##gff-version 3
##sequence-region BBa_I742140 1 6896
BBa_I742140	.	ribosome_entry_site	4250	4259	.	+	0	ID=BBa_J15001;Name=BBa_J15001
BBa_I742140	.	sequence_feature	4250	4252	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_I742140	.	ribosome_entry_site	4253	4259	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_I742140	.	ribosome_entry_site	6047	6056	.	+	0	ID=BBa_J15001;Name=BBa_J15001
BBa_I742140	.	sequence_feature	6047	6049	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_I742140	.	ribosome_entry_site	6050	6056	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_I742140	.	engineered_region	1	1558	.	+	0	ID=BBa_I742105;Name=BBa_I742105
BBa_I742140	.	CDS	17	1558	.	+	0	ID=annotation1949956;Name=sam5 coding region;Parent=BBa_I742105
BBa_I742140	.	ribosome_entry_site	1	16	.	+	0	ID=annotation1949955;Name=rbs;Parent=BBa_I742105
BBa_I742140	.	engineered_region	1567	3116	.	+	0	ID=BBa_I742106;Name=BBa_I742106
BBa_I742140	.	ribosome_entry_site	1567	1580	.	+	0	ID=annotation1949973;Name=native rbs;Parent=BBa_I742106
BBa_I742140	.	CDS	1581	3116	.	+	0	ID=annotation1949974;Name=Sam8 coding region;Parent=BBa_I742106
BBa_I742140	.	ribosome_entry_site	3125	3134	.	+	0	ID=BBa_J15001;Name=BBa_J15001
BBa_I742140	.	sequence_feature	3125	3127	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_I742140	.	ribosome_entry_site	3128	3134	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_I742140	.	CDS	6063	6896	.	+	0	ID=BBa_I742112;Name=BBa_I742112
BBa_I742140	.	stop_codon	6891	6896	.	+	0	ID=annotation1949784;Name=stop;Parent=BBa_I742112
BBa_I742140	.	start_codon	6063	6065	.	+	0	ID=annotation1949783;Name=start;Parent=BBa_I742112
BBa_I742140	.	CDS	6063	6890	.	+	0	ID=annotation1949782;Name=ech coding region;Parent=BBa_I742112
BBa_I742140	.	CDS	4266	6038	.	+	0	ID=BBa_I742114;Name=BBa_I742114
BBa_I742140	.	CDS	4266	6038	.	+	0	ID=annotation1949792;Name=fcs coding region;Parent=BBa_I742114
BBa_I742140	.	CDS	3141	4241	.	+	0	ID=BBa_I742107;Name=BBa_I742107
BBa_I742140	.	CDS	3141	4241	.	+	0	ID=annotation1949817;Name=COMT coding region;Parent=BBa_I742107
>BBa_I742140
acggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgag
gagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctgg
ccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggt
gcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcg
cgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcg
gcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacg
ggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgc
gtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgacca
acgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactc
gcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctg
tcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacg
acgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgc
ggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcag
gcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgt
gggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcat
caaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgac
gtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgc
tgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcat
ccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtcc
gagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataatactagaggaggttgggacaag
atgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggt
gggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacct
gaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggag
cagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctct
ggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaa
caaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcg
ctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgc
tcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcct
cgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgacc
ctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcgg
agtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtg
cgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgc
caggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccg
accagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaa
cggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcg
ttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacg
tgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggt
gggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccg
ctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcg
tcgacgaggcagacgggaaggatttcggataataatactagagctcaaggaggtactagatgggttcaacaggtgaaac
tcaaataacaccaacccacatatcagatgaagaagcaaacctcttcgccatgcaactagcaagtgcttcagttcttccc
atgattttgaaatcagctcttgaacttgatctcttagaaatcattgctaaagctggacctggtgctcaaatttcaccta
ttgaaattgcttctcagctaccaacaactaaccctgatgcaccagttatgttggaccgaatgttgcgtctcttggcttg
ttacataatcctcacatgttcagttcgtactcaacaagatggaaaggttcagagactttatggtttggctactgttgct
aagtatttggttaagaatgaagatggtgtatccatttctgctcttaatctcatgaatcaggataaagtgctcatggaaa
gctggtaccacctaaaagatgcagtccttgatgggggcattccattcaacaaggcttatggaatgacagcctttgaata
ccatggaacagatccaaggtttaacaaggttttcaacaaggggatgtctgatcactctaccatcacaatgaagaaaatt
cttgagacctacacaggttttgaaggccttaaatctcttgttgatgtaggtggtggtactggagctgtaattaacacga
ttgtctcaaaatatcccactataaagggtataaattttgatttaccccatgtcattgaagatgctccatcttatccagg
agttgagcatgttggtggagacatgtttgtcagtattccaaaggctgatgctgtttttatgaagtggatttgtcatgac
tggagtgatgagcactgcttgaaatttttgaagaactgctatgaggcactgccagacaatggaaaagtgattgtggcag
aatgcatacttccagtggctccagattcaagcctggccacaaaaggtgtggttcacattgatgtgatcatgttggctca
taatcctggtgggaaagagagaacacaaaaagagtttgaggatcttgccaaaggtgctggattccaaggtttcaaagtc
cattgtaatgctttcaacacatacatcatggagtttcttaagaaggtttaataatactagagctcaaggaggtactaga
tgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccgga
acagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgt
gcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaac
atctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctca
ggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcag
cgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagatta
gctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgc
gaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccag
cagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacct
ttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgccca
gggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaa
ctggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcgg
caggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggc
gggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctg
ccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgc
cgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaa
actggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggc
gtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcgg
caccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcgga
agcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaa
gcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccg
ataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatcc
gaccattctgcgtgcgggtccgaaaccgtaataatactagagctcaaggaggtactagatgagcaaatatgaaggccgt
tggaccaccgtgaaagtggaaattgaagaaggcattgcctgggttattctgaaccgtccggaaaaacgtaacgccatga
gcccgaccctgaaccgtgaaatgattgatgtgctggaaaccctggaacaggatccggcggcgggtgttctggttctgac
cggtgcgggtgaagcgtggaccgcgggcatggatctgaaagaatatttccgtgaagtggatgcgggtccggaaattctg
caagaaaaaattcgtcgcgaagcgagccagtggcagtggaaactgctgcgtatgtatgcgaaaccgaccattgccatgg
tgaacggctggtgctttggcggcggttttagcccgctggttgcgtgcgatctggccatttgcgcggatgaagcgacctt
tggcctgagcgagattaactggggcattccgccgggtaacctggtgagcaaagccatggcggataccgtgggccatcgt
cagagcctgtattatatcatgaccggcaaaacctttggtggccagaaagcggcggaaatgggcctggtgaacgaaagcg
tgccgctggcccagctgcgtgaagtgaccattgaactggcccgtaacctgctggaaaaaaatccggtggtgctgcgtgc
ggcgaaacatggctttaaacgttgccgtgaactgacctgggaacagaacgaagattatctgtacgcgaaactggatcag
agccgtctgctggataccgaaggcggccgtgaacagggcatgaaacagtttctggatgataaaagcattaaaccgggcc
tgcaagcgtataaacgttaataa