BBa_J15001 1 BBa_J15001 strong synthetic E. coli ribosome binding site with SacI site. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Synthetic. This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector. false false _163_ 0 837 163 Not in stock false Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest. false Chris French annotation1938046 1 rbs range1938046 1 4 10 annotation1938045 1 SacI range1938045 1 1 3 BBa_I742114 1 BBa_I742114 Feruloyl-CoA synthase (fcs) 2007-10-18T11:00:00Z 2015-08-31T04:08:02Z Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002) gene is from Pseudomonas fluorescens encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) produces feruloyl CoA from ferrulic acid catalyses the penultimate of the vanillin biosynthesis pathway false false _123_ 0 1964 9 Not in stock false Gene was synthesised by geneart, as P. fluorescens has a high GC genome. Coding region was optimised for gene expression in E. coli false sarah hollingshead annotation1949792 1 fcs coding region range1949792 1 1 1773 BBa_I742115 1 BBa_I742115 fcs gene with ribosome binding site 2007-10-18T11:00:00Z 2015-08-31T04:08:02Z Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002) gene was isolated from Pseudomonas fluorescens encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) produces feruloyl CoA from ferrulic acid catalyses the penultimate of the vanillin biosynthesis pathway false true _123_ 0 1964 9 It's complicated false Synthesised by Geneart with codon optimisation for expression in Escherichia coli false sarah hollingshead component1949802 1 BBa_I742114 component1949800 1 BBa_J15001 annotation1949800 1 BBa_J15001 range1949800 1 1 10 annotation1949802 1 BBa_I742114 range1949802 1 17 1789 BBa_I742115_sequence 1 ctcaaggaggtactagatgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataa BBa_J15001_sequence 1 ctcaaggagg BBa_I742114_sequence 1 atgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z