LOCUS BBa_I741049 1996 bp DNA linear 22-Dec-2024 ACCESSION BBa_I741049 VERSION BBa_I741049.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 RBS 1053..1063 /label=RBS-4\Weaker RBS 1059..1062 /label=RBS misc_feature 1053..1063 /label=BBa_B0033 RBS 1053..1063 /label=BBa_B0033 CDS 1070..1996 /label=BBa_J09271 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gactactaga tgaagaatca 1081 agcgcatccg attattgtcg tcaaacgacg caaagccaaa agccacgggg cagcacatgg 1141 atcgtggaag attgcttatg ccgactttat gactgcgatg atggcctttt ttctggtgat 1201 gtggctgatc tccatctcca gcccaaaaga gctgattcag attgcggagt acttccggac 1261 tccactggcg actgcggtta cgggcggcga tcgcatttct aatagtgaaa gcccaattcc 1321 cggcggtggt gatgattaca cccaaagcca gggggaagtg aataagcagc cgaacatcga 1381 agagctgaaa aaacgcatgg agcaaagtcg attgcggaaa ttgcggggtg atctcgacca 1441 gttgatagag tccgatccga aactgcgggc gttacgtccc catctcaaaa tcgatctggt 1501 ccaggaaggt ctacgtattc agatcatcga tagccagaat cgcccgatgt ttagaaccgg 1561 cagtgccgat gtcgaaccct atatgcgcga cattctgcgc gccattgcgc ctgtactgaa 1621 cggtattccc aaccgtatta gcctttcagg tcataccgat gatttcccct acgccagcgg 1681 tgagaaagga tatagcaact gggagctttc tgccgatcgg gccaatgcat cccgccgcga 1741 actgatggtc ggagggttgg atagcggcaa agtgttacgt gtcgtcggca tggcggcaac 1801 gatgcgctta agcgatcgcg gacctgatga tgccgtcaac cgtcgcatca gcctgctggt 1861 actgaacaaa caagccgaac aggccatttt gcatgaaaac gccgaaagcc agaatgagcc 1921 agtaagcgcc ctggaaaaac ctgaggttgc accacaggtc agtgttccca caatgccatc 1981 agccgaaccg aggtga // LOCUS BBa_I741001 1996 bp DNA linear 22-Dec-2024 DEFINITION HSL Receiver Construct ACCESSION BBa_I741001 VERSION BBa_I741001.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 RBS 1053..1063 /label=RBS-4\Weaker RBS 1059..1062 /label=RBS misc_feature 1053..1063 /label=BBa_B0033 RBS 1053..1063 /label=BBa_B0033 CDS 1070..1996 /label=BBa_J09271 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gactactaga tgaagaatca 1081 agcgcatccg attattgtcg tcaaacgacg caaagccaaa agccacgggg cagcacatgg 1141 atcgtggaag attgcttatg ccgactttat gactgcgatg atggcctttt ttctggtgat 1201 gtggctgatc tccatctcca gcccaaaaga gctgattcag attgcggagt acttccggac 1261 tccactggcg actgcggtta cgggcggcga tcgcatttct aatagtgaaa gcccaattcc 1321 cggcggtggt gatgattaca cccaaagcca gggggaagtg aataagcagc cgaacatcga 1381 agagctgaaa aaacgcatgg agcaaagtcg attgcggaaa ttgcggggtg atctcgacca 1441 gttgatagag tccgatccga aactgcgggc gttacgtccc catctcaaaa tcgatctggt 1501 ccaggaaggt ctacgtattc agatcatcga tagccagaat cgcccgatgt ttagaaccgg 1561 cagtgccgat gtcgaaccct atatgcgcga cattctgcgc gccattgcgc ctgtactgaa 1621 cggtattccc aaccgtatta gcctttcagg tcataccgat gatttcccct acgccagcgg 1681 tgagaaagga tatagcaact gggagctttc tgccgatcgg gccaatgcat cccgccgcga 1741 actgatggtc ggagggttgg atagcggcaa agtgttacgt gtcgtcggca tggcggcaac 1801 gatgcgctta agcgatcgcg gacctgatga tgccgtcaac cgtcgcatca gcctgctggt 1861 actgaacaaa caagccgaac aggccatttt gcatgaaaac gccgaaagcc agaatgagcc 1921 agtaagcgcc ctggaaaaac ctgaggttgc accacaggtc agtgttccca caatgccatc 1981 agccgaaccg aggtga //