BBa_I737021 1 BBa_I737021 Constitutively expressed Agarase part. 2007-10-25T11:00:00Z 2015-08-31T04:08:01Z Cloned from Pseudoalteromonas atlantica T6C. This part constituatively expresses agarase. It is used to produce e. coli which degrade the agar substrate that they are normally grown on. Typical rate of degradation is low, it takes two to three days to degrade 4mm of agar underneath the colonies. false false _114_ 0 905 77 Not in stock false There are EcorI and PstI sites. This limits construction. Future versions will be mutated by site-directed mutagenesis. The flanks of the gene were included for the signal peptide. Agarase is exported in the source organism using the TAT export system with e. coli K12 has, unfortunately so far e. coli dont degrade agar when transformed with this part. We are not sure whether it is a problem with expression or export. false Vladislav Lavrovsky component1955838 1 BBa_R0040 component1955845 1 BBa_I737020 component1955843 1 BBa_B0034 annotation1955838 1 BBa_R0040 range1955838 1 1 54 annotation1955843 1 BBa_B0034 range1955843 1 63 74 annotation1955845 1 BBa_I737020 range1955845 1 83 2673 BBa_I737020 1 BBa_I737020 PoPS->Agarase (Digests agar) 2007-10-25T11:00:00Z 2015-08-31T04:08:01Z Cloned out of Pseudoalteromonas atlantica T6C from the ATCC. This part is Beta-Agarase cloned out of Pseudoalteromonas atlantica T6C from the ATCC. Pseudoalteromonas atlantica is a gram-negative, motile, chemoorganotroph which lives in sea water. We made a series of primers and cloned agarase using a TOPO cloning kit. Currently the gene has a EcorRI site which limits how it can be assembled. false false _114_ 0 905 77 It's complicated true There is a EcoRI site in the sequence which must be mutated at some point. false Vladislav Lavrovsky annotation1957509 1 Stop range1957509 1 2493 2495 annotation1957508 1 Start range1957508 1 53 56 annotation1957377 1 Reverse Primer range1957377 1 2571 2591 annotation1955819 1 Agarase range1955819 1 53 2495 annotation1957376 1 Forward Primer range1957376 1 1 20 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 BBa_I737020_sequence 1 tatccgcacatacccaaggttgagcaccctatttaataacggaaacaacaacatgaaatacccacaattcattacaaaggtcgcctttagggcaagcgcgattgcgctagctattaccctaaccggctgtagtcatgatcaacatgaaattgctcaggctcagggtgttccacaagaccttgctggaaacggtagcccactaggcagtgcagaggacgcgtttaccaacgagcaaaaaatcgtctcactgattgactttgataaccaagagcaacttaactggctgcagcaaagtgatacctcattcgcgcagatcaccagcagcgagagcttaattgacaagcaattagcggtggagtttgcgaaaaccggtaatatttccacgttgcgcgtagagccaccgaagccttgggatcttagggagtacgaaaactacaacattgcctttgatgtgcaaaacaccagcccagattcagttcatttgtacttgtctttggaaaatgtaaacggacaaatccagagtcactcaatttctctggctccgaattacaaaggcacggtgtattttcccctcgatggtattgaagcagaaaccgacagcggcatgtggggagatgtgcctcattggcaaacccaagacgacttaatggtgtggcgttcttggcgcaaagcagagcaagactatagccaactcaccgcgcttaatttcttcactattggaatattggaaaacaaatctgtgattttggatgatgtgcgtctgcgcgcgaatccagctcacgacccgaacgtatcggtcggattaatcgataaatacgggcaaaacgccaaacaaagcacccccttagatgtgcattcagatgcccaactaaaacaacaagccgatgaagaactggccaagctagccaaatcatcgggtatgccagaccgttctcgttttggtggttataccaaagccccaaaacgtgaagcaaccggcttttttagaacggaaaaggtcgatggtaagtggtggatggtcgaccctgatggctatctgtttttctcacacgggccagctaacgtgcgtatggcaaacatgagtacgttaaccggaatagactacgaccaaccgagtattcgcgtgcgcaccagtgatgaaattaccccagaagattccatgggaattgtatctgttcctgacagcgcaaaagaaaaacgctatgtcatttcaaaggcgcgcaacgatatgtttcagtggttaccaagctatgatgatgagctctctgagcactatagctatcgccgctctacccacaaagggcctattccttacggcgagacctacagcttttaccgcgctaatttagaacgtagatacggtgatgacgacctacgcgcaggtgaaggtgaaaaagcaggcgaaaatcaaggcagcgatccttcctacgtaaaaaaatggcatgacgtcaccgcacagcgtatgcatgactgggggtttacctcttttggtaactgggtagacccagcgttttatcaatcagagcaagtgccttatttcgccaatggttggatcatcggcgattaccaaacactgtcgggtcacactaatcattggggcttgatgccagatccttttgatcccgtatttgcacagcgagcaaaggtcactatcgatgctattgccgaaaatattcaagcgtcaccttggtgtgcgggtatttttattgataatgagaagagctggggagagcgtgaaggcacagtgtcacaacgctatggggtgatccttgatgcactatcaaaaaatattgagaagagccctgcaaaaacggcctttgcagcccatttgcagcaaaaataccagacgatcgatgcattgaattctgcatggtcatcacacttcgatagttggcaaatgtttgaagatgacgccagcgtcagcacgcacaccgatgcccaaattgctgatttgtcaaaaatgctcgagatgctgggtgagcagtactttaaagtggtacacaataccttagaagcggcgctgccagagcacttgtacatgggcgcgcgcatggccaactggggcatgcctgatgaaatcattactgcctctttgaaatactctgacgtactgagcttcaatatttatgaggaaggggtgcaagaagactattggaagttccttgaagaagtcgacttacccgtagtgattggtgagtttcatattggcagcacgacagattcaggcatgtacaacccgggtattgtgcacagcgcaaaccagcaagacagagcccaaatgtacaaagattacatgcaaagcgtgttagacaagccgtatatggtcggggcccattggtttcaatacatagatgagcccatatcggggcgtgcatttgacggcgaaaacgccaatatcggctttgtaacggtaacggatatcccatatccgcatatgatccaagcggtcaaagaggtgacatccaccatgtaccaaaaacgcttagccgactaatacaggcttagtgataacaaaaaacgcccgataacgtcgggcgttttgcgtttgtaaacgactattctccttgcacgttaactaccactcgaccgcg BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I737021_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagagtatccgcacatacccaaggttgagcaccctatttaataacggaaacaacaacatgaaatacccacaattcattacaaaggtcgcctttagggcaagcgcgattgcgctagctattaccctaaccggctgtagtcatgatcaacatgaaattgctcaggctcagggtgttccacaagaccttgctggaaacggtagcccactaggcagtgcagaggacgcgtttaccaacgagcaaaaaatcgtctcactgattgactttgataaccaagagcaacttaactggctgcagcaaagtgatacctcattcgcgcagatcaccagcagcgagagcttaattgacaagcaattagcggtggagtttgcgaaaaccggtaatatttccacgttgcgcgtagagccaccgaagccttgggatcttagggagtacgaaaactacaacattgcctttgatgtgcaaaacaccagcccagattcagttcatttgtacttgtctttggaaaatgtaaacggacaaatccagagtcactcaatttctctggctccgaattacaaaggcacggtgtattttcccctcgatggtattgaagcagaaaccgacagcggcatgtggggagatgtgcctcattggcaaacccaagacgacttaatggtgtggcgttcttggcgcaaagcagagcaagactatagccaactcaccgcgcttaatttcttcactattggaatattggaaaacaaatctgtgattttggatgatgtgcgtctgcgcgcgaatccagctcacgacccgaacgtatcggtcggattaatcgataaatacgggcaaaacgccaaacaaagcacccccttagatgtgcattcagatgcccaactaaaacaacaagccgatgaagaactggccaagctagccaaatcatcgggtatgccagaccgttctcgttttggtggttataccaaagccccaaaacgtgaagcaaccggcttttttagaacggaaaaggtcgatggtaagtggtggatggtcgaccctgatggctatctgtttttctcacacgggccagctaacgtgcgtatggcaaacatgagtacgttaaccggaatagactacgaccaaccgagtattcgcgtgcgcaccagtgatgaaattaccccagaagattccatgggaattgtatctgttcctgacagcgcaaaagaaaaacgctatgtcatttcaaaggcgcgcaacgatatgtttcagtggttaccaagctatgatgatgagctctctgagcactatagctatcgccgctctacccacaaagggcctattccttacggcgagacctacagcttttaccgcgctaatttagaacgtagatacggtgatgacgacctacgcgcaggtgaaggtgaaaaagcaggcgaaaatcaaggcagcgatccttcctacgtaaaaaaatggcatgacgtcaccgcacagcgtatgcatgactgggggtttacctcttttggtaactgggtagacccagcgttttatcaatcagagcaagtgccttatttcgccaatggttggatcatcggcgattaccaaacactgtcgggtcacactaatcattggggcttgatgccagatccttttgatcccgtatttgcacagcgagcaaaggtcactatcgatgctattgccgaaaatattcaagcgtcaccttggtgtgcgggtatttttattgataatgagaagagctggggagagcgtgaaggcacagtgtcacaacgctatggggtgatccttgatgcactatcaaaaaatattgagaagagccctgcaaaaacggcctttgcagcccatttgcagcaaaaataccagacgatcgatgcattgaattctgcatggtcatcacacttcgatagttggcaaatgtttgaagatgacgccagcgtcagcacgcacaccgatgcccaaattgctgatttgtcaaaaatgctcgagatgctgggtgagcagtactttaaagtggtacacaataccttagaagcggcgctgccagagcacttgtacatgggcgcgcgcatggccaactggggcatgcctgatgaaatcattactgcctctttgaaatactctgacgtactgagcttcaatatttatgaggaaggggtgcaagaagactattggaagttccttgaagaagtcgacttacccgtagtgattggtgagtttcatattggcagcacgacagattcaggcatgtacaacccgggtattgtgcacagcgcaaaccagcaagacagagcccaaatgtacaaagattacatgcaaagcgtgttagacaagccgtatatggtcggggcccattggtttcaatacatagatgagcccatatcggggcgtgcatttgacggcgaaaacgccaatatcggctttgtaacggtaacggatatcccatatccgcatatgatccaagcggtcaaagaggtgacatccaccatgtaccaaaaacgcttagccgactaatacaggcttagtgataacaaaaaacgcccgataacgtcgggcgttttgcgtttgtaaacgactattctccttgcacgttaactaccactcgaccgcg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z