LOCUS       BBa_I739016             1813 bp    DNA     linear       22-Nov-2024
DEFINITION  Double regulated polycistronic cI +LVA / ECFP +LVA expression 
            cassette
ACCESSION   BBa_I739016
VERSION     BBa_I739016.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=R0011
     misc_feature    50..68
                     /label=OR1
     misc_feature    69..87
                     /label=OR1
     misc_feature    50..87
                     /label=R0053 (2x OR1)
     promoter        1..87
                     /label=BBa_I739103
     misc_feature    100..103
                     /label=conserved
     RBS             96..107
                     /label=BBa_B0034
     CDS             117..824
                     /label=cI lambda
     misc_feature    825..857
                     /label=LVA
     misc_feature    864..888
                     /label=Help:Barcodes
     CDS             114..888
                     /label=BBa_C0051
     misc_feature    1..888
                     /label=BBa_I739008
     misc_feature    901..904
                     /label=conserved
     RBS             897..908
                     /label=BBa_B0034
     CDS             915..1676
                     /label=CFP (LVA)
     misc_feature    1633..1670
                     /label=SsrA
     misc_feature    1671..1676
                     /label=2
     misc_feature    983..983
                     /label=A
     misc_feature    915..1676
                     /label=BBa_E0022
     misc_feature    915..1676
                     /label=BBa_E0022
     stem_loop       1696..1739
                     /label=stem_loop
     misc_feature    1685..1764
                     /label=BBa_B0010
     terminator      1685..1764
                     /label=BBa_B0010
     stem_loop       1780..1799
                     /label=T7 TE
     misc_feature    1806..1806
                     /label=stop
     polyA_site      1800..1813
                     /label=polya
     misc_feature    1773..1813
                     /label=BBa_B0012
     terminator      1773..1813
                     /label=BBa_B0012
     misc_feature    897..1813
                     /label=BBa_I739009
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactaa tttaagtgtt
       61 ctttaattat ttaagtgttc tttaatttac tagagaaaga ggagaaatac tagatgagca
      121 caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt
      181 atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga
      241 tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg
      301 ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca
      361 gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg
      421 agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta
      481 ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct
      541 ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg
      601 acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag
      661 ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt
      721 ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg
      781 tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa
      841 actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagaaag
      901 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      961 tggtcgagct ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg
     1021 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1081 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
     1141 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1201 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1261 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1321 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
     1381 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
     1441 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1501 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     1561 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1621 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
     1681 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1741 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1801 ttctgcgttt ata
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