LOCUS       BBa_I737021             2673 bp    DNA     linear       25-Nov-2024
DEFINITION  Constitutively expressed Agarase part.
ACCESSION   BBa_I737021
VERSION     BBa_I737021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             135..2577
                     /label=Agarase
     primer_bind     83..102
                     /label=Forward Primer
     primer_bind     2653..2673
                     /label=Reverse Primer
     misc_feature    135..138
                     /label=Start
     misc_feature    2575..2577
                     /label=Stop
     misc_feature    83..2673
                     /label=BBa_I737020
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agtatccgca catacccaag gttgagcacc ctatttaata
      121 acggaaacaa caacatgaaa tacccacaat tcattacaaa ggtcgccttt agggcaagcg
      181 cgattgcgct agctattacc ctaaccggct gtagtcatga tcaacatgaa attgctcagg
      241 ctcagggtgt tccacaagac cttgctggaa acggtagccc actaggcagt gcagaggacg
      301 cgtttaccaa cgagcaaaaa atcgtctcac tgattgactt tgataaccaa gagcaactta
      361 actggctgca gcaaagtgat acctcattcg cgcagatcac cagcagcgag agcttaattg
      421 acaagcaatt agcggtggag tttgcgaaaa ccggtaatat ttccacgttg cgcgtagagc
      481 caccgaagcc ttgggatctt agggagtacg aaaactacaa cattgccttt gatgtgcaaa
      541 acaccagccc agattcagtt catttgtact tgtctttgga aaatgtaaac ggacaaatcc
      601 agagtcactc aatttctctg gctccgaatt acaaaggcac ggtgtatttt cccctcgatg
      661 gtattgaagc agaaaccgac agcggcatgt ggggagatgt gcctcattgg caaacccaag
      721 acgacttaat ggtgtggcgt tcttggcgca aagcagagca agactatagc caactcaccg
      781 cgcttaattt cttcactatt ggaatattgg aaaacaaatc tgtgattttg gatgatgtgc
      841 gtctgcgcgc gaatccagct cacgacccga acgtatcggt cggattaatc gataaatacg
      901 ggcaaaacgc caaacaaagc acccccttag atgtgcattc agatgcccaa ctaaaacaac
      961 aagccgatga agaactggcc aagctagcca aatcatcggg tatgccagac cgttctcgtt
     1021 ttggtggtta taccaaagcc ccaaaacgtg aagcaaccgg cttttttaga acggaaaagg
     1081 tcgatggtaa gtggtggatg gtcgaccctg atggctatct gtttttctca cacgggccag
     1141 ctaacgtgcg tatggcaaac atgagtacgt taaccggaat agactacgac caaccgagta
     1201 ttcgcgtgcg caccagtgat gaaattaccc cagaagattc catgggaatt gtatctgttc
     1261 ctgacagcgc aaaagaaaaa cgctatgtca tttcaaaggc gcgcaacgat atgtttcagt
     1321 ggttaccaag ctatgatgat gagctctctg agcactatag ctatcgccgc tctacccaca
     1381 aagggcctat tccttacggc gagacctaca gcttttaccg cgctaattta gaacgtagat
     1441 acggtgatga cgacctacgc gcaggtgaag gtgaaaaagc aggcgaaaat caaggcagcg
     1501 atccttccta cgtaaaaaaa tggcatgacg tcaccgcaca gcgtatgcat gactgggggt
     1561 ttacctcttt tggtaactgg gtagacccag cgttttatca atcagagcaa gtgccttatt
     1621 tcgccaatgg ttggatcatc ggcgattacc aaacactgtc gggtcacact aatcattggg
     1681 gcttgatgcc agatcctttt gatcccgtat ttgcacagcg agcaaaggtc actatcgatg
     1741 ctattgccga aaatattcaa gcgtcacctt ggtgtgcggg tatttttatt gataatgaga
     1801 agagctgggg agagcgtgaa ggcacagtgt cacaacgcta tggggtgatc cttgatgcac
     1861 tatcaaaaaa tattgagaag agccctgcaa aaacggcctt tgcagcccat ttgcagcaaa
     1921 aataccagac gatcgatgca ttgaattctg catggtcatc acacttcgat agttggcaaa
     1981 tgtttgaaga tgacgccagc gtcagcacgc acaccgatgc ccaaattgct gatttgtcaa
     2041 aaatgctcga gatgctgggt gagcagtact ttaaagtggt acacaatacc ttagaagcgg
     2101 cgctgccaga gcacttgtac atgggcgcgc gcatggccaa ctggggcatg cctgatgaaa
     2161 tcattactgc ctctttgaaa tactctgacg tactgagctt caatatttat gaggaagggg
     2221 tgcaagaaga ctattggaag ttccttgaag aagtcgactt acccgtagtg attggtgagt
     2281 ttcatattgg cagcacgaca gattcaggca tgtacaaccc gggtattgtg cacagcgcaa
     2341 accagcaaga cagagcccaa atgtacaaag attacatgca aagcgtgtta gacaagccgt
     2401 atatggtcgg ggcccattgg tttcaataca tagatgagcc catatcgggg cgtgcatttg
     2461 acggcgaaaa cgccaatatc ggctttgtaa cggtaacgga tatcccatat ccgcatatga
     2521 tccaagcggt caaagaggtg acatccacca tgtaccaaaa acgcttagcc gactaataca
     2581 ggcttagtga taacaaaaaa cgcccgataa cgtcgggcgt tttgcgtttg taaacgacta
     2641 ttctccttgc acgttaacta ccactcgacc gcg
//