LOCUS       BBa_I739011             1000 bp    DNA     linear       22-Dec-2024
DEFINITION  Double regulated expression cassette for GFP +LVA 
            (I739107.B0034.J04031.B0015)
ACCESSION   BBa_I739011
VERSION     BBa_I739011.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=R0051
     misc_feature    44..78
                     /label=LacI binding site
     misc_feature    44..78
                     /label=R0010
     promoter        1..78
                     /label=BBa_I739107
     misc_feature    91..94
                     /label=conserved
     RBS             87..98
                     /label=BBa_B0034
     misc_feature    105..824
                     /label=BBa_E0040
     misc_feature    819..857
                     /label=LVA
     misc_feature    858..863
                     /label=Double Stop
     misc_feature    105..107
                     /label=Start
     misc_feature    105..863
                     /label=BBa_J04031
     stem_loop       883..926
                     /label=stem_loop
     misc_feature    872..951
                     /label=BBa_B0010
     terminator      872..951
                     /label=BBa_B0010
     stem_loop       967..986
                     /label=T7 TE
     misc_feature    993..993
                     /label=stop
     polyA_site      987..1000
                     /label=polya
     misc_feature    960..1000
                     /label=BBa_B0012
     terminator      960..1000
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aattgtgtgg aattgtgagc
       61 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatgcgt aaaggagaag
      121 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      181 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      241 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      301 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      361 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      421 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      481 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      541 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      601 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      661 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      721 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      781 ctgctgggat tacacatggc atggatgaac tatacaaaag gcctgctgca aacgacgaaa
      841 actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag
      901 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      961 cacactggct caccttcggg tgggcctttc tgcgtttata
//