LOCUS BBa_I739013 3229 bp DNA linear 22-Nov-2024 DEFINITION Composite of BBa_I739001, BBa_I739002 and BBa_I739003 ACCESSION BBa_I739013 VERSION BBa_I739013.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23105 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_I739001 promoter 892..926 /label=BBa_J23105 misc_feature 939..942 /label=conserved RBS 935..946 /label=BBa_B0034 misc_feature 2042..2080 /label=LVA CDS 953..2080 /label=lacI-LVA misc_feature 953..2080 /label=BBa_C0012 misc_feature 2081..2105 /label=Help:Barcodes CDS 953..2080 /label=BBa_C0012 stem_loop 2125..2168 /label=stem_loop misc_feature 2114..2193 /label=BBa_B0010 terminator 2114..2193 /label=BBa_B0010 stem_loop 2209..2228 /label=T7 TE misc_feature 2235..2235 /label=stop polyA_site 2229..2242 /label=polya misc_feature 2202..2242 /label=BBa_B0012 terminator 2202..2242 /label=BBa_B0012 terminator 2114..2242 /label=BBa_B0015 misc_feature 892..2242 /label=BBa_I739002 promoter 2251..2285 /label=BBa_J23105 misc_feature 2298..2301 /label=conserved RBS 2294..2305 /label=BBa_B0034 misc_feature 2312..2313 /label=prefix misc_feature 2485..2485 /label=T misc_feature 2803..2803 /label=A CDS 2312..3061 /label=luxR misc_feature 2312..3067 /label=BBa_C0062 misc_feature 3068..3092 /label=Help:Barcodes CDS 2312..3067 /label=BBa_C0062 stem_loop 3112..3155 /label=stem_loop misc_feature 3101..3180 /label=BBa_B0010 terminator 3101..3180 /label=BBa_B0010 stem_loop 3196..3215 /label=T7 TE misc_feature 3222..3222 /label=stop polyA_site 3216..3229 /label=polya misc_feature 3189..3229 /label=BBa_B0012 terminator 3189..3229 /label=BBa_B0012 terminator 3101..3229 /label=BBa_B0015 misc_feature 2251..3229 /label=BBa_I739003 ORIGIN 1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtttacggct 901 agctcagtcc taggtactat gctagctact agagaaagag gagaaatact agatggtgaa 961 tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt 1021 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc 1081 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca 1141 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt 1201 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga 1261 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag 1321 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg 1381 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat 1441 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca 1501 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg 1561 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg 1621 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac 1681 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc 1741 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc 1801 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag 1861 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc 1921 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg 1981 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 2041 ggctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag 2101 atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 2161 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 2221 ggtgggcctt tctgcgttta tatactagag tttacggcta gctcagtcct aggtactatg 2281 ctagctacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata 2341 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc 2401 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc 2461 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta 2521 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc 2581 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat 2641 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa 2701 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt 2761 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg 2821 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg 2881 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag 2941 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg 3001 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa 3061 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga 3121 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 3181 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //