LOCUS       BBa_I741100             1187 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter (I741015) and GFP (E0240)
ACCESSION   BBa_I741100
VERSION     BBa_I741100.1  
FEATURES             Location/Qualifiers
     misc_feature    13..13
                     /label=Start Transcription (left) xylAP
     promoter        19..24
                     /label=xylAP -10 Region
     promoter        43..48
                     /label=xylA P -35 Region
     misc_feature    48..63
                     /label=XylR binding halfsite
     misc_feature    69..84
                     /label=XylR binding halfsite
     misc_feature    84..102
                     /label=CRP-cAMP dual transcriptional regulator
     misc_feature    199..214
                     /label=XylR binding halfsite
     misc_feature    219..234
                     /label=XylR binding halfsite
     promoter        241..246
                     /label=xylFP -35 Region
     promoter        263..268
                     /label=xylFP -10 Region
     misc_feature    275..275
                     /label=Start Transcription (right) xylFp
     promoter        1..301
                     /label=BBa_I741015
     RBS             310..324
                     /label=RBS-1\Strong
     RBS             317..321
                     /label=RBS
     misc_feature    310..324
                     /label=BBa_B0030
     RBS             310..324
                     /label=BBa_B0030
     CDS             331..1050
                     /label=GFP protein
     CDS             331..1050
                     /label=BBa_E0040
     stem_loop       1070..1113
                     /label=stem_loop
     misc_feature    1059..1138
                     /label=BBa_B0010
     terminator      1059..1138
                     /label=BBa_B0010
     stem_loop       1154..1173
                     /label=T7 TE
     misc_feature    1180..1180
                     /label=stop
     polyA_site      1174..1187
                     /label=polya
     misc_feature    1147..1187
                     /label=BBa_B0012
     terminator      1147..1187
                     /label=BBa_B0012
ORIGIN
        1 ggtgatggat gatgtcgtaa tattgggcac tccctttcag ttgctcaatt atgttatttc
       61 acactgctat tgagataatt cacaagtgtg cgctcgctcg caaaataaaa tggaatgatg
      121 aaactgggta attcctcgaa gagaaaaatg caataagtac aattgcgcaa caaaagtaag
      181 atctcggtca taaatcaaga aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa
      241 ttgttttccc ctgtttagtt gctaaaaatt ggttacgttt atcgcggtga ttgttactta
      301 ttactagaga ttaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga
      361 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      421 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      481 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      541 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      601 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      661 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      721 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      781 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      841 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      901 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      961 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     1021 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
     1081 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1141 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//