LOCUS       BBa_I739010              967 bp    DNA     linear       22-Nov-2024
DEFINITION  Double regulated expression cassette for RFP +LVA 
            (I739106.B0034.J04051.B0015)
ACCESSION   BBa_I739010
VERSION     BBa_I739010.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=R0040
     misc_feature    49..66
                     /label=OR1
     misc_feature    67..84
                     /label=OR1
     misc_feature    49..84
                     /label=R0053 (2x OR1)
     promoter        1..84
                     /label=BBa_I739106
     misc_feature    97..100
                     /label=conserved
     RBS             93..104
                     /label=BBa_B0034
     CDS             111..785
                     /label=mrfp1
     misc_feature    825..830
                     /label=Stop
     misc_feature    786..824
                     /label=LVA
     misc_feature    111..113
                     /label=Start
     misc_feature    111..830
                     /label=BBa_J04051
     stem_loop       850..893
                     /label=stem_loop
     misc_feature    839..918
                     /label=BBa_B0010
     terminator      839..918
                     /label=BBa_B0010
     stem_loop       934..953
                     /label=T7 TE
     misc_feature    960..960
                     /label=stop
     polyA_site      954..967
                     /label=polya
     misc_feature    927..967
                     /label=BBa_B0012
     terminator      927..967
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactat ttaagtgttc
       61 tttaatattt aagtgttctt taattactag agaaagagga gaaatactag atggcttcct
      121 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
      181 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
      241 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
      301 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
      361 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
      421 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
      481 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
      541 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
      601 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
      661 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
      721 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
      781 gtgctaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa tactagagcc
      841 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      901 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      961 gtttata
//