LOCUS       BBa_I742133             2430 bp    DNA     linear       22-Dec-2024
DEFINITION  CrtIB genes with PstI site mutated out
ACCESSION   BBa_I742133
VERSION     BBa_I742133.1  
FEATURES             Location/Qualifiers
     RBS             1..22
                     /label=native rbs
     CDS             23..1481
                     /label=CrtI coding region
     CDS             1498..2430
                     /label=CrtB coding region
     RBS             1482..1497
                     /label=native rbs
     misc_feature    23..25
                     /label=CrtI start
     misc_feature    1498..1500
                     /label=CrtB start
     misc_feature    1479..1481
                     /label=CrtI stop
     misc_feature    2428..2430
                     /label=CrtB stop
ORIGIN
        1 ctcatcgtta aagagcgact acatgaaacc aactacggta attggtgcag gcttcggtgg
       61 cctggcactg gcaattcgtc tacaagctgc ggggatcccc gtcttactgc ttgaacaacg
      121 tgataaaccc ggcggtcggg cttatgtcta cgaggatcag gggtttacct ttgatgcagg
      181 cccgacggtt atcaccgatc ccagtgccat tgaagaactg tttgcactgg caggaaaaca
      241 gttaaaagag tatgtcgaac tgctgccggt tacgccgttt taccgcctgt gttgggagtc
      301 agggaaggtc tttaattacg ataacgatca aacccggctc gaagcgcaga ttcagcagtt
      361 taatccccgc gatgtcgaag gttatcgtca gtttctggac tattcacgcg cggtgtttaa
      421 agaaggctat ctaaagctcg gtactgtccc ttttttatcg ttcagagaca tgcttcgcgc
      481 cgcacctcaa ctggcgaaac tgcaagcatg gagaagcgtt tacagtaagg ttgccagtta
      541 catcgaagat gaacatctgc gccaggcgtt ttctttccac tcgctgttgg tgggcggcaa
      601 tcccttcgcc acctcatcca tttatacgtt gatacacgcg ctggagcgtg agtggggcgt
      661 ctggtttccg cgtggcggca ccggcgcatt agttcagggg atgataaagc tgtttcagga
      721 tctgggtggc gaagtcgtgt taaacgccag agtcagccat atggaaacga caggaaacaa
      781 gattgaagcc gtgcatttag aggacggtcg caggttcctg acgcaagccg tcgcgtcaaa
      841 tgcagatgtg gttcatacct atcgcgacct gttaagccag caccctgccg cggttaagca
      901 gtccaacaaa ctgcaaacta agcgcatgag taactctctg tttgtgctct attttggttt
      961 gaatcaccat catgatcagc tcgcgcatca cacggtttgt ttcggcccgc gttaccgcga
     1021 gctgattgac gaaattttta atcatgatgg cctcgcagag gacttctcac tttatctgca
     1081 cgcgccctgt gtcacggatt cgtcactggc gcctgaaggt tgcggcagtt actatgtgtt
     1141 ggcgccggtg ccgcatttag gcaccgcgaa cctcgactgg acggttgagg ggccaaaact
     1201 acgcgaccgt atttttgcgt accttgagca gcattacatg cctggcttac ggagtcagct
     1261 ggtcacgcac cggatgttta cgccgtttga ttttcgcgac cagcttaatg cctatcatgg
     1321 ctcagccttt tctgtggagc ccgttcttac ccagagcgcc tggtttcggc cgcataaccg
     1381 cgataaaacc attactaatc tctacctggt cggcgcaggc acgcatcccg gcgcaggcat
     1441 tcctggcgtc atcggctcgg caaaagcgac agcaggtttg atgctggagg atctgatatg
     1501 aataatccgt cgttactcaa tcatgcggtc gaaacgatgg cagttggctc gaaaagtttt
     1561 gcgacagcct caaagttatt tgatgcaaaa acccggcgca gcgtactgat gctctacgcc
     1621 tggtgccgcc attgtgacga tgttattgac gatcagacgc tgggctttca ggcccggcag
     1681 cctgccttac aaacgcccga acaacgtctg atgcaacttg agatgaaaac gcgccaggcc
     1741 tatgcaggat cgcagatgca cgaaccggcg tttgcggctt ttcaggaagt ggctatggct
     1801 catgatatcg ccccggctta cgcgtttgat catctggaag gcttcgccat ggatgtacgc
     1861 gaagcgcaat acagccaact ggatgatacg ctgcgctatt gctatcacgt tgcaggcgtt
     1921 gtcggcttga tgatggcgca aatcatgggc gtgcgggata acgccacgct ggaccgcgcc
     1981 tgtgaccttg ggctggcatt tcagttgacc aatattgctc gcgatattgt ggacgatgcg
     2041 catgcgggcc gctgttatct gccggcaagc tggctggagc atgaaggtct gaacaaagag
     2101 aattatgcgg cacctgaaaa ccgtcaggcg ctgagccgta tcgcccgtcg tttggtgcag
     2161 gaagcagaac cttactattt gtctgccaca gccggcctgg cagggttgcc cctgcgttcc
     2221 gcctgggcaa tcgctacggc gaagcaggtt taccggaaaa taggtgtcaa agttgaacag
     2281 gccggtcagc aagcctggga tcagcggcag tcaacgacca cgcccgaaaa attaacgctg
     2341 ctgctggccg cctctggtca ggcccttact tcccggatgc gggctcatcc tccccgccct
     2401 gcgcatctct ggcagcgccc gctctaataa
//