LOCUS BBa_I739020 4004 bp DNA linear 25-Nov-2024 DEFINITION Composite of BBa_I739018 and BBa_I739019 ACCESSION BBa_I739020 VERSION BBa_I739020.1 FEATURES Location/Qualifiers promoter 1..55 /label=R0062 misc_feature 56..63 /label=Scar misc_feature 64..82 /label=OR1 misc_feature 83..101 /label=OR1 misc_feature 64..101 /label=R0053 (2x OR1) promoter 1..101 /label=BBa_I739104 misc_feature 114..117 /label=conserved RBS 110..121 /label=BBa_B0034 CDS 131..838 /label=cI lambda misc_feature 839..871 /label=LVA misc_feature 878..902 /label=Help:Barcodes CDS 128..902 /label=BBa_C0051 stem_loop 922..965 /label=stem_loop misc_feature 911..990 /label=BBa_B0010 terminator 911..990 /label=BBa_B0010 stem_loop 1006..1025 /label=T7 TE misc_feature 1032..1032 /label=stop polyA_site 1026..1039 /label=polya misc_feature 999..1039 /label=BBa_B0012 terminator 999..1039 /label=BBa_B0012 terminator 911..1039 /label=BBa_B0015 misc_feature 1..1039 /label=BBa_I739006 promoter 1048..1102 /label=R0062 misc_feature 1103..1110 /label=Scar misc_feature 1111..1128 /label=OR2 misc_feature 1129..1146 /label=OR2 misc_feature 1111..1146 /label=R0051 (2x OR2) promoter 1048..1146 /label=BBa_I739105 misc_feature 1159..1162 /label=conserved RBS 1155..1166 /label=BBa_B0034 CDS 1173..1820 /label=cII p22 misc_feature 1821..1853 /label=LVA misc_feature 1854..1859 /label=stop misc_feature 1173..1859 /label=BBa_C0053 misc_feature 1860..1884 /label=Help:Barcodes CDS 1173..1859 /label=BBa_C0053 stem_loop 1904..1947 /label=stem_loop misc_feature 1893..1972 /label=BBa_B0010 terminator 1893..1972 /label=BBa_B0010 stem_loop 1988..2007 /label=T7 TE misc_feature 2014..2014 /label=stop polyA_site 2008..2021 /label=polya misc_feature 1981..2021 /label=BBa_B0012 terminator 1981..2021 /label=BBa_B0012 terminator 1893..2021 /label=BBa_B0015 misc_feature 1048..2021 /label=BBa_I739007 misc_feature 1..2021 /label=BBa_I739018 promoter 2030..2077 /label=R0040 misc_feature 2078..2095 /label=OR1 misc_feature 2096..2113 /label=OR1 misc_feature 2078..2113 /label=R0053 (2x OR1) promoter 2030..2113 /label=BBa_I739106 misc_feature 2126..2129 /label=conserved RBS 2122..2133 /label=BBa_B0034 CDS 2140..2814 /label=mrfp1 misc_feature 2854..2859 /label=Stop misc_feature 2815..2853 /label=LVA misc_feature 2140..2142 /label=Start misc_feature 2140..2859 /label=BBa_J04051 stem_loop 2879..2922 /label=stem_loop misc_feature 2868..2947 /label=BBa_B0010 terminator 2868..2947 /label=BBa_B0010 stem_loop 2963..2982 /label=T7 TE misc_feature 2989..2989 /label=stop polyA_site 2983..2996 /label=polya misc_feature 2956..2996 /label=BBa_B0012 terminator 2956..2996 /label=BBa_B0012 promoter 3005..3047 /label=R0051 misc_feature 3048..3082 /label=LacI binding site misc_feature 3048..3082 /label=R0010 promoter 3005..3082 /label=BBa_I739107 misc_feature 3095..3098 /label=conserved RBS 3091..3102 /label=BBa_B0034 misc_feature 3109..3828 /label=BBa_E0040 misc_feature 3823..3861 /label=LVA misc_feature 3862..3867 /label=Double Stop misc_feature 3109..3111 /label=Start misc_feature 3109..3867 /label=BBa_J04031 stem_loop 3887..3930 /label=stem_loop misc_feature 3876..3955 /label=BBa_B0010 terminator 3876..3955 /label=BBa_B0010 stem_loop 3971..3990 /label=T7 TE misc_feature 3997..3997 /label=stop polyA_site 3991..4004 /label=polya misc_feature 3964..4004 /label=BBa_B0012 terminator 3964..4004 /label=BBa_B0012 misc_feature 2030..4004 /label=BBa_I739019 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagatttaag tgttctttaa ttatttaagt gttctttaat ttactagaga aagaggagaa 121 atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg 181 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc 241 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt 301 aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag 361 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact 421 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga 481 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag 541 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa 601 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg 661 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga 721 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga 781 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc 841 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc 901 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 961 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1021 gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa 1081 tggtttgtta tagtcgaata aatactagag taacaccgtg cgtgttgata acaccgtgcg 1141 tgttgatact agagaaagag gagaaatact agatgaatac acaattgatg ggtgagcgta 1201 ttcgcgctcg aagaaaaaaa ctcaagatta gacaagccgc tcttggtaag atggtgggag 1261 tgtctaatgt tgcaatatcg caatgggagc gctcggagac tgagccaaat ggggagaacc 1321 tgttggcact ttcgaaggct cttcagtgct cccctgacta tttgctgaaa ggagatttaa 1381 gccagacaaa cgttgcctat catagtaggc atgagccaag aggatcatac cctcttatca 1441 gttgggtaag cgcagggcaa tggatggaag ctgtagaacc ttatcacaag cgcgcgatag 1501 agaactggca cgacaccact gtagattgtt cagaagattc attttggctt gatgtccaag 1561 gtgactctat gacagcaccg gcagggttaa gcattccaga aggaatgata attctggttg 1621 atcccgaagt cgaaccaaga aacggcaagc tggttgttgc aaaattagaa ggtgaaaacg 1681 aggccacatt caaaaaatta gttatggatg caggccgaaa gtttttaaaa ccattaaacc 1741 cacaatatcc gatgatagaa atcaacggaa actgcaaaat cattggcgta gttgttgacg 1801 caaaactcgc aaatcttcca gctgcaaacg acgaaaacta cgctttagta gcttaataac 1861 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1921 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1981 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt 2041 gatagagatt gacatcccta tcagtgatag agatactatt taagtgttct ttaatattta 2101 agtgttcttt aattactaga gaaagaggag aaatactaga tggcttcctc cgaagacgtt 2161 atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc 2221 gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa 2281 gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac 2341 ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc 2401 ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt 2461 acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc 2521 aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc 2581 gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg 2641 aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt 2701 cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac 2761 tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgctaggcct 2821 gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat 2881 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2941 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3001 agagtaacac cgtgcgtgtt gactatttta cctctggcgg tgataattgt gtggaattgt 3061 gagcggataa caatttcaca catactagag aaagaggaga aatactagat gcgtaaagga 3121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 3181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 3241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 3301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc 3361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 3421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag 3481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac 3541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac 3601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa 3661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 3721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta 3781 acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac 3841 gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa aacgaaaggc 3901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 3961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //