##gff-version 3
##sequence-region BBa_I735007 1 3838
BBa_I735007	.	CDS	1	882	.	+	0	ID=BBa_I735004;Name=BBa_I735004
BBa_I735007	.	plasmid_vector	891	3838	.	+	0	ID=BBa_J61002;Name=BBa_J61002
BBa_I735007	.	sequence_feature	3817	3838	.	+	0	ID=annotation1975715;Name=BioBrick prefix;Parent=BBa_J61002
BBa_I735007	.	primer_binding_site	3699	3718	.	+	0	ID=annotation1975769;Name=Verification forward (VF2) primer binding site;Parent=BBa_J61002
BBa_I735007	.	CDS	2716	3576	.	+	0	ID=annotation1892276;Name=beta-lactamase ampicillin resistance marker;Parent=BBa_J61002
BBa_I735007	.	stem_loop	1642	1685	.	+	0	ID=annotation1975713;Name=rrnB terminator;Parent=BBa_J61002
BBa_I735007	.	sequence_feature	892	897	.	+	0	ID=annotation1975716;Name=SpeI;Parent=BBa_J61002
BBa_I735007	.	sequence_feature	1942	2561	.	+	0	ID=annotation1975714;Name=pBR322 origin;Parent=BBa_J61002
BBa_I735007	.	sequence_feature	1775	1780	.	+	0	ID=annotation1975717;Name=PstI;Parent=BBa_J61002
BBa_I735007	.	engineered_region	899	910	.	+	0	ID=annotation1975767;Name=BBa_B0034 RBS;Parent=BBa_J61002
BBa_I735007	.	ribosome_entry_site	899	910	.	+	0	ID=annotation1975766;Name=RBS;Parent=BBa_J61002
BBa_I735007	.	promoter	3618	3646	.	+	0	ID=annotation1892272;Name=beta-lactamase (ampR, bla) promoter;Parent=BBa_J61002
BBa_I735007	.	engineered_region	1631	1759	.	+	0	ID=annotation1892274;Name=BBa_B0015;Parent=BBa_J61002
BBa_I735007	.	sequence_feature	1768	1775	.	+	0	ID=annotation1975718;Name=NotI;Parent=BBa_J61002
BBa_I735007	.	primer_binding_site	1838	1857	.	+	0	ID=annotation1975768;Name=Verification reverse (VR) primer binding site;Parent=BBa_J61002
BBa_I735007	.	sequence_alteration	3831	3831	.	+	0	ID=annotation1975921;Name=Ambiguity; should be a T;Parent=BBa_J61002
BBa_I735007	.	CDS	917	1597	.	+	0	ID=annotation1892273;Name=mRFP;Parent=BBa_J61002
BBa_I735007	.	sequence_feature	1939	2621	.	+	0	ID=annotation1892275;Name=ColE1 origin;Parent=BBa_J61002
>BBa_I735007
atggaaagcacatcaggagaacgttccgaaaatatacatgaggatcaagggataccaaaagtaattctgcccgctgatt
ttaataaatgctcgagaactgacctagtggtgctcatatcacgaatgttagtatcgctgatagcaatcaatgaaaattc
agcaacaaagaaatctgatgaccaaattactttaacacgataccattctaagattcctccaaacatatcaatcttcaac
tatttcatacgactgacaaagttttcctctttagaacattgtgtgcttatgacatcactctattatatcgatttattgc
aaactgtgtatcctgattttacgcttaattcgttgactgcccataggtttttattaacagccaccacagtcgcaacaaa
aggcttatgtgattcgttctcaacaaacgcccattatgcaaaagttggaggagtacgatgtcacgaattgaatatactg
gagaacgattttttaaagagagtaaactacagaatcattccgcgggatcataacattacgttatgtagtatagagcaaa
aacagaaaaagtttgtcatagataaaaacgcattagggtctctcgatttggattcttattcttacgttaatcgtccaaa
aagtggatataatgtcctagataaatactatcgaagaatagttcagctggtgggttcctttaacgcttcacctgataag
agtagaaaggttgattatgttctcccgccaaatattgatatagtgagtgaaagtggctcccaaactactcaactaaagg
ggtcgtcatcacccaattctcactcttcacaaaagcgatattctgaggcaaaggacgcacatatctataacaagcgatc
aaagccagattaatactagagtactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagtt
catgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccg
tacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgc
agttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaagg
tttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggt
gagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggtt
gggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaga
cggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaacc
gacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcact
ccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttc
ggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaa
catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccc
cctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttc
cccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggg
aagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg
cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgact
tatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtg
gtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaaga
gttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgca
gaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagg
gattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaa
agtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttc
gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgc
aatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaga
agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta
atagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctc
cggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatc
gttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccat
ccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctc
ttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcg
gggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcag
catcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgac
acggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcgga
tacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtct
aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcctt
agctttcgctaaggatgatttctggaattcgcggccgcatctagag