BBa_I742124 1 Plac(rev) Reverse complement Lac promoter 2007-10-21T11:00:00Z 2015-08-31T04:08:02Z This sequence is part of the E.coli K12 genome. This is an 'upside-down' version of I14032. It is a strong, constitual promoter. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional reglatory possibility that this gives. The upstream sequence is non-functional and simply there to faciliate gel analysis or to physically enable the part to be recomineered (see the Edinburgh 2007 project where it is flanked by dif-sites). false false _123_ 0 1968 9 It's complicated true When dealing with reverse complements of a locus, suffix and prefix need be fitted in opposite order. false Caroline Dahl annotation1955298 1 Plac -35 range1955298 1 61 66 annotation1955267 1 LacI binding site range1955267 1 11 31 annotation1955266 1 CAP binding site range1955266 1 78 115 annotation1955299 1 Plac -10 range1955299 1 38 43 BBa_I742127 1 BBa_I742127 dif-site + reverse Plac 2007-10-21T11:00:00Z 2015-08-31T04:08:03Z Both dif and Plac are naturally occurring genetic elements in E. coli K12. The dif-site is a site for recombination at bacterial replication. When combined with a downstream direct repeat dif-site, flanked sequence will excise (including the reverse Plac). When combined with a reverse dif-site downstream, flanked DNA will invert. Please note that the above hypothetical workings needs be tested for frequency and occurrance in vivo. false false _123_ 0 1968 9 Not in stock false See individual bricks for details. false Caroline Dahl component1952169 1 BBa_I742101 component1952170 1 BBa_I742124 annotation1952169 1 BBa_I742101 range1952169 1 1 31 annotation1952170 1 BBa_I742124 range1952170 1 40 242 BBa_I742101 1 difF dif site with forward orientation 2007-08-07T11:00:00Z 2015-08-31T04:08:02Z E. coli K12 Released HQ 2013 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct repeats of the dif site are vital to DNA monomerisation during bacterial septation. As with other recombinase sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enables recombination that is temporally isolated to septation. false false _123_ 0 1965 9 In stock true Constructed as two complimentary oligonucleotides. true Xiaonan Wang annotation1940992 1 SacI range1940992 1 1 3 annotation1955264 1 dif site (forward) range1955264 1 4 31 BBa_I742127_sequence 1 ctcggtgcgcataatgtatattatgttaaattactagagctctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg BBa_I742124_sequence 1 ctctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg BBa_I742101_sequence 1 ctcggtgcgcataatgtatattatgttaaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z