##gff-version 3
##sequence-region BBa_I737007 1 6518
BBa_I737007	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I737007	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737007	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I737007	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I737007	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I737007	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I737007	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I737007	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I737007	.	terminator	2049	2177	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_I737007	.	terminator	2049	2128	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I737007	.	stem_loop	2060	2103	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737007	.	engineered_region	2049	2128	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737007	.	terminator	2137	2177	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I737007	.	polyA_site	2164	2177	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737007	.	stem_loop	2144	2163	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737007	.	engineered_region	2137	2177	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737007	.	stop_codon	2170	2170	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737007	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I737007	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I737007	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I737007	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I737007	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I737007	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I737007	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I737007	.	engineered_region	2186	6518	.	+	0	ID=BBa_M30109;Name=BBa_M30109
BBa_I737007	.	terminator	6390	6469	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_I737007	.	stem_loop	6401	6444	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737007	.	engineered_region	6390	6469	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737007	.	CDS	3119	3868	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_M30109
BBa_I737007	.	engineered_tag	3869	3893	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_I737007	.	CDS	3119	3868	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_I737007	.	ribosome_entry_site	3101	3112	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_I737007	.	conserved_region	3105	3108	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737007	.	terminator	3990	4030	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_I737007	.	polyA_site	4017	4030	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737007	.	stem_loop	3997	4016	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737007	.	engineered_region	3990	4030	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737007	.	stop_codon	4023	4023	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737007	.	terminator	6478	6518	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_M30109
BBa_I737007	.	polyA_site	6505	6518	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737007	.	stem_loop	6485	6504	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737007	.	engineered_region	6478	6518	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737007	.	stop_codon	6511	6511	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737007	.	CDS	4119	6356	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_M30109
BBa_I737007	.	CDS	4119	6356	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_I737007	.	engineered_tag	6357	6381	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_I737007	.	promoter	2186	2315	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_M30109
BBa_I737007	.	ribosome_entry_site	4101	4112	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_I737007	.	conserved_region	4105	4108	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737007	.	CDS	2342	3067	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_M30109
BBa_I737007	.	engineered_tag	3068	3092	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_I737007	.	promoter	4039	4092	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_M30109
BBa_I737007	.	promoter	4058	4063	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I737007	.	promoter	4081	4086	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I737007	.	non_covalent_binding_site	4039	4057	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I737007	.	engineered_region	4039	4092	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I737007	.	non_covalent_binding_site	4064	4082	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I737007	.	terminator	3902	3981	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_M30109
BBa_I737007	.	stem_loop	3913	3956	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737007	.	engineered_region	3902	3981	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737007	.	ribosome_entry_site	2324	2335	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_M30109
BBa_I737007	.	conserved_region	2328	2331	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737007	.	CDS	888	2040	.	+	0	ID=BBa_J06501;Name=BBa_J06501
BBa_I737007	.	sequence_alteration	1690	1690	.	+	0	ID=annotation1530900;Name=TS mutation (G->A);Parent=BBa_J06501
BBa_I737007	.	engineered_tag	2016	2040	.	+	0	ID=annotation1530914;Name=barcode from LacI (BBa_C0012);Parent=BBa_J06501
BBa_I737007	.	CDS	888	2015	.	+	0	ID=annotation1530899;Name=LacI(ts)-LVA;Parent=BBa_J06501
BBa_I737007	.	tag	1977	2015	.	+	0	ID=annotation1530907;Name=LVA;Parent=BBa_J06501
BBa_I737007	.	ribosome_entry_site	870	881	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I737007	.	conserved_region	874	877	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I737007
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatc
agaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcgga
gctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagt
ctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgt
cgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgat
cattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgat
gtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcg
cattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggca
taaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaa
accatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgc
gcgccattaccgagtccgggctgcgcgttgatgcggatatctcggtagtgggatacgacgataccgaagacagctcatg
ttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactc
tctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaata
cgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca
ggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcg
ggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaa
aattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggacc
atcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacgg
ctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacg
gcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattg
cccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaa
aaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgac
gcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaaca
gcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgcta
gtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatg
cctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatc
aattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaac
gccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcct
gagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatc
tatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttga
gcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaa
gaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctg
aagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggt
actggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactc
tgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagagga
gaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcc
cacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgca
ccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcc
catccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgac
tttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccg
ataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacggga
tttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaat
aaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggata
ttccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccct
gaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccac
ttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctca
ttgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtt
tagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattg
gacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctccc
agggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttact
gcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaat
tttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgt
tgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatcc
ccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgcc
ctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaac
tggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgac
tgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgag
cagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtga
ttgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgct
gtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcgga
acggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttcc
agccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtgga
taaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggta
acgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata