LOCUS BBa_I737012 2775 bp DNA linear 22-Nov-2024 DEFINITION With AHL, light -> area of no GFP. ACCESSION BBa_I737012 VERSION BBa_I737012.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 37..44 /label=linker promoter 45..50 /label=-35 promoter 68..73 /label=-10 misc_feature 41..41 /label=A promoter 1..78 /label=BBa_R0083 misc_feature 91..94 /label=conserved RBS 87..98 /label=BBa_B0034 misc_feature 105..106 /label=prefix misc_feature 278..278 /label=T misc_feature 596..596 /label=A CDS 105..854 /label=luxR misc_feature 105..860 /label=BBa_C0062 misc_feature 861..885 /label=Help:Barcodes CDS 105..860 /label=BBa_C0062 stem_loop 905..948 /label=stem_loop misc_feature 894..973 /label=BBa_B0010 terminator 894..973 /label=BBa_B0010 stem_loop 989..1008 /label=T7 TE misc_feature 1015..1015 /label=stop polyA_site 1009..1022 /label=polya misc_feature 982..1022 /label=BBa_B0012 terminator 982..1022 /label=BBa_B0012 misc_feature 1031..1050 /label=LuxR/HSL promoter 1050..1055 /label=-35 promoter 1072..1077 /label=-10 misc_feature 1083..1083 /label=start misc_feature 1031..1085 /label=BBa_R0062 promoter 1031..1085 /label=BBa_R0062 misc_feature 87..1085 /label=BBa_A340620 misc_feature 1098..1101 /label=conserved RBS 1094..1105 /label=BBa_B0034 misc_feature 1112..1113 /label=prefix misc_feature 1285..1285 /label=T misc_feature 1603..1603 /label=A CDS 1112..1861 /label=luxR misc_feature 1112..1867 /label=BBa_C0062 misc_feature 1868..1892 /label=Help:Barcodes CDS 1112..1867 /label=BBa_C0062 misc_feature 1094..1892 /label=BBa_S01414 misc_feature 1905..1908 /label=conserved RBS 1901..1912 /label=BBa_B0034 CDS 1919..2638 /label=GFP protein CDS 1919..2638 /label=BBa_E0040 stem_loop 2658..2701 /label=stem_loop misc_feature 2647..2726 /label=BBa_B0010 terminator 2647..2726 /label=BBa_B0010 stem_loop 2742..2761 /label=T7 TE misc_feature 2768..2768 /label=stop polyA_site 2762..2775 /label=polya misc_feature 2735..2775 /label=BBa_B0012 terminator 2735..2775 /label=BBa_B0012 misc_feature 1901..2775 /label=BBa_I13504 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat 61 ttttggggag aatggactta ctagagaaag aggagaaata ctagatgaaa aacataaatg 121 ccgacgacac atacagaata attaataaaa ttaaagcttg tagaagcaat aatgatatta 181 atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta ctcgcgatca 241 tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac cctaaaaaat 301 ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta gattattcta 361 actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta aataaaaaat 421 ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt agtttcccta 481 ttcatacggc taacaatggc ttcggaatgc ttagttttgc acattcagaa aaagacaact 541 atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt ccttctctag 601 ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat ttaaccaaaa 661 gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat atttcaaaaa 721 tattaggttg cagtgagcgt actgtcactt tccatttaac caatgcgcaa atgaaactca 781 atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca attgattgcc 841 catactttaa aaattaataa cactgatagt gctagtgtag atcactacta gagccaggca 901 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 961 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 1021 tatactagag acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga 1081 ataaatacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata 1141 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc 1201 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc 1261 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta 1321 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc 1381 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat 1441 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa 1501 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt 1561 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg 1621 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg 1681 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag 1741 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg 1801 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa 1861 ttaataacac tgatagtgct agtgtagatc actactagag aaagaggaga aatactagat 1921 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga 1981 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa 2041 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt 2101 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca 2161 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa 2221 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa 2281 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt 2341 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat 2401 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca 2461 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 2521 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct 2581 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata 2641 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2701 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2761 ctttctgcgt ttata //