LOCUS BBa_I737014 2805 bp DNA linear 26-Dec-2024 DEFINITION With AHL, light -> no GFP ACCESSION BBa_I737014 VERSION BBa_I737014.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 misc_feature 135..136 /label=prefix misc_feature 308..308 /label=T misc_feature 626..626 /label=A CDS 135..884 /label=luxR misc_feature 135..890 /label=BBa_C0062 misc_feature 891..915 /label=Help:Barcodes CDS 135..890 /label=BBa_C0062 stem_loop 935..978 /label=stem_loop misc_feature 924..1003 /label=BBa_B0010 terminator 924..1003 /label=BBa_B0010 stem_loop 1019..1038 /label=T7 TE misc_feature 1045..1045 /label=stop polyA_site 1039..1052 /label=polya misc_feature 1012..1052 /label=BBa_B0012 terminator 1012..1052 /label=BBa_B0012 misc_feature 1061..1080 /label=LuxR/HSL promoter 1080..1085 /label=-35 promoter 1102..1107 /label=-10 misc_feature 1113..1113 /label=start misc_feature 1061..1115 /label=BBa_R0062 promoter 1061..1115 /label=BBa_R0062 misc_feature 117..1115 /label=BBa_A340620 misc_feature 1128..1131 /label=conserved RBS 1124..1135 /label=BBa_B0034 misc_feature 1142..1143 /label=prefix misc_feature 1315..1315 /label=T misc_feature 1633..1633 /label=A CDS 1142..1891 /label=luxR misc_feature 1142..1897 /label=BBa_C0062 misc_feature 1898..1922 /label=Help:Barcodes CDS 1142..1897 /label=BBa_C0062 misc_feature 1124..1922 /label=BBa_S01414 misc_feature 1935..1938 /label=conserved RBS 1931..1942 /label=BBa_B0034 CDS 1949..2668 /label=GFP protein CDS 1949..2668 /label=BBa_E0040 stem_loop 2688..2731 /label=stem_loop misc_feature 2677..2756 /label=BBa_B0010 terminator 2677..2756 /label=BBa_B0010 stem_loop 2772..2791 /label=T7 TE misc_feature 2798..2798 /label=stop polyA_site 2792..2805 /label=polya misc_feature 2765..2805 /label=BBa_B0012 terminator 2765..2805 /label=BBa_B0012 misc_feature 1931..2805 /label=BBa_I13504 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa 181 ttaaagcttg tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg 241 tacattgtga atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata 301 tttcaatcct agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa 361 taaaatatga tcctatagta gattattcta actccaatca ttcaccaatt aattggaata 421 tatttgaaaa caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat 481 caggtcttat cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc 541 ttagttttgc acattcagaa aaagacaact atatagatag tttattttta catgcgtgta 601 tgaacatacc attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa 661 ataataaatc aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg 721 aaggaaaaag ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt 781 tccatttaac caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta 841 aagcaatttt aacaggagca attgattgcc catactttaa aaattaataa cactgatagt 901 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 961 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 1021 ctcaccttcg ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt 1081 ttacgcaaga aaatggtttg ttatagtcga ataaatacta gagaaagagg agaaatacta 1141 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 1201 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 1261 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 1321 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 1381 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 1441 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 1501 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 1561 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 1621 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 1681 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 1741 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 1801 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 1861 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 1921 actactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt 1981 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg 2041 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg 2101 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt 2161 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg 2221 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga 2281 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa 2341 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata 2401 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat 2461 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg 2521 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc 2581 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca 2641 tggcatggat gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg 2701 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2761 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //