BBa_I739021
type: ComponentDefinition
persistent identity: BBa I739021
display id: BBa_I739021
version: 1
was generated by: iGEM to SBOL conversion
description: Single regulated polycistronic P22 cII +LVA / EYFP +LVA expression cassette
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: as
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: asdf
star: false
creator: Stefan Luzi
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I739005
component: BBa_I739014
sequence annotation: BBa_I739014
sequence annotation: BBa_I739005
sequence: https://synbiohub.org/public/igem/BBa_I739021_sequence/1

BBa_I739005
type: Component
persistent identity: component2266464
display id: component2266464
version: 1
top level: BBa_I739021
definition: BBa_I739005
access: public

BBa_I739014
type: Component
persistent identity: component2266450
display id: component2266450
version: 1
top level: BBa_I739021
definition: BBa_I739014
access: public

BBa_I739014
type: SequenceAnnotation
persistent identity: annotation2266450
display id: annotation2266450
version: 1
top level: BBa_I739021
location: https://synbiohub.org/public/igem/BBa_I739021/annotation2266450/range2266450/1
component: BBa_I739014

https://synbiohub.org/public/igem/BBa_I739021/annotation2266450/range2266450/1
type: Range
persistent identity: range2266450
display id: range2266450
version: 1
top level: BBa_I739021
direction: direction/reverse
start: 1
end: 821
orientation: inline

BBa_I739005
type: SequenceAnnotation
persistent identity: annotation2266464
display id: annotation2266464
version: 1
top level: BBa_I739021
location: https://synbiohub.org/public/igem/BBa_I739021/annotation2266464/range2266464/1
component: BBa_I739005

https://synbiohub.org/public/igem/BBa_I739021/annotation2266464/range2266464/1
type: Range
persistent identity: range2266464
display id: range2266464
version: 1
top level: BBa_I739021
direction: direction/reverse
start: 830
end: 1746
orientation: inline

BBa_I739005
type: ComponentDefinition
persistent identity: BBa I739005
display id: BBa_I739005
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless expression cassette for EYFP +LVA (B0034.E0032.B0015)
created: 2007-09-26T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: synthetic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The Biobrick contains the Ribosome binding site BBa_B0034 upstream of a LVA tagged YFP gene (short halflife). The transcription is terminated by the double terminator BBa_B0015. This construct can be used as second cistron expressing a reporter gene in a multicistronic construct.
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2208
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: second intron for the expression of YFP-LVA
star: false
creator: Christian Kemmer, Stefan Luzi
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0032
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I739005_sequence/1

BBa_E0032
type: Component
persistent identity: component1945216
display id: component1945216
version: 1
top level: BBa_I739005
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component1945214
display id: component1945214
version: 1
top level: BBa_I739005
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1945222
display id: component1945222
version: 1
top level: BBa_I739005
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1945220
display id: component1945220
version: 1
top level: BBa_I739005
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1945214
display id: annotation1945214
version: 1
top level: BBa_I739005
location: https://synbiohub.org/public/igem/BBa_I739005/annotation1945214/range1945214/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I739005/annotation1945214/range1945214/1
type: Range
persistent identity: range1945214
display id: range1945214
version: 1
top level: BBa_I739005
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1945216
display id: annotation1945216
version: 1
top level: BBa_I739005
location: https://synbiohub.org/public/igem/BBa_I739005/annotation1945216/range1945216/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I739005/annotation1945216/range1945216/1
type: Range
persistent identity: range1945216
display id: range1945216
version: 1
top level: BBa_I739005
direction: direction/forward
start: 19
end: 780
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1945220
display id: annotation1945220
version: 1
top level: BBa_I739005
location: https://synbiohub.org/public/igem/BBa_I739005/annotation1945220/range1945220/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I739005/annotation1945220/range1945220/1
type: Range
persistent identity: range1945220
display id: range1945220
version: 1
top level: BBa_I739005
direction: direction/forward
start: 789
end: 868
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1945222
display id: annotation1945222
version: 1
top level: BBa_I739005
location: https://synbiohub.org/public/igem/BBa_I739005/annotation1945222/range1945222/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I739005/annotation1945222/range1945222/1
type: Range
persistent identity: range1945222
display id: range1945222
version: 1
top level: BBa_I739005
direction: direction/forward
start: 877
end: 917
orientation: inline

BBa_I739014
type: ComponentDefinition
persistent identity: BBa I739014
display id: BBa_I739014
version: 1
was generated by: iGEM to SBOL conversion
description: Single regulated intermediate for P22 cII +LVA (I739101.B0034.C0053)
created: 2007-10-13T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: dsf
owned by: james
owned by: myers
mutable description: sdf
discontinued: false
dominant: false
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: df
star: false
creator: Stefan Luzi
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_C0053
component: BBa_B0034
component: BBa_I739101
sequence annotation: BBa_I739101
sequence annotation: BBa_B0034
sequence annotation: BBa_C0053
sequence: https://synbiohub.org/public/igem/BBa_I739014_sequence/1

BBa_C0053
type: Component
persistent identity: component2245691
display id: component2245691
version: 1
top level: BBa_I739014
definition: c2 P22
access: public

BBa_B0034
type: Component
persistent identity: component2245689
display id: component2245689
version: 1
top level: BBa_I739014
definition: BBa_B0034
access: public

BBa_I739101
type: Component
persistent identity: component2245687
display id: component2245687
version: 1
top level: BBa_I739014
definition: BBa_I739101
access: public

BBa_I739101
type: SequenceAnnotation
persistent identity: annotation2245687
display id: annotation2245687
version: 1
top level: BBa_I739014
location: https://synbiohub.org/public/igem/BBa_I739014/annotation2245687/range2245687/1
component: BBa_I739101

https://synbiohub.org/public/igem/BBa_I739014/annotation2245687/range2245687/1
type: Range
persistent identity: range2245687
display id: range2245687
version: 1
top level: BBa_I739014
direction: direction/reverse
start: 1
end: 83
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2245689
display id: annotation2245689
version: 1
top level: BBa_I739014
location: https://synbiohub.org/public/igem/BBa_I739014/annotation2245689/range2245689/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I739014/annotation2245689/range2245689/1
type: Range
persistent identity: range2245689
display id: range2245689
version: 1
top level: BBa_I739014
direction: direction/reverse
start: 92
end: 103
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation2245691
display id: annotation2245691
version: 1
top level: BBa_I739014
location: https://synbiohub.org/public/igem/BBa_I739014/annotation2245691/range2245691/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_I739014/annotation2245691/range2245691/1
type: Range
persistent identity: range2245691
display id: range2245691
version: 1
top level: BBa_I739014
direction: direction/forward
start: 110
end: 796
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: iGEM to SBOL conversion
description: c2 repressor from Salmonella phage P22 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cII p22
sequence annotation: BBa_C0053
sequence annotation: LVA
sequence annotation: stop
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 648
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
top level: c2 P22
direction: direction/forward
start: 649
end: 681
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
top level: c2 P22
direction: direction/forward
start: 682
end: 687
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
top level: c2 P22
direction: direction/forward
start: 688
end: 712
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

BBa_I739101
type: ComponentDefinition
persistent identity: BBa I739101
display id: BBa_I739101
version: 1
was generated by: iGEM to SBOL conversion
description: Double Promoter (constitutive / TetR, negative)
created: 2007-10-11T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: sdfasdf
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: asdf
star: false
creator: Stefan Luzi
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: Scar
sequence annotation: TetR 1
sequence annotation: R0040 (2x TetR 1)
sequence: https://synbiohub.org/public/igem/BBa_I739101_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation1946100
display id: annotation1946100
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
type: Range
persistent identity: range1946100
display id: range1946100
version: 1
top level: BBa_I739101
direction: direction/forward
start: 1
end: 35
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946099
display id: annotation1946099
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
type: Range
persistent identity: range1946099
display id: range1946099
version: 1
top level: BBa_I739101
direction: direction/forward
start: 36
end: 43
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946102
display id: annotation1946102
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
type: Range
persistent identity: range1946102
display id: range1946102
version: 1
top level: BBa_I739101
direction: direction/forward
start: 44
end: 63
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946257
display id: annotation1946257
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
type: Range
persistent identity: range1946257
display id: range1946257
version: 1
top level: BBa_I739101
direction: direction/forward
start: 44
end: 83
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946101
display id: annotation1946101
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
type: Range
persistent identity: range1946101
display id: range1946101
version: 1
top level: BBa_I739101
direction: direction/forward
start: 64
end: 83
orientation: inline

https://synbiohub.org/public/igem/BBa_I739021_sequence/1
type: Sequence
persistent identity: BBa I739021 sequence
display id: BBa_I739021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagattactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I739005_sequence/1
type: Sequence
persistent identity: BBa I739005 sequence
display id: BBa_I739005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I739014_sequence/1
type: Sequence
persistent identity: BBa I739014 sequence
display id: BBa_I739014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagattactagagaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0053_sequence/1
type: Sequence
persistent identity: BBa C0053 sequence
display id: BBa_C0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I739101_sequence/1
type: Sequence
persistent identity: BBa I739101 sequence
display id: BBa_I739101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z