LOCUS BBa_I737009 5332 bp DNA linear 26-Nov-2024 DEFINITION Light -> GFP ACCESSION BBa_I737009 VERSION BBa_I737009.1 FEATURES Location/Qualifiers misc_feature 11..28 /label=F1 OmpR misc_feature 31..48 /label=F2 OmpR misc_feature 51..68 /label=F3 OmpR promoter 73..78 /label=-35 promoter 88..93 /label=-10 promoter 1..108 /label=BBa_R0084 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 135..854 /label=GFP protein CDS 135..854 /label=BBa_E0040 stem_loop 874..917 /label=stem_loop misc_feature 863..942 /label=BBa_B0010 terminator 863..942 /label=BBa_B0010 stem_loop 958..977 /label=T7 TE misc_feature 984..984 /label=stop polyA_site 978..991 /label=polya misc_feature 951..991 /label=BBa_B0012 terminator 951..991 /label=BBa_B0012 misc_feature 1..991 /label=BBa_I737008 promoter 1000..1129 /label=BBa_I13453 misc_feature 1142..1145 /label=conserved RBS 1138..1149 /label=BBa_B0034 misc_feature 1882..1906 /label=Help:Barcodes CDS 1156..1881 /label=BBa_I15008 misc_feature 1919..1922 /label=conserved RBS 1915..1926 /label=BBa_B0034 CDS 1933..2682 /label=PcyA misc_feature 2683..2707 /label=Help:Barcodes CDS 1933..2682 /label=BBa_I15009 stem_loop 2727..2770 /label=stem_loop misc_feature 2716..2795 /label=BBa_B0010 terminator 2716..2795 /label=BBa_B0010 stem_loop 2811..2830 /label=T7 TE misc_feature 2837..2837 /label=stop polyA_site 2831..2844 /label=polya misc_feature 2804..2844 /label=BBa_B0012 terminator 2804..2844 /label=BBa_B0012 misc_feature 2853..2871 /label=TetR 1 misc_feature 2853..2906 /label=BBa_R0040 promoter 2872..2877 /label=-35 misc_feature 2878..2896 /label=TetR 2 promoter 2895..2900 /label=-10 promoter 2853..2906 /label=BBa_R0040 misc_feature 2919..2922 /label=conserved RBS 2915..2926 /label=BBa_B0034 CDS 2933..5170 /label=Cph8 misc_feature 5171..5195 /label=Help:Barcodes CDS 2933..5170 /label=BBa_I15010 stem_loop 5215..5258 /label=stem_loop misc_feature 5204..5283 /label=BBa_B0010 terminator 5204..5283 /label=BBa_B0010 stem_loop 5299..5318 /label=T7 TE misc_feature 5325..5325 /label=stop polyA_site 5319..5332 /label=polya misc_feature 5292..5332 /label=BBa_B0012 terminator 5292..5332 /label=BBa_B0012 misc_feature 1000..5332 /label=BBa_M30109 ORIGIN 1 cttaaatttt acttttggtt acatattttt tctttttgaa accaaatctt tatctttgta 61 gcactttcac ggtagcgaaa cgttagtttg aatggaaaga tgcctgcata ctagagaaag 121 aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg 181 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 241 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc 301 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag 361 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa 421 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag 481 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca 541 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca 601 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg 661 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac 721 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag 781 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac 841 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 901 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 961 tcaccttcgg gtgggccttt ctgcgtttat atactagaga cattgattat ttgcacggcg 1021 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct 1081 ttttatcgca actctctact gtttctccat accgtttttt tgggctagct actagagaaa 1141 gaggagaaat actagatgag tgtcaactta gcttcccagt tgcgggaagg gacgaaaaaa 1201 tcccactcca tggcggagaa cgtcggcttt gtcaaatgct tcctcaaggg cgttgtcgag 1261 aaaaattcct accgtaagct ggttggcaat ctctactttg tctacagtgc catggaagag 1321 gaaatggcaa aatttaagga ccatcccatc ctcagccaca tttacttccc cgaactcaac 1381 cgcaaacaaa gcctagagca agacctgcaa ttctattacg gctccaactg gcggcaagaa 1441 gtgaaaattt ctgccgctgg ccaagcctat gtggaccgag tccggcaagt ggccgctacg 1501 gcccctgaat tgttggtggc ccattcctac acccgttacc tgggggatct ttccggcggt 1561 caaattctca agaaaattgc ccaaaatgcc atgaatctcc acgatggtgg cacagctttc 1621 tatgaatttg ccgacattga tgacgaaaag gcttttaaaa atacctaccg tcaagctatg 1681 aatgatctgc ccattgacca agccaccgcc gaacggattg tggatgaagc caatgacgcc 1741 tttgccatga acatgaaaat gttcaacgaa cttgaaggca acctgatcaa ggcgatcggc 1801 attatggtgt tcaacagcct cacccgtcgc cgcagtcaag gcagcaccga agttggcctc 1861 gccacctccg aaggctaata acgctgatag tgctagtgta gatcgctact agagaaagag 1921 gagaaatact agatggccgt cactgattta agtttgacca attcttccct gatgcctacg 1981 ttgaacccga tgattcaaca gttggccctg gcgatcgccg ctagttggca aagtttaccc 2041 ctcaagccct atcaattgcc ggaggatttg ggctacgtag aaggccgcct ggaaggggaa 2101 aagttagtga ttgaaaatcg gtgctaccaa acgccccagt ttcgcaaaat gcatttggag 2161 ttggccaagg tgggcaaagg gttggatatt ctccactgtg taatgtttcc tgagccttta 2221 tacggtctac ctttgtttgg ctgtgacatt gtggccggcc ccggtggagt aagtgcggct 2281 attgcggatc tatcccccac ccaaagcgat cgccaattgc ccgcagcgta ccaaaaatca 2341 ttggcagagc taggccagcc agaatttgag caacaacggg aattgccccc ctggggagaa 2401 atattttctg aatattgttt attcatccgt cccagcaatg tcactgaaga agaaagattt 2461 gtacaaaggg tagtggactt tttgcaaatt cattgtcacc aatccatcgt tgccgaaccc 2521 ttgtctgaag ctcaaacttt ggagcaccgt caggggcaaa ttcattactg ccaacaacaa 2581 cagaaaaatg ataaaacccg tcgggtactg gaaaaagctt ttggggaagc ttgggcggaa 2641 cggtatatga gccaagtctt atttgatgtt atccaataat aactctgata gtgctagtgt 2701 agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2761 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2821 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc 2881 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatggccac 2941 caccgtacaa ctcagcgacc aatccctccg tcagctagaa accctcgcca tccacaccgc 3001 ccacctgatt cagccccacg gtttagtggt ggtcctgcaa gaaccagacc tcaccatcag 3061 ccaaattagc gccaactgca ccggcatttt agggcgatcg ccagaggatt tgttgggcag 3121 aaccctaggg gaagtgtttg atagctttca gattgatccc atccagagtc gcctaacggc 3181 cggacaaatc agcagcctca accccagtaa actttgggcg cgggtcatgg gggacgactt 3241 tgtcattttt gacggggttt ttcatcgcaa cagtgacggt ttattggtat gtgaactcga 3301 gccagcctac acttccgata atctgccctt cctcggtttt tatcacatgg ccaacgctgc 3361 cctgaatcgg ttgcgccaac aagctaatct acgggatttc tacgatgtta ttgtcgaaga 3421 agtccgccgt atgactggct ttgaccgggt gatgctatac cgctttgatg aaaataacca 3481 cggtgatgtc attgccgaag ataaacggga tgatatggaa ccctatttgg gcctgcacta 3541 tcccgaatcg gatattcccc aacccgcccg tcggctattt atccacaacc ccattcgagt 3601 aattcccgat gtttatggtg tggcggtgcc cctgacccca gcggttaacc ccagcaccaa 3661 ccgagcggtg gatttaacag aatccattct gcgcagtgcg taccattgcc acttgaccta 3721 tctgaaaaat atgggggtag gagcgtcttt aaccatttcc ctaattaagg acggccatct 3781 ctgggggctc attgcctgcc accatcaaac ccccaaagta attccctttg aactgcgtaa 3841 agcctgcgaa ttttttggtc gggtggtgtt tagcaacatt tccgcccagg aagatacgga 3901 aaccttcgat taccgggtgc agctggcgga gcatgaagcg gttttattgg acaaaatgac 3961 cacggcggcg gattttgtcg aaggattaac taatcatccc gatcgcctgt tgggattaac 4021 gggctcccag ggggcggcca tttgctttgg ggaaaaattg attttagtag gggaaacccc 4081 ggacgagaaa gcagtgcaat atttactgca atggttggag aatcgggaag tgcaagacgt 4141 tttcttcacc tcttccctct cacaaattta tcctgatgca gtgaatttta aatccgtggc 4201 cagtggctta ttggccattc ccattgcccg tcacaacttt ttgctctggt ttcgccctga 4261 agtgttgcaa acggttaatt ggggcggtga cccaaatcat gcttacgaag ctacccagga 4321 agacggtaaa atcgagctcc atccccgcca atcctttgac ctctggaaag aaattgtccg 4381 actccaatct ttgccctggc aatcggtgga aatccaaagt gccctggccc tgaaaaaggc 4441 gatcgtcaac ctcattttgc gccaggcaga agaattgcat atggcggctg gtgttaagca 4501 actggcggat gaccgcacgc tgctgatggc gggggtaagt cacgacttgc gcacgccgct 4561 gacgcgtatt cgcctggcga ctgagatgat gagcgagcag gatggctatc tggcagaatc 4621 gatcaataaa gatatcgaag agtgcaacgc catcattgag cagtttatcg actacctgcg 4681 caccgggcag gagatgccga tggaaatggc ggatcttaat gcagtactcg gtgaggtgat 4741 tgctgccgaa agtggctatg agcgggaaat tgaaaccgcg ctttaccccg gcagcattga 4801 agtgaaaatg cacccgctgt cgatcaaacg cgcggtggcg aatatggtgg tcaacgccgc 4861 ccgttatggc aatggctggg tcaaagtcag cagcggaacg gagccgaatc gcgcctggtt 4921 ccaggtggaa gatgacggtc cgggaattgc gccggaacaa cgtaagcacc tgttccagcc 4981 gtttgtccgc ggcgacagtg cgcgcaccat tagcggcacg ggattagggc tggcaattgt 5041 gcagcgtatc gtggataacc ataacgggat gctggagctt ggcaccagcg agcggggcgg 5101 gctttccatt cgcgcctggc tgccagtgcc ggtaacgcgg gcgcagggca tgacaaaaga 5161 agggtaataa ctctgatagt gctagtgtag atctctacta gagccaggca tcaaataaaa 5221 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 5281 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //