BBa_I737020 1 BBa_I737020 PoPS->Agarase (Digests agar) 2007-10-25T11:00:00Z 2015-08-31T04:08:01Z Cloned out of Pseudoalteromonas atlantica T6C from the ATCC. This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. Currently the gene has a EcorRI site which limits how it can be assembled. false false _114_ 0 905 77 It's complicated true There is a EcoRI site in the sequence which must be mutated at some point. false Vladislav Lavrovsky annotation1957377 1 Reverse Primer range1957377 1 2571 2591 annotation1957376 1 Forward Primer range1957376 1 1 20 annotation1957509 1 Stop range1957509 1 2493 2495 annotation1955819 1 Agarase range1955819 1 53 2495 annotation1957508 1 Start range1957508 1 53 56 BBa_I737020_sequence 1 tatccgcacatacccaaggttgagcaccctatttaataacggaaacaacaacatgaaatacccacaattcattacaaaggtcgcctttagggcaagcgcgattgcgctagctattaccctaaccggctgtagtcatgatcaacatgaaattgctcaggctcagggtgttccacaagaccttgctggaaacggtagcccactaggcagtgcagaggacgcgtttaccaacgagcaaaaaatcgtctcactgattgactttgataaccaagagcaacttaactggctgcagcaaagtgatacctcattcgcgcagatcaccagcagcgagagcttaattgacaagcaattagcggtggagtttgcgaaaaccggtaatatttccacgttgcgcgtagagccaccgaagccttgggatcttagggagtacgaaaactacaacattgcctttgatgtgcaaaacaccagcccagattcagttcatttgtacttgtctttggaaaatgtaaacggacaaatccagagtcactcaatttctctggctccgaattacaaaggcacggtgtattttcccctcgatggtattgaagcagaaaccgacagcggcatgtggggagatgtgcctcattggcaaacccaagacgacttaatggtgtggcgttcttggcgcaaagcagagcaagactatagccaactcaccgcgcttaatttcttcactattggaatattggaaaacaaatctgtgattttggatgatgtgcgtctgcgcgcgaatccagctcacgacccgaacgtatcggtcggattaatcgataaatacgggcaaaacgccaaacaaagcacccccttagatgtgcattcagatgcccaactaaaacaacaagccgatgaagaactggccaagctagccaaatcatcgggtatgccagaccgttctcgttttggtggttataccaaagccccaaaacgtgaagcaaccggcttttttagaacggaaaaggtcgatggtaagtggtggatggtcgaccctgatggctatctgtttttctcacacgggccagctaacgtgcgtatggcaaacatgagtacgttaaccggaatagactacgaccaaccgagtattcgcgtgcgcaccagtgatgaaattaccccagaagattccatgggaattgtatctgttcctgacagcgcaaaagaaaaacgctatgtcatttcaaaggcgcgcaacgatatgtttcagtggttaccaagctatgatgatgagctctctgagcactatagctatcgccgctctacccacaaagggcctattccttacggcgagacctacagcttttaccgcgctaatttagaacgtagatacggtgatgacgacctacgcgcaggtgaaggtgaaaaagcaggcgaaaatcaaggcagcgatccttcctacgtaaaaaaatggcatgacgtcaccgcacagcgtatgcatgactgggggtttacctcttttggtaactgggtagacccagcgttttatcaatcagagcaagtgccttatttcgccaatggttggatcatcggcgattaccaaacactgtcgggtcacactaatcattggggcttgatgccagatccttttgatcccgtatttgcacagcgagcaaaggtcactatcgatgctattgccgaaaatattcaagcgtcaccttggtgtgcgggtatttttattgataatgagaagagctggggagagcgtgaaggcacagtgtcacaacgctatggggtgatccttgatgcactatcaaaaaatattgagaagagccctgcaaaaacggcctttgcagcccatttgcagcaaaaataccagacgatcgatgcattgaattctgcatggtcatcacacttcgatagttggcaaatgtttgaagatgacgccagcgtcagcacgcacaccgatgcccaaattgctgatttgtcaaaaatgctcgagatgctgggtgagcagtactttaaagtggtacacaataccttagaagcggcgctgccagagcacttgtacatgggcgcgcgcatggccaactggggcatgcctgatgaaatcattactgcctctttgaaatactctgacgtactgagcttcaatatttatgaggaaggggtgcaagaagactattggaagttccttgaagaagtcgacttacccgtagtgattggtgagtttcatattggcagcacgacagattcaggcatgtacaacccgggtattgtgcacagcgcaaaccagcaagacagagcccaaatgtacaaagattacatgcaaagcgtgttagacaagccgtatatggtcggggcccattggtttcaatacatagatgagcccatatcggggcgtgcatttgacggcgaaaacgccaatatcggctttgtaacggtaacggatatcccatatccgcatatgatccaagcggtcaaagaggtgacatccaccatgtaccaaaaacgcttagccgactaatacaggcttagtgataacaaaaaacgcccgataacgtcgggcgttttgcgtttgtaaacgactattctccttgcacgttaactaccactcgaccgcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z