LOCUS       BBa_I739005              917 bp    DNA     linear       25-Nov-2024
DEFINITION  Promoterless expression cassette for EYFP +LVA (B0034.E0032.B0015)
ACCESSION   BBa_I739005
VERSION     BBa_I739005.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..780
                     /label=YFP (LVA)
     misc_feature    775..780
                     /label=2
     misc_feature    736..774
                     /label=SsrA
     misc_feature    260..260
                     /label=A (Q->L)
     misc_feature    19..780
                     /label=BBa_E0032
     misc_feature    19..780
                     /label=BBa_E0032
     stem_loop       800..843
                     /label=stem_loop
     misc_feature    789..868
                     /label=BBa_B0010
     terminator      789..868
                     /label=BBa_B0010
     stem_loop       884..903
                     /label=T7 TE
     misc_feature    910..910
                     /label=stop
     polyA_site      904..917
                     /label=polya
     misc_feature    877..917
                     /label=BBa_B0012
     terminator      877..917
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
       61 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      121 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      181 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
      241 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      301 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      361 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      421 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
      481 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
      541 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      601 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
      661 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      721 gacgagctgt acaagaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      781 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      841 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      901 gcctttctgc gtttata
//