LOCUS       BBa_I737004             2026 bp    DNA     linear       29-Apr-2024
DEFINITION  AraC, arabinose activated, self sustaining GFP reporter.
ACCESSION   BBa_I737004
VERSION     BBa_I737004.1  
FEATURES             Location/Qualifiers
     misc_feature    4..29
                     /label=c-amp2
     misc_feature    6..44
                     /label=araO1
     misc_feature    43..72
                     /label=c-amp1
     misc_feature    73..101
                     /label=ara1 and ara2
     promoter        113..118
                     /label=-35
     promoter        136..141
                     /label=-10
     promoter        1..149
                     /label=BBa_R0080
     misc_feature    158..197
                     /label=BBa_J01010
     CDS             204..1079
                     /label=araC
     misc_feature    1080..1112
                     /label=LVA
     misc_feature    1119..1143
                     /label=Help:Barcodes
     CDS             204..1143
                     /label=BBa_C0080
     misc_feature    1156..1159
                     /label=conserved
     RBS             1152..1163
                     /label=BBa_B0034
     CDS             1170..1889
                     /label=GFP protein
     CDS             1170..1889
                     /label=BBa_E0040
     stem_loop       1909..1952
                     /label=stem_loop
     misc_feature    1898..1977
                     /label=BBa_B0010
     terminator      1898..1977
                     /label=BBa_B0010
     stem_loop       1993..2012
                     /label=T7 TE
     misc_feature    2019..2019
                     /label=stop
     polyA_site      2013..2026
                     /label=polya
     misc_feature    1986..2026
                     /label=BBa_B0012
     terminator      1986..2026
                     /label=BBa_B0012
     terminator      1898..2026
                     /label=BBa_B0015
ORIGIN
        1 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
       61 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
      121 tttatcgcaa ctctctactg tttctccatt actagagaac tagaatcacc tcttggattt
      181 gggtattaaa gaggagatac tagatggctg aagcgcaaaa tgatcccctg ctgccgggat
      241 actcgtttaa cgcccatctg gtggcgggtt taacgccgat tgaggccaat ggttatctcg
      301 atttttttat cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg
      361 gtcagggggt ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt gatattttgc
      421 tgttcccgcc aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc
      481 accagtgggt ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa
      541 tatttgccaa tacgggtttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc
      601 tgtttgggca aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga
      661 taaatctgct tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc
      721 caccgatgga taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca
      781 gcaattttga tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg cgtctgtcac
      841 atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgcatca
      901 gccaggcgaa gctgcttttg agcactaccc ggatgcctat cgccaccgtc ggtcgcaatg
      961 ttggttttga cgatcaactc tatttctcgc gagtatttaa aaaatgcacc ggggccagcc
     1021 cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcag
     1081 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat
     1141 cactactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
     1201 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     1261 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     1321 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
     1381 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
     1441 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
     1501 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
     1561 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
     1621 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
     1681 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     1741 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
     1801 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
     1861 atggcatgga tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag
     1921 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1981 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//