LOCUS BBa_I737007 6518 bp DNA linear 04-May-2024 DEFINITION Constitutive expression of all the accessory parts needed for our plasmid ACCESSION BBa_I737007 VERSION BBa_I737007.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 misc_feature 874..877 /label=conserved RBS 870..881 /label=BBa_B0034 CDS 888..2015 /label=LacI(ts)-LVA misc_feature 1690..1690 /label=TS mutation (G->A) misc_feature 1977..2015 /label=LVA misc_feature 2016..2040 /label=barcode from LacI (BBa_C0012) CDS 888..2040 /label=BBa_J06501 stem_loop 2060..2103 /label=stem_loop misc_feature 2049..2128 /label=BBa_B0010 terminator 2049..2128 /label=BBa_B0010 stem_loop 2144..2163 /label=T7 TE misc_feature 2170..2170 /label=stop polyA_site 2164..2177 /label=polya misc_feature 2137..2177 /label=BBa_B0012 terminator 2137..2177 /label=BBa_B0012 terminator 2049..2177 /label=BBa_B0015 promoter 2186..2315 /label=BBa_I13453 misc_feature 2328..2331 /label=conserved RBS 2324..2335 /label=BBa_B0034 misc_feature 3068..3092 /label=Help:Barcodes CDS 2342..3067 /label=BBa_I15008 misc_feature 3105..3108 /label=conserved RBS 3101..3112 /label=BBa_B0034 CDS 3119..3868 /label=PcyA misc_feature 3869..3893 /label=Help:Barcodes CDS 3119..3868 /label=BBa_I15009 stem_loop 3913..3956 /label=stem_loop misc_feature 3902..3981 /label=BBa_B0010 terminator 3902..3981 /label=BBa_B0010 stem_loop 3997..4016 /label=T7 TE misc_feature 4023..4023 /label=stop polyA_site 4017..4030 /label=polya misc_feature 3990..4030 /label=BBa_B0012 terminator 3990..4030 /label=BBa_B0012 misc_feature 4039..4057 /label=TetR 1 misc_feature 4039..4092 /label=BBa_R0040 promoter 4058..4063 /label=-35 misc_feature 4064..4082 /label=TetR 2 promoter 4081..4086 /label=-10 promoter 4039..4092 /label=BBa_R0040 misc_feature 4105..4108 /label=conserved RBS 4101..4112 /label=BBa_B0034 CDS 4119..6356 /label=Cph8 misc_feature 6357..6381 /label=Help:Barcodes CDS 4119..6356 /label=BBa_I15010 stem_loop 6401..6444 /label=stem_loop misc_feature 6390..6469 /label=BBa_B0010 terminator 6390..6469 /label=BBa_B0010 stem_loop 6485..6504 /label=T7 TE misc_feature 6511..6511 /label=stop polyA_site 6505..6518 /label=polya misc_feature 6478..6518 /label=BBa_B0012 terminator 6478..6518 /label=BBa_B0012 misc_feature 2186..6518 /label=BBa_M30109 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gtgaatgtga 901 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 961 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 1021 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 1081 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 1141 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 1201 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 1261 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 1321 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 1381 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 1441 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 1501 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 1561 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 1621 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 1681 tgcgcgttga tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 1741 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 1801 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 1861 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 1921 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg 1981 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc 2041 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 2101 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2161 gcctttctgc gtttatatac tagagacatt gattatttgc acggcgtcac actttgctat 2221 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc 2281 tctactgttt ctccataccg tttttttggg ctagctacta gagaaagagg agaaatacta 2341 gatgagtgtc aacttagctt cccagttgcg ggaagggacg aaaaaatccc actccatggc 2401 ggagaacgtc ggctttgtca aatgcttcct caagggcgtt gtcgagaaaa attcctaccg 2461 taagctggtt ggcaatctct actttgtcta cagtgccatg gaagaggaaa tggcaaaatt 2521 taaggaccat cccatcctca gccacattta cttccccgaa ctcaaccgca aacaaagcct 2581 agagcaagac ctgcaattct attacggctc caactggcgg caagaagtga aaatttctgc 2641 cgctggccaa gcctatgtgg accgagtccg gcaagtggcc gctacggccc ctgaattgtt 2701 ggtggcccat tcctacaccc gttacctggg ggatctttcc ggcggtcaaa ttctcaagaa 2761 aattgcccaa aatgccatga atctccacga tggtggcaca gctttctatg aatttgccga 2821 cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa gctatgaatg atctgcccat 2881 tgaccaagcc accgccgaac ggattgtgga tgaagccaat gacgcctttg ccatgaacat 2941 gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg atcggcatta tggtgttcaa 3001 cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt ggcctcgcca cctccgaagg 3061 ctaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat 3121 ggccgtcact gatttaagtt tgaccaattc ttccctgatg cctacgttga acccgatgat 3181 tcaacagttg gccctggcga tcgccgctag ttggcaaagt ttacccctca agccctatca 3241 attgccggag gatttgggct acgtagaagg ccgcctggaa ggggaaaagt tagtgattga 3301 aaatcggtgc taccaaacgc cccagtttcg caaaatgcat ttggagttgg ccaaggtggg 3361 caaagggttg gatattctcc actgtgtaat gtttcctgag cctttatacg gtctaccttt 3421 gtttggctgt gacattgtgg ccggccccgg tggagtaagt gcggctattg cggatctatc 3481 ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa aaatcattgg cagagctagg 3541 ccagccagaa tttgagcaac aacgggaatt gcccccctgg ggagaaatat tttctgaata 3601 ttgtttattc atccgtccca gcaatgtcac tgaagaagaa agatttgtac aaagggtagt 3661 ggactttttg caaattcatt gtcaccaatc catcgttgcc gaacccttgt ctgaagctca 3721 aactttggag caccgtcagg ggcaaattca ttactgccaa caacaacaga aaaatgataa 3781 aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg gcggaacggt atatgagcca 3841 agtcttattt gatgttatcc aataataact ctgatagtgc tagtgtagat ctctactaga 3901 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 3961 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 4021 tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat cagtgataga 4081 gatactgagc actactagag aaagaggaga aatactagat ggccaccacc gtacaactca 4141 gcgaccaatc cctccgtcag ctagaaaccc tcgccatcca caccgcccac ctgattcagc 4201 cccacggttt agtggtggtc ctgcaagaac cagacctcac catcagccaa attagcgcca 4261 actgcaccgg cattttaggg cgatcgccag aggatttgtt gggcagaacc ctaggggaag 4321 tgtttgatag ctttcagatt gatcccatcc agagtcgcct aacggccgga caaatcagca 4381 gcctcaaccc cagtaaactt tgggcgcggg tcatggggga cgactttgtc atttttgacg 4441 gggtttttca tcgcaacagt gacggtttat tggtatgtga actcgagcca gcctacactt 4501 ccgataatct gcccttcctc ggtttttatc acatggccaa cgctgccctg aatcggttgc 4561 gccaacaagc taatctacgg gatttctacg atgttattgt cgaagaagtc cgccgtatga 4621 ctggctttga ccgggtgatg ctataccgct ttgatgaaaa taaccacggt gatgtcattg 4681 ccgaagataa acgggatgat atggaaccct atttgggcct gcactatccc gaatcggata 4741 ttccccaacc cgcccgtcgg ctatttatcc acaaccccat tcgagtaatt cccgatgttt 4801 atggtgtggc ggtgcccctg accccagcgg ttaaccccag caccaaccga gcggtggatt 4861 taacagaatc cattctgcgc agtgcgtacc attgccactt gacctatctg aaaaatatgg 4921 gggtaggagc gtctttaacc atttccctaa ttaaggacgg ccatctctgg gggctcattg 4981 cctgccacca tcaaaccccc aaagtaattc cctttgaact gcgtaaagcc tgcgaatttt 5041 ttggtcgggt ggtgtttagc aacatttccg cccaggaaga tacggaaacc ttcgattacc 5101 gggtgcagct ggcggagcat gaagcggttt tattggacaa aatgaccacg gcggcggatt 5161 ttgtcgaagg attaactaat catcccgatc gcctgttggg attaacgggc tcccaggggg 5221 cggccatttg ctttggggaa aaattgattt tagtagggga aaccccggac gagaaagcag 5281 tgcaatattt actgcaatgg ttggagaatc gggaagtgca agacgttttc ttcacctctt 5341 ccctctcaca aatttatcct gatgcagtga attttaaatc cgtggccagt ggcttattgg 5401 ccattcccat tgcccgtcac aactttttgc tctggtttcg ccctgaagtg ttgcaaacgg 5461 ttaattgggg cggtgaccca aatcatgctt acgaagctac ccaggaagac ggtaaaatcg 5521 agctccatcc ccgccaatcc tttgacctct ggaaagaaat tgtccgactc caatctttgc 5581 cctggcaatc ggtggaaatc caaagtgccc tggccctgaa aaaggcgatc gtcaacctca 5641 ttttgcgcca ggcagaagaa ttgcatatgg cggctggtgt taagcaactg gcggatgacc 5701 gcacgctgct gatggcgggg gtaagtcacg acttgcgcac gccgctgacg cgtattcgcc 5761 tggcgactga gatgatgagc gagcaggatg gctatctggc agaatcgatc aataaagata 5821 tcgaagagtg caacgccatc attgagcagt ttatcgacta cctgcgcacc gggcaggaga 5881 tgccgatgga aatggcggat cttaatgcag tactcggtga ggtgattgct gccgaaagtg 5941 gctatgagcg ggaaattgaa accgcgcttt accccggcag cattgaagtg aaaatgcacc 6001 cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa cgccgcccgt tatggcaatg 6061 gctgggtcaa agtcagcagc ggaacggagc cgaatcgcgc ctggttccag gtggaagatg 6121 acggtccggg aattgcgccg gaacaacgta agcacctgtt ccagccgttt gtccgcggcg 6181 acagtgcgcg caccattagc ggcacgggat tagggctggc aattgtgcag cgtatcgtgg 6241 ataaccataa cgggatgctg gagcttggca ccagcgagcg gggcgggctt tccattcgcg 6301 cctggctgcc agtgccggta acgcgggcgc agggcatgac aaaagaaggg taataactct 6361 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 6421 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 6481 cactggctca ccttcgggtg ggcctttctg cgtttata //