LOCUS       BBa_I739015             1721 bp    DNA     linear       22-Dec-2024
DEFINITION  Double regulated polycistronic P22 cII +LVA / EYFP -LVA expression 
            cassette
ACCESSION   BBa_I739015
VERSION     BBa_I739015.1  
FEATURES             Location/Qualifiers
     promoter        1..49
                     /label=R0051
     misc_feature    50..57
                     /label=Scar
     misc_feature    78..97
                     /label=TetR 1
     misc_feature    58..97
                     /label=R0040 (2x TetR 1)
     misc_feature    58..77
                     /label=TetR 1
     promoter        1..97
                     /label=BBa_I739102
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             124..771
                     /label=cII p22
     misc_feature    772..804
                     /label=LVA
     misc_feature    805..810
                     /label=stop
     misc_feature    124..810
                     /label=BBa_C0053
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             124..810
                     /label=BBa_C0053
     misc_feature    1..835
                     /label=BBa_I739004
     misc_feature    848..851
                     /label=conserved
     RBS             844..855
                     /label=BBa_B0034
     CDS             862..1584
                     /label=BBa_E0030
     stem_loop       1604..1647
                     /label=stem_loop
     misc_feature    1593..1672
                     /label=BBa_B0010
     terminator      1593..1672
                     /label=BBa_B0010
     stem_loop       1688..1707
                     /label=T7 TE
     misc_feature    1714..1714
                     /label=stop
     polyA_site      1708..1721
                     /label=polya
     misc_feature    1681..1721
                     /label=BBa_B0012
     terminator      1681..1721
                     /label=BBa_B0012
     misc_feature    844..1721
                     /label=BBa_E0430
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtcc
       61 ctatcagtga tagagattcc ctatcagtga tagagattac tagagaaaga ggagaaatac
      121 tagatgaata cacaattgat gggtgagcgt attcgcgctc gaagaaaaaa actcaagatt
      181 agacaagccg ctcttggtaa gatggtggga gtgtctaatg ttgcaatatc gcaatgggag
      241 cgctcggaga ctgagccaaa tggggagaac ctgttggcac tttcgaaggc tcttcagtgc
      301 tcccctgact atttgctgaa aggagattta agccagacaa acgttgccta tcatagtagg
      361 catgagccaa gaggatcata ccctcttatc agttgggtaa gcgcagggca atggatggaa
      421 gctgtagaac cttatcacaa gcgcgcgata gagaactggc acgacaccac tgtagattgt
      481 tcagaagatt cattttggct tgatgtccaa ggtgactcta tgacagcacc ggcagggtta
      541 agcattccag aaggaatgat aattctggtt gatcccgaag tcgaaccaag aaacggcaag
      601 ctggttgttg caaaattaga aggtgaaaac gaggccacat tcaaaaaatt agttatggat
      661 gcaggccgaa agtttttaaa accattaaac ccacaatatc cgatgataga aatcaacgga
      721 aactgcaaaa tcattggcgt agttgttgac gcaaaactcg caaatcttcc agctgcaaac
      781 gacgaaaact acgctttagt agcttaataa cactgatagt gctagtgtag atcactacta
      841 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      901 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      961 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1021 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
     1081 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1141 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1201 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1261 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1321 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1381 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1441 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     1501 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1561 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca
     1621 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1681 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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