LOCUS       BBa_I739021             1746 bp    DNA     linear       22-Dec-2024
DEFINITION  Single regulated polycistronic P22 cII +LVA / EYFP +LVA expression 
            cassette
ACCESSION   BBa_I739021
VERSION     BBa_I739021.1  
FEATURES             Location/Qualifiers
     misc_feature    36..43
                     /label=Scar
     promoter        1..35
                     /label=J23100
     misc_feature    64..83
                     /label=TetR 1
     misc_feature    44..63
                     /label=TetR 1
     misc_feature    44..83
                     /label=R0040 (2x TetR 1)
     promoter        1..83
                     /label=BBa_I739101
     misc_feature    96..99
                     /label=conserved
     RBS             92..103
                     /label=BBa_B0034
     CDS             110..757
                     /label=cII p22
     misc_feature    758..790
                     /label=LVA
     misc_feature    791..796
                     /label=stop
     misc_feature    110..796
                     /label=BBa_C0053
     misc_feature    797..821
                     /label=Help:Barcodes
     CDS             110..796
                     /label=BBa_C0053
     misc_feature    1..821
                     /label=BBa_I739014
     misc_feature    834..837
                     /label=conserved
     RBS             830..841
                     /label=BBa_B0034
     CDS             848..1609
                     /label=YFP (LVA)
     misc_feature    1604..1609
                     /label=2
     misc_feature    1565..1603
                     /label=SsrA
     misc_feature    1089..1089
                     /label=A (Q->L)
     misc_feature    848..1609
                     /label=BBa_E0032
     misc_feature    848..1609
                     /label=BBa_E0032
     stem_loop       1629..1672
                     /label=stem_loop
     misc_feature    1618..1697
                     /label=BBa_B0010
     terminator      1618..1697
                     /label=BBa_B0010
     stem_loop       1713..1732
                     /label=T7 TE
     misc_feature    1739..1739
                     /label=stop
     polyA_site      1733..1746
                     /label=polya
     misc_feature    1706..1746
                     /label=BBa_B0012
     terminator      1706..1746
                     /label=BBa_B0012
     misc_feature    830..1746
                     /label=BBa_I739005
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagtccctat cagtgataga
       61 gattccctat cagtgataga gattactaga gaaagaggag aaatactaga tgaatacaca
      121 attgatgggt gagcgtattc gcgctcgaag aaaaaaactc aagattagac aagccgctct
      181 tggtaagatg gtgggagtgt ctaatgttgc aatatcgcaa tgggagcgct cggagactga
      241 gccaaatggg gagaacctgt tggcactttc gaaggctctt cagtgctccc ctgactattt
      301 gctgaaagga gatttaagcc agacaaacgt tgcctatcat agtaggcatg agccaagagg
      361 atcataccct cttatcagtt gggtaagcgc agggcaatgg atggaagctg tagaacctta
      421 tcacaagcgc gcgatagaga actggcacga caccactgta gattgttcag aagattcatt
      481 ttggcttgat gtccaaggtg actctatgac agcaccggca gggttaagca ttccagaagg
      541 aatgataatt ctggttgatc ccgaagtcga accaagaaac ggcaagctgg ttgttgcaaa
      601 attagaaggt gaaaacgagg ccacattcaa aaaattagtt atggatgcag gccgaaagtt
      661 tttaaaacca ttaaacccac aatatccgat gatagaaatc aacggaaact gcaaaatcat
      721 tggcgtagtt gttgacgcaa aactcgcaaa tcttccagct gcaaacgacg aaaactacgc
      781 tttagtagct taataacact gatagtgcta gtgtagatca ctactagaga aagaggagaa
      841 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      901 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      961 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1021 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca
     1081 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1141 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1201 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1261 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1321 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1381 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1441 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1501 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1561 caagaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat actagagcca
     1621 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1681 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1741 tttata
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