##gff-version 3
##sequence-region BBa_I737012 1 2775
BBa_I737012	.	engineered_region	1901	2775	.	+	0	ID=BBa_I13504;Name=BBa_I13504
BBa_I737012	.	ribosome_entry_site	1901	1912	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13504
BBa_I737012	.	conserved_region	1905	1908	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737012	.	terminator	2735	2775	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13504
BBa_I737012	.	stem_loop	2742	2761	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737012	.	polyA_site	2762	2775	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737012	.	stop_codon	2768	2768	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737012	.	engineered_region	2735	2775	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737012	.	terminator	2647	2726	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13504
BBa_I737012	.	stem_loop	2658	2701	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737012	.	engineered_region	2647	2726	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737012	.	CDS	1919	2638	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_I13504
BBa_I737012	.	CDS	1919	2638	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I737012	.	promoter	1	78	.	+	0	ID=BBa_R0083;Name=BBa_R0083
BBa_I737012	.	sequence_feature	37	44	.	+	0	ID=annotation301223;Name=linker;Parent=BBa_R0083
BBa_I737012	.	promoter	68	73	.	+	0	ID=annotation301226;Name=-10;Parent=BBa_R0083
BBa_I737012	.	promoter	45	50	.	+	0	ID=annotation301224;Name=-35;Parent=BBa_R0083
BBa_I737012	.	point_mutation	41	41	.	+	0	ID=annotation306881;Name=A;Parent=BBa_R0083
BBa_I737012	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301221;Name=C1 OmpR;Parent=BBa_R0083
BBa_I737012	.	engineered_region	87	1085	.	+	0	ID=BBa_A340620;Name=BBa_A340620
BBa_I737012	.	ribosome_entry_site	87	98	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_A340620
BBa_I737012	.	conserved_region	91	94	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737012	.	terminator	894	973	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_A340620
BBa_I737012	.	stem_loop	905	948	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I737012	.	engineered_region	894	973	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I737012	.	terminator	982	1022	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_A340620
BBa_I737012	.	stem_loop	989	1008	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I737012	.	polyA_site	1009	1022	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I737012	.	stop_codon	1015	1015	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I737012	.	engineered_region	982	1022	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I737012	.	CDS	105	860	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_A340620
BBa_I737012	.	engineered_region	105	106	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I737012	.	engineered_tag	861	885	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I737012	.	CDS	105	854	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I737012	.	point_mutation	596	596	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I737012	.	point_mutation	278	278	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I737012	.	engineered_region	105	860	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I737012	.	promoter	1031	1085	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_A340620
BBa_I737012	.	promoter	1050	1055	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_I737012	.	engineered_region	1031	1085	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_I737012	.	non_covalent_binding_site	1031	1050	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_I737012	.	start_codon	1083	1083	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_I737012	.	promoter	1072	1077	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_I737012	.	engineered_region	1094	1892	.	+	0	ID=BBa_S01414;Name=BBa_S01414
BBa_I737012	.	ribosome_entry_site	1094	1105	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01414
BBa_I737012	.	conserved_region	1098	1101	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I737012	.	CDS	1112	1867	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01414
BBa_I737012	.	engineered_region	1112	1113	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I737012	.	engineered_tag	1868	1892	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I737012	.	CDS	1112	1861	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I737012	.	point_mutation	1603	1603	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I737012	.	point_mutation	1285	1285	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I737012	.	engineered_region	1112	1867	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
>BBa_I737012
tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggactt
actagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagct
tgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcga
tcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatga
tgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatattt
gaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtt
tccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagttt
atttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaat
aataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggata
tttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaa
ccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatag
tgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttat
ctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatacta
gagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaat
actagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatat
taatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatg
gttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaat
atgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataa
aaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaac
aatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatga
acataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgattt
aaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgc
agtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttcta
aagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactact
agagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagat
ggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaat
ttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgc
gagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactata
tttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagt
taaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtata
catcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaa
ctagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacac
aatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattac
acatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata