##gff-version 3
##sequence-region BBa_I739013 1 3229
BBa_I739013	.	engineered_region	2251	3229	.	+	0	ID=BBa_I739003;Name=BBa_I739003
BBa_I739013	.	CDS	2312	3067	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I739003
BBa_I739013	.	engineered_region	2312	2313	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I739013	.	CDS	2312	3061	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I739013	.	point_mutation	2485	2485	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I739013	.	engineered_tag	3068	3092	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I739013	.	point_mutation	2803	2803	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I739013	.	engineered_region	2312	3067	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I739013	.	promoter	2251	2285	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739003
BBa_I739013	.	terminator	3101	3229	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739003
BBa_I739013	.	terminator	3189	3229	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I739013	.	engineered_region	3189	3229	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739013	.	polyA_site	3216	3229	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739013	.	stop_codon	3222	3222	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739013	.	stem_loop	3196	3215	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739013	.	terminator	3101	3180	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I739013	.	engineered_region	3101	3180	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739013	.	stem_loop	3112	3155	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739013	.	ribosome_entry_site	2294	2305	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739003
BBa_I739013	.	conserved_region	2298	2301	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739013	.	engineered_region	1	883	.	+	0	ID=BBa_I739001;Name=BBa_I739001
BBa_I739013	.	promoter	1	35	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739001
BBa_I739013	.	CDS	62	746	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_I739001
BBa_I739013	.	tag	682	715	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I739013	.	engineered_tag	722	746	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I739013	.	CDS	65	681	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I739013	.	terminator	755	883	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739001
BBa_I739013	.	terminator	843	883	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I739013	.	engineered_region	843	883	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739013	.	polyA_site	870	883	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739013	.	stop_codon	876	876	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739013	.	stem_loop	850	869	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739013	.	terminator	755	834	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I739013	.	engineered_region	755	834	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739013	.	stem_loop	766	809	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739013	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739001
BBa_I739013	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739013	.	engineered_region	892	2242	.	+	0	ID=BBa_I739002;Name=BBa_I739002
BBa_I739013	.	ribosome_entry_site	935	946	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I739002
BBa_I739013	.	conserved_region	939	942	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I739013	.	terminator	2114	2242	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_I739002
BBa_I739013	.	terminator	2202	2242	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_I739013	.	engineered_region	2202	2242	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I739013	.	polyA_site	2229	2242	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I739013	.	stop_codon	2235	2235	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I739013	.	stem_loop	2209	2228	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I739013	.	terminator	2114	2193	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_I739013	.	engineered_region	2114	2193	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I739013	.	stem_loop	2125	2168	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I739013	.	promoter	892	926	.	+	0	ID=BBa_J23105;Name=BBa_J23105;Parent=BBa_I739002
BBa_I739013	.	CDS	953	2080	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_I739002
BBa_I739013	.	engineered_tag	2081	2105	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I739013	.	engineered_region	953	2080	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I739013	.	CDS	953	2080	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I739013	.	tag	2042	2080	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
>BBa_I739013
tttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaatactagatgtccagattagataaa
agtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccaga
agctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagat
gttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagt
tttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaa
ctctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggg
gcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgat
agtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttg
aattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagt
agcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagagtttacggctagctcagtcctaggtactatgctagctactagagaaagaggagaaata
ctagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgc
gtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattc
ccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgc
gccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacga
agcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgc
tggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagac
acccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccag
caaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactc
gcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgct
gaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgag
tccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgt
taaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggc
ggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctct
ccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacg
aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagtcctaggtactatgctagctactag
agaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtag
aagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatt
tatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacg
ctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaa
caatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccct
attcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttt
tacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataa
atcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttca
aaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgct
gccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgcta
gtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata