BBa_I742132
type: ComponentDefinition
persistent identity: BBa I742132
display id: BBa_I742132
version: 1
was generated by: iGEM to SBOL conversion
description: Division-PoPper: Cell division --> PoPS
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Please refer to individual parts. They are all more or less modified versions of E. coli K12 loci and its phage lambda.
owned by: james
owned by: myers
mutable description: This device gives PoPS-output on cell division. The DNA flanked by the opposing repeats of the dif-site is inverted and allows transcription of both any downstream element and a repressor which, upon sufficient build-up, switches off the promoter which controls downstream transcription. Inversion only occurs when the XerCD complex is activated, and this only occurs at cell division. Therefore this device produces a short burst of PoPS as a function of cell division. Any added downstream elements will likewise be activated, briefly, as a function of cell division.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Again, please refer to to part in question. However, as a general rule, all parts in 'non-default', bottom DNA strand need be synthesised in reverse complementary fashion. You will encounter parts that are called 'upside-down' or 'reverse complementary' for this reason.
star: false
creator: Caroline Dahl
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_I742102
component: BBa_R0040
component: BBa_I742130
component: BBa_I742131
component: BBa_I742129
component: BBa_C0040
component: BBa_B0034
component: BBa_I742126
component: BBa_I742101
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_I742101
sequence annotation: BBa_I742126
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_I742129
sequence annotation: BBa_I742131
sequence annotation: BBa_I742130
sequence annotation: BBa_R0040
sequence annotation: BBa_I742102
sequence: https://synbiohub.org/public/igem/BBa_I742132_sequence/1

BBa_I742102
type: Component
persistent identity: component2280668
display id: component2280668
version: 1
top level: BBa_I742132
definition: difR
access: public

BBa_R0040
type: Component
persistent identity: component2280661
display id: component2280661
version: 1
top level: BBa_I742132
definition: p(tetR)
access: public

BBa_I742130
type: Component
persistent identity: component2280660
display id: component2280660
version: 1
top level: BBa_I742132
definition: revRBS
access: public

BBa_I742131
type: Component
persistent identity: component2280659
display id: component2280659
version: 1
top level: BBa_I742132
definition: rev.cI
access: public

BBa_I742129
type: Component
persistent identity: component2280657
display id: component2280657
version: 1
top level: BBa_I742132
definition: BBa_I742129
access: public

BBa_C0040
type: Component
persistent identity: component2280656
display id: component2280656
version: 1
top level: BBa_I742132
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2280652
display id: component2280652
version: 1
top level: BBa_I742132
definition: BBa_B0034
access: public

BBa_I742126
type: Component
persistent identity: component2280650
display id: component2280650
version: 1
top level: BBa_I742132
definition: revPlam
access: public

BBa_I742101
type: Component
persistent identity: component2280649
display id: component2280649
version: 1
top level: BBa_I742132
definition: difF
access: public

BBa_I14032
type: Component
persistent identity: component2280646
display id: component2280646
version: 1
top level: BBa_I742132
definition: P(Lac) IQ
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2280646
display id: annotation2280646
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280646/range2280646/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_I742132/annotation2280646/range2280646/1
type: Range
persistent identity: range2280646
display id: range2280646
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_I742101
type: SequenceAnnotation
persistent identity: annotation2280649
display id: annotation2280649
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280649/range2280649/1
component: BBa_I742101

https://synbiohub.org/public/igem/BBa_I742132/annotation2280649/range2280649/1
type: Range
persistent identity: range2280649
display id: range2280649
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 46
end: 76
orientation: inline

BBa_I742126
type: SequenceAnnotation
persistent identity: annotation2280650
display id: annotation2280650
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280650/range2280650/1
component: BBa_I742126

https://synbiohub.org/public/igem/BBa_I742132/annotation2280650/range2280650/1
type: Range
persistent identity: range2280650
display id: range2280650
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 85
end: 133
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2280652
display id: annotation2280652
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280652/range2280652/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I742132/annotation2280652/range2280652/1
type: Range
persistent identity: range2280652
display id: range2280652
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 142
end: 153
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2280656
display id: annotation2280656
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280656/range2280656/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I742132/annotation2280656/range2280656/1
type: Range
persistent identity: range2280656
display id: range2280656
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 160
end: 844
orientation: inline

BBa_I742129
type: SequenceAnnotation
persistent identity: annotation2280657
display id: annotation2280657
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280657/range2280657/1
component: BBa_I742129

https://synbiohub.org/public/igem/BBa_I742132/annotation2280657/range2280657/1
type: Range
persistent identity: range2280657
display id: range2280657
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 853
end: 898
orientation: inline

BBa_I742131
type: SequenceAnnotation
persistent identity: annotation2280659
display id: annotation2280659
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280659/range2280659/1
component: BBa_I742131

https://synbiohub.org/public/igem/BBa_I742132/annotation2280659/range2280659/1
type: Range
persistent identity: range2280659
display id: range2280659
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 907
end: 1656
orientation: inline

BBa_I742130
type: SequenceAnnotation
persistent identity: annotation2280660
display id: annotation2280660
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280660/range2280660/1
component: BBa_I742130

https://synbiohub.org/public/igem/BBa_I742132/annotation2280660/range2280660/1
type: Range
persistent identity: range2280660
display id: range2280660
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 1665
end: 1674
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2280661
display id: annotation2280661
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280661/range2280661/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I742132/annotation2280661/range2280661/1
type: Range
persistent identity: range2280661
display id: range2280661
version: 1
top level: BBa_I742132
direction: direction/forward
start: 1683
end: 1736
orientation: inline

BBa_I742102
type: SequenceAnnotation
persistent identity: annotation2280668
display id: annotation2280668
version: 1
top level: BBa_I742132
location: https://synbiohub.org/public/igem/BBa_I742132/annotation2280668/range2280668/1
component: BBa_I742102

https://synbiohub.org/public/igem/BBa_I742132/annotation2280668/range2280668/1
type: Range
persistent identity: range2280668
display id: range2280668
version: 1
top level: BBa_I742132
direction: direction/reverse
start: 1745
end: 1775
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

revPlam
type: ComponentDefinition
persistent identity: BBa I742126
display id: BBa_I742126
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse lambda cI-regulated promoter
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: See BBa_R0051.
owned by: james
owned by: myers
mutable description: This is an exact 'upside down' version of BBa_R0051. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. By adding this part, we wish to emphasise the possibility of engineering both DNA strands, and the additional regulatory potential that it gives.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Any 'upside down' (reverse complementary) sequences made by PCR amplification need be made with suffix and prefix is opposite order with respect to the original default sequence.
star: false
creator: Caroline Dahl
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I742126_sequence/1

difF
type: ComponentDefinition
persistent identity: BBa I742101
display id: BBa_I742101
version: 1
was generated by: iGEM to SBOL conversion
description: dif site with forward orientation
created: 2007-08-07T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct repeats of the dif site are vital to DNA monomerisation during bacterial septation. As with other recombinase sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enables recombination that is temporally isolated to septation.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Constructed as two complimentary oligonucleotides.
star: true
creator: Xiaonan Wang
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: SacI
sequence annotation: dif site (forward)
sequence: https://synbiohub.org/public/igem/BBa_I742101_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1940992
display id: annotation1940992
version: 1
top level: difF
location: https://synbiohub.org/public/igem/BBa_I742101/annotation1940992/range1940992/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I742101/annotation1940992/range1940992/1
type: Range
persistent identity: range1940992
display id: range1940992
version: 1
top level: difF
direction: direction/forward
start: 1
end: 3
orientation: inline

dif site (forward)
type: SequenceAnnotation
persistent identity: annotation1955264
display id: annotation1955264
version: 1
top level: difF
location: https://synbiohub.org/public/igem/BBa_I742101/annotation1955264/range1955264/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I742101/annotation1955264/range1955264/1
type: Range
persistent identity: range1955264
display id: range1955264
version: 1
top level: difF
direction: direction/forward
start: 4
end: 31
orientation: inline

BBa_I742129
type: ComponentDefinition
persistent identity: BBa I742129
display id: BBa_I742129
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse terminator BBa_B0011 LuxICDABEG (+/-)
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Please see BBa_B0011 for details.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LuxICDABEG (+/-) forms a stem-loop which cancels transcription on both strands, whichever you choose to place it in. Please see BBa_B0011 for details about the terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None.
star: false
creator: Caroline Dahl
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_I742129_sequence/1

revRBS
type: ComponentDefinition
persistent identity: BBa I742130
display id: BBa_I742130
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Please refer to the original (BB_B0034).
owned by: james
owned by: myers
mutable description: DNA is double-stranded. To translate genes of both strands, you need RBSs in both strands.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Please refer to the original (BB_B0034).
star: false
creator: Caroline Dahl
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_I742130_sequence/1

rev.cI
type: ComponentDefinition
persistent identity: BBa I742131
display id: BBa_I742131
version: 1
was generated by: iGEM to SBOL conversion
description: Inverse cI repressor coding sequence.
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Please refer to the original, BBa_C0051.
owned by: james
owned by: myers
mutable description: This sequence encodes the cl repressor, only it encodes it in the 'non-default', bottom strand, rather than the straight-forward top one. When combined with the cl-responsive promoter, it enables regulatory control of both strands.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1968
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: As above.
star: false
creator: Caroline Dahl
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence annotation: cI repressor coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I742131_sequence/1

cI repressor coding sequence
type: SequenceAnnotation
persistent identity: annotation1956553
display id: annotation1956553
version: 1
top level: rev.cI
location: https://synbiohub.org/public/igem/BBa_I742131/annotation1956553/range1956553/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742131/annotation1956553/range1956553/1
type: Range
persistent identity: range1956553
display id: range1956553
version: 1
top level: rev.cI
direction: direction/reverse
start: 4
end: 750
orientation: inline

difR
type: ComponentDefinition
persistent identity: BBa I742102
display id: BBa_I742102
version: 1
was generated by: iGEM to SBOL conversion
description: dif site with reverse orientation
created: 2007-08-07T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: 'dif' (division induced filamentation) is the recombination site for XerC and XerD recombinases. Once replicated, aligned direct dif repeats are vital to DNA monomerisation during bacterial septation. As with other recombination sites, inverted repeats cause inversion of the DNA in between. However, XerCD requires activation by FtsK and thus two dif-sites (see also part BBa_I742102) enable recombination that is temporally isolated to division.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1965
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Synthesised using two oligonucleotides.
star: true
creator: Xiaonan Wang
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: SacI
sequence annotation: dif site (reverse)
sequence: https://synbiohub.org/public/igem/BBa_I742102_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1940993
display id: annotation1940993
version: 1
top level: difR
location: https://synbiohub.org/public/igem/BBa_I742102/annotation1940993/range1940993/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I742102/annotation1940993/range1940993/1
type: Range
persistent identity: range1940993
display id: range1940993
version: 1
top level: difR
direction: direction/forward
start: 1
end: 3
orientation: inline

dif site (reverse)
type: SequenceAnnotation
persistent identity: annotation1955265
display id: annotation1955265
version: 1
top level: difR
location: https://synbiohub.org/public/igem/BBa_I742102/annotation1955265/range1955265/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I742102/annotation1955265/range1955265/1
type: Range
persistent identity: range1955265
display id: range1955265
version: 1
top level: difR
direction: direction/reverse
start: 4
end: 31
orientation: inline

https://synbiohub.org/public/igem/BBa_I742132_sequence/1
type: Sequence
persistent identity: BBa I742132 sequence
display id: BBa_I742132_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagctcggtgcgcataatgtatattatgttaaattactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttatactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaataataaaaaagccggattaataatctggctttttatattctcttactagagttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcattactagagtctcctcttttactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagctcatttaacataatatacattatgcgcacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742126_sequence/1
type: Sequence
persistent identity: BBa I742126 sequence
display id: BBa_I742126_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgtta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742101_sequence/1
type: Sequence
persistent identity: BBa I742101 sequence
display id: BBa_I742101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcggtgcgcataatgtatattatgttaaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742129_sequence/1
type: Sequence
persistent identity: BBa I742129 sequence
display id: BBa_I742129_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaataataaaaaagccggattaataatctggctttttatattctct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742130_sequence/1
type: Sequence
persistent identity: BBa I742130 sequence
display id: BBa_I742130_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctcctcttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742131_sequence/1
type: Sequence
persistent identity: BBa I742131 sequence
display id: BBa_I742131_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaagctactaaagcgtagttttcgtcgtttgcagcgccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagctcaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742102_sequence/1
type: Sequence
persistent identity: BBa I742102 sequence
display id: BBa_I742102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatttaacataatatacattatgcgcacc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z