BBa_I742135
1
BBa_I742135
Natural RBS + Recombinase XerC of the XerCD complex.
2007-10-21T11:00:00Z
2015-08-31T04:08:03Z
XerC is an innate E. coli K12 locus.
Xer C is activated by a septum-translocated FtsK at cell division. It pairs with XerD to make an activated complex which cleaves or inverts sequences at dif-sites, depending on dif-site directionality. See Edinburgh project 2007 for details.
It turns out that XerC overexpression is a very effective way of not getting any colonies on your agar-plates. Therefore the Edinburgh 2007 team never incorperated it into its final construct.
false
false
_123_
0
1968
9
Not in stock
false
The coding XerC sequence contains none of the restriction sites used when biobricking. -13bp upstream sequence included for RBS reasons.
false
Caroline Dahl
BBa_I742135_sequence
1
ggattgaacgcgtatgaccgatttacacaccgatgtagaacgctacctacgttatctgagcgtggagcgccagcttagcccgataaccctgcttaactaccagcgtcagcttgaggcgatcatcaattttgccagcgaaaacggcctgcaaagctggcagcaatgtgatgtgacgatggtgcgcaattttgctgtacgcagtcgccgtaaagggctgggagcagcaagtctggcgttacggctttctgcgctacgtagcttttttgactggctggtcagccagaacgaactcaaagctaacccggcgaaaggtgtttcggcaccgaaagcgccgcgtcatctgccgaaaaacatcgacgtcgacgatatgaatcggctgctggatattgatatcaatgatcccctcgctgtacgcgaccgtgcaatgctggaagtgatgtacggcgcgggtctgcgtctttctgagctggtggggctggatattaaacacctcgacctggagtctggtgaagtgtgggttatggggaaaggcagcaaagagcgccgcctgccgattggtcgcaacgctgtggcgtggattgagcactggcttgatttgcgcgacctgtttggtagcgaagacgacgcgctttttctgtcgaaactgggcaagcgtatctccgcgcgtaatgtgcagaaacgctttgccgaatggggcataaaacaagggctgaataatcacgttcatccgcataaattacgtcactcgttcgccacgcatatgctggagtcgagcggcgatcttcgtggtgtgcaggagctgctgggtcatgccaacctctccaccacgcaaatctatactcatcttgattttcaacaccttgcctcggtgtacgatgcggcgcatccacgcgccaaacgggggaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z