LOCUS BBa_I735007 3838 bp DNA linear 22-Nov-2024 DEFINITION Cloning CDS PHO80 mutated in EX-Ptet-S-rbsRFP-P "RFP reporter" ACCESSION BBa_I735007 VERSION BBa_I735007.1 FEATURES Location/Qualifiers CDS 1..882 /label=BBa_I735004 promoter 3618..3646 /label=beta-lactamase (ampR, bla) promoter CDS 917..1597 /label=mRFP misc_feature 1631..1759 /label=BBa_B0015 misc_feature 1939..2621 /label=ColE1 origin CDS 2716..3576 /label=beta-lactamase ampicillin resistance marker stem_loop 1642..1685 /label=rrnB terminator misc_feature 1942..2561 /label=pBR322 origin misc_feature 3817..3838 /label=BioBrick prefix misc_feature 892..897 /label=SpeI misc_feature 1775..1780 /label=PstI misc_feature 1768..1775 /label=NotI RBS 899..910 /label=RBS misc_feature 899..910 /label=BBa_B0034 RBS primer_bind 1838..1857 /label=Verification reverse (VR) primer binding site primer_bind 3699..3718 /label=Verification forward (VF2) primer binding site misc_feature 3831..3831 /label=Ambiguity; should be a T misc_feature 891..3838 /label=BBa_J61002 ORIGIN 1 atggaaagca catcaggaga acgttccgaa aatatacatg aggatcaagg gataccaaaa 61 gtaattctgc ccgctgattt taataaatgc tcgagaactg acctagtggt gctcatatca 121 cgaatgttag tatcgctgat agcaatcaat gaaaattcag caacaaagaa atctgatgac 181 caaattactt taacacgata ccattctaag attcctccaa acatatcaat cttcaactat 241 ttcatacgac tgacaaagtt ttcctcttta gaacattgtg tgcttatgac atcactctat 301 tatatcgatt tattgcaaac tgtgtatcct gattttacgc ttaattcgtt gactgcccat 361 aggtttttat taacagccac cacagtcgca acaaaaggct tatgtgattc gttctcaaca 421 aacgcccatt atgcaaaagt tggaggagta cgatgtcacg aattgaatat actggagaac 481 gattttttaa agagagtaaa ctacagaatc attccgcggg atcataacat tacgttatgt 541 agtatagagc aaaaacagaa aaagtttgtc atagataaaa acgcattagg gtctctcgat 601 ttggattctt attcttacgt taatcgtcca aaaagtggat ataatgtcct agataaatac 661 tatcgaagaa tagttcagct ggtgggttcc tttaacgctt cacctgataa gagtagaaag 721 gttgattatg ttctcccgcc aaatattgat atagtgagtg aaagtggctc ccaaactact 781 caactaaagg ggtcgtcatc acccaattct cactcttcac aaaagcgata ttctgaggca 841 aaggacgcac atatctataa caagcgatca aagccagatt aatactagag tactagtgaa 901 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa 961 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg 1021 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc 1081 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca 1141 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg 1201 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga 1261 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt 1321 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg 1381 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc 1441 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac 1501 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga 1561 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc 1621 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1681 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1741 gggcctttct gcgtttatat actagaagcg gccgctgcag gcttcctcgc tcactgactc 1801 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 1861 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 1921 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 1981 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 2041 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 2101 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 2161 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 2221 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 2281 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 2341 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 2401 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 2461 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 2521 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 2581 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 2641 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 2701 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2761 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2821 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2881 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 2941 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 3001 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 3061 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 3121 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3181 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3241 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3301 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3361 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3421 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3481 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3541 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 3601 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 3661 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 3721 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata 3781 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc atctagag //