACT CAACTAAAGGGGTCGTCATCACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCA AAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATTAA PHO80 is a cyclin, negatively regulates phosphate metabolism; the complex Pho80p-Pho85p (cyclin-CDK complex) phosphorylates Pho4p. In our project PHO80 inhibits PHO4 activation by creating the complex Pho80p-Pho85p. false false _139_ 0 1571 9 Not in stock false No considerations! false Giovanni Russo BBa_J23019 1 BBa_J23019 EN-Ptet-rbs-X-[RFP]-SNP "ORF Expresser" 2006-08-02T11:00:00Z 2015-08-31T04:08:39Z J23018 pAC997 Variant of pBca1021 (J61003) with GFP inserted. Part J23019 is the same as J23018 but in pSB3C6. false false _52_ 0 483 95 It's complicated true N/A true John Anderson annotation1975744 1 EcoRI range1975744 1 2044 2049 annotation1975741 1 p15A origin range1975741 1 22 870 annotation1893439 1 BBa_R0040 (Ptet) range1893439 1 2203 2256 annotation1975745 1 NotI range1975745 1 2050 2057 annotation1975743 1 rrnB T1 terminator range1975743 1 2077 2120 annotation1975759 1 BBa_B0034 RBS range1975759 1 2265 2276 annotation1975742 1 chloramphenicol acetyl transferase (CmR resistance marker) range1975742 1 1232 1891 annotation1893440 1 BioBrick suffix range1893440 1 1 21 annotation1975758 1 RBS range1975758 1 2265 2276 annotation1975746 1 XbaI range1975746 1 2278 2283 annotation1893438 1 BBa_B0015 terminator range1893438 1 2066 2194 annotation1975762 1 Ptet range1975762 1 2203 2256 BBa_I735003 1 BBa_I735003 Cloning CDS PHO80 in psB3C6 2007-09-13T11:00:00Z 2015-08-31T04:08:00Z The plasmid used is BBa_J23019 and the CDS is BBa_I735002 (mutated) We cloned CDS PHO80 in plasmid psB3C6 containing a RFP. In our project this part is used as an output. false false _139_ 0 1571 9 Not in stock false We needed to mutate CDS BBa_I735002 false Giovanni Russo component2218424 1 BBa_I735002 component2218423 1 BBa_J23019 annotation2218423 1 BBa_J23019 range2218423 1 1 2284 annotation2218424 1 BBa_I735002 range2218424 1 2291 3172 BBa_I735002_sequence 1 atggaaagcacatcaggagaacgttccgaaaatatacatgaggatcaagggataccaaaagtaattctgcccgctgattttaataaatgctctagaactgacctagtggtgctcatatcacgaatgttagtatcgctgatagcaatcaatgaaaattcagcaacaaagaaatctgatgaccaaattactttaacacgataccattctaagattcctccaaacatatcaatcttcaactatttcatacgactgacaaagttttcctctttagaacattgtgtgcttatgacatcactctattatatcgatttattgcaaactgtgtatcctgattttacgcttaattcgttgactgcccataggtttttattaacagccaccacagtcgcaacaaaaggcttatgtgattcgttctcaacaaacgcccattatgcaaaagttggaggagtacgatgtcacgaattgaatatactggagaacgattttttaaagagagtaaactacagaatcattccgcgggatcataacattacgttatgtagtatagagcaaaaacagaaaaagtttgtcatagataaaaacgcattagggtctctcgatttggattcttattcttacgttaatcgtccaaaaagtggatataatgttctagataaatactatcgaagaatagttcagctggtgggttcctttaacgcttcacctgataagagtagaaaggttgattatgttctcccgccaaatattgatatagtgagtgaaagtggctcccaaactactcaactaaaggggtcgtcatcacccaattctcactcttcacaaaagcgatattctgaggcaaaggacgcacatatctataacaagcgatcaaagccagattaa BBa_J23019_sequence 1 tactagtagcggccgctgcagctagaaatattttatctgattaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgatgtccggcggtgcttttgccgttacgcaccaccccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcacccgacgcactttgcgccgaataaatacctgtgacggaagatcacttcgcagaataaataaatcctggtgtccctgttgataccgggaagccctgggccaacttttggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggagttccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttttttaaggcagttattggtgcccttaaacgcctggtgctacgcctgaataagtgataataagcggatgaatggcagaaattcgaaagcaaattcgacccggtcgtcggttcagggcagggtcgttaaatagccgcttatgtcaagcttcgaattcgcggccgcaactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattctagag BBa_I735003_sequence 1 tactagtagcggccgctgcagctagaaatattttatctgattaataagatgatcttcttgagatcgttttggtctgcgcgtaatctcttgctctgaaaacgaaaaaaccgccttgcagggcggtttttcgaaggttctctgagctaccaactctttgaaccgaggtaactggcttggaggagcgcagtcaccaaaacttgtcctttcagtttagccttaaccggcgcatgacttcaagactaactcctctaaatcaattaccagtggctgctgccagtggtgcttttgcatgtctttccgggttggactcaagacgatagttaccggataaggcgcagcggtcggactgaacggggggttcgtgcatacagtccagcttggagcgaactgcctacccggaactgagtgtcaggcgtggaatgagacaaacgcggccataacagcggaatgacaccggtaaaccgaaaggcaggaacaggagagcgcacgagggagccgccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccaccactgatttgagcgtcagatttcgtgatgcttgtcaggggggcggagcctatggaaaaacggctttgccgcggccctctcacttccctgttaagtatcttcctggcatcttccaggaaatctccgccccgttcgtaagccatttccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtgagcgaggaagcggaatatatcctgtatcacatattctgctgacgcaccggtgcagccttttttctcctgccacatgaagcacttcactgacaccctcatcagtgccaacatagtaagccagtatacactccgctagcgctgatgtccggcggtgcttttgccgttacgcaccaccccgtcagtagctgaacaggagggacagctgatagaaacagaagccactggagcacctcaaaaacaccatcatacactaaatcagtaagttggcagcatcacccgacgcactttgcgccgaataaatacctgtgacggaagatcacttcgcagaataaataaatcctggtgtccctgttgataccgggaagccctgggccaacttttggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggagttccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtctgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaatttttttaaggcagttattggtgcccttaaacgcctggtgctacgcctgaataagtgataataagcggatgaatggcagaaattcgaaagcaaattcgacccggtcgtcggttcagggcagggtcgttaaatagccgcttatgtcaagcttcgaattcgcggccgcaactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaattctagagtactagatggaaagcacatcaggagaacgttccgaaaatatacatgaggatcaagggataccaaaagtaattctgcccgctgattttaataaatgctctagaactgacctagtggtgctcatatcacgaatgttagtatcgctgatagcaatcaatgaaaattcagcaacaaagaaatctgatgaccaaattactttaacacgataccattctaagattcctccaaacatatcaatcttcaactatttcatacgactgacaaagttttcctctttagaacattgtgtgcttatgacatcactctattatatcgatttattgcaaactgtgtatcctgattttacgcttaattcgttgactgcccataggtttttattaacagccaccacagtcgcaacaaaaggcttatgtgattcgttctcaacaaacgcccattatgcaaaagttggaggagtacgatgtcacgaattgaatatactggagaacgattttttaaagagagtaaactacagaatcattccgcgggatcataacattacgttatgtagtatagagcaaaaacagaaaaagtttgtcatagataaaaacgcattagggtctctcgatttggattcttattcttacgttaatcgtccaaaaagtggatataatgttctagataaatactatcgaagaatagttcagctggtgggttcctttaacgcttcacctgataagagtagaaaggttgattatgttctcccgccaaatattgatatagtgagtgaaagtggctcccaaactactcaactaaaggggtcgtcatcacccaattctcactcttcacaaaagcgatattctgaggcaaaggacgcacatatctataacaagcgatcaaagccagattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z